Flow cytometry
Our flow cytometry platform is focused on the application of cytometry methods to the characterization of immune cells. Our attempts are to stain the cells on the cell surface and intracellularly to depict the ones which are of interest for us. We have been able to establish antibody panels for human immune cells characterization using more than 20 fluorescence dyes simultaneously by means of our 3 laser CYTEK Aurora full spectrum flow cytometer. The advantage of this multicolour staining is that only very low sample volumes are needed. We are capable of sorting the cells of interest on our BioRad S3e Cell Sorter using up to 4 fluorescence channels. We are constantly improving our staining assays to depict the activation status of immune cells (including cytokine expression, transcription factors, mRNA expression and phospho-flow). We have established new methods to evaluate the influence of environmental chemicals and microorganisms like microbiota and viruses on the activation of certain types of immune cells including T helper cells such as regulatory T cells and cytotoxic T cells e.g. mucosal-associated invariant T cells. For data analysis and presentation, we use the professional software FlowJo and FlowLogic.
Since September 2021, the department also has a new CyTOF (SAB aid, Project no. 100588139) and Hyperion device from FLUIDIGM (Cytometry Time of Flight CyTOF/Hyperion Imaging System from Fluidigm). With the help of this novel and innovative imaging mass cytometry, we would like to examine immune cell profiles from cohort samples (e.g. mother-child cohorts), tissue samples (e.g. placenta) and organoids after exposure to environmental chemicals in a thorough and comprehensive manner. With this unique system, the smallest amounts of blood and tissue-specific cells can be characterized on the basis of many parameters (including signal transduction cascades). Also functional properties can be derived.
Photo: Beate Fink/UFZ
This measure is co-financed with tax revenues on the basis of the budget decided by the Saxon state parliament.
We have more than 15 years of experience using flow cytometry for different applications. Our platform is open for collaboration - we can find the staining solution to most immune cells. We like new challenges and we constantly develop our skills portfolio.
Department News
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The 25th Interdisziplinäre Kinderimmunologische Arbeitstreffen (KIAT) took place on November 11th in Leipzig. Prof. Ana Zenclussen and Dr. Gunda Herberth gave talks about immunology during pregnancy, the role of T cells and current results from cohort studies.
f.l.t.r.: Prof. Dr. Ulrich Sack, Dr. Gunda Herberth, Prof. Ana C. Zenclussen, Prof. Dr. Michael Borte
We welcome our new project coordinator Susann Hähnel and the new medical doctoral students Elisabeth, Jonas and Lukas in our team!
Congratulations to our colleague Dr. Violeta Stojanovska for being the 3rd winner of the Participants Choice Awards at the Helmholtz Imaging Conference 2022!
The first joint workshop with our Argentinian cooperation partners took place on 11 and 12 July. The participants were able to discuss the progress of the DFG project "Influence of endocrine disruptors on reproductive processes mediated by immune cells of the innate immune system ", methods and to-dos. There was also space to plan further joint collaborations. We thank all participants for the exciting and productive exchange!
Back: Tobias Kretschmer, Anne Schumacher, Horacio Rodriguez, Ana Zenclussen, Nicole Meyer, Maria Zenclussen, Florence Fischer
Congratulations to our colleague Dr. Violeta Stojanovska for being the 1st prize European Organoid Research Grant winner!
@biotechne
To what extent do older siblings influence the healthy development of children? Answers from our LiNA study can be found in our latest press release .
Photo: PD Dr. Stefan Fest
Prof. Ana Zenclussen and Dr. Violeta Stojanovska , together with PD Dr. Stefan Fest , took part in the annual conference of the American Society for Reproductive Immunology in Nashville . In addition to presenting their own results on the topic of "Characterization of 3D trophoblast spheroids as a model of placenta functionality", our scientists also had the opportunity to finally exchange ideas in person with colleagues from all over the world.
We congratulate Prof. Ana Zenclussen on her appointment as a member of the BfR Commission on Contaminants in the Food Chain for the session period 2022 to 2025 and wish her every success!
Latest publications
Index: 2023 (2) 2022 (39)
You could use our publication index for further requests.
Hofsink, N., Dijkstra, D.J., Stojanovska, V. , Scherjon, S.A., Plösch, T. (2023):Preeclampsia-induced alterations in brain and liver gene expression and DNA methylation patterns in fetal mice J. Dev. Orig. Health Dis. 14 (1), 146 - 151full text (doi) Wang, M., Flexeder, C., Kilanowski, A., Kress, S., Herberth, G. , Schikowski, T., Peters, A., Standl, M. (2023):Changes in sleep duration and sleep difficulties from adolescence to young adulthood and the risk of obesity: Bidirectional evidence in the GINIplus and LISA studies Sleep Med. 101 , 401 - 410full text (doi) to index Amici, F., Röder, S. , Kiess, W., Borte, M., Zenclussen, A.C. , Widdig, A., Herberth, G. (2022):Maternal stress, child behavior and the promotive role of older siblings BMC Public Health 22 , art. 863full text (doi) Bauer, M. , Fink, B. , Anderegg, U., Röder, S. , Zenclussen, A.C. (2022):IL17F expression as an early sign of oxidative stress-induced cytotoxicity/apoptosisGenes 13 (10), art. 1739full text (doi) Buchenauer, L. , Junge, K.M. , Haange, S.-B., Simon, J.C., von Bergen, M., Hoh, A.-L., Aust, G., Zenclussen, A.C. , Stangl, G.I., Polte, T. (2022):Glyphosate differentially affects the allergic immune response across generations in mice Sci. Total Environ. 850 , art. 157973full text (doi) Busse, M., Langwisch, S., Tedford, K., Fischer, K.-D., Zenclussen, A.C. (2022):Maternal B cell signaling orchestrates fetal development in mice Development 149 (8), dev.199783full text (doi) Busse, M., Scharm, M., Oettel, A., Redlich, A., Costa, S.-D., Zenclussen, A.C. (2022):Enhanced S100B expression in T and B lymphocytes in spontaneous preterm birth and preeclampsia J. Perinat. Med. 50 (2), 157 - 166full text (doi) Busse, M., Zenclussen, A.C. (2022):IL-10 producing B cells protect against LPS-induced murine preterm birth by promoting PD1- and ICOS-expressing T cells Cells 11 (17), art. 2690full text (doi) Campe, K.-N.J., Redlich, A., Zenclussen, A.C. , Busse, M. (2022):An increased proportion of progesterone receptor A in peripheral B cells from women who ultimately underwent spontaneous preterm birth J. Reprod. Immunol. 154 , art. 103756full text (doi) Fischer, F. , Schumacher, A. , Meyer, N. , Fink, B. , Bauer, M. , Stojanovska, V. , Zenclussen, A.C. (2022):An old friend with a new face: YB-1 and its role in healthy pregnancy and pregnancy-associated complications Front. Cell. Dev. Biol. 10 , art. 1039206full text (doi) Fraser, R., Zenclussen, A.C. (2022):Killer timing: The temporal uterine natural killer cell differentiation pathway and implications for female reproductive health Front. Endocrinol. 13 , art. 904744full text (doi) Ganther, M., Lippold, E., Bienert, M.D., Bouffaud, M.-L., Bauer, M. , Baumann, L., Bienert, G.P., Vetterlein, D., Heintz-Buschart, A., Tarkka, M.T. (2022):Plant age and soil texture rather than the presence of root hairs cause differences in maize resource allocation and root gene expression in the field Plants 11 (21), art. 2883full text (doi) Greimel, E., Adams, L., Zsigo, C., Berdel, D., von Berg, A., Koletzko, S., Bauer, C.-P., Schikowski, T., Herberth, G. , Heinrich, J., Schulte-Körne, G., Standl, M. (2022):Psychopathological symptoms as precursors of depressive symptoms in adolescence: a prospective analysis of the GINIplus and LISA birth cohort studies Soc. Psychiatry Psychiatr. Epidemiol. 57 (8), 1627 - 1639full text (doi) Junge, K.M. , Buchenauer, L. , Strunz, S. , Seiwert, B., Thürmann, L., Rolle-Kampczyk, U.E., Röder, S. , Borte, M., Kiess, W., von Bergen, M., Simon, J.C., Zenclussen, A.C. , Schöneberg, T., Stangl, G.I., Herberth, G. , Lehmann, I., Reemtsma, T., Polte, T. (2022):Effects of exposure to single and multiple parabens on asthma development in an experimental mouse model and a prospective cohort study Sci. Total Environ. 814 , art. 152676full text (doi) Juskiene, I., Prokopciuk, N., Franck, U. , Valiulis, A., Valskys, V., Mesceriakova, V., Kvedariene, V., Valiulyte, I., Poluzioroviene, E., Sauliene, I., Valiulis, A. (2022):Indoor air pollution effects on pediatric asthma are submicron aerosol particle–dependent Eur. J. Pediatr. 181 (6), 2469 - 2480full text (doi) Kempf, E., Landgraf, K., Stein, R., Hanschkow, M., Hilbert, A., Abou Jamra, R., Boczki, P., Herberth, G. , Kühnapfel, A., Tseng, Y.-H., Stäubert, C., Schöneberg, T., Kühnen, P., Rayner, N.W., Zeggini, E., Kiess, W., Blüher, M., Körner, A. (2022):Aberrant expression of agouti signaling protein (ASIP ) as a cause of monogenic severe childhood obesity Nat. Metab. 4 , 1697 - 1712full text (doi) Kilanowski, A., Thiering, E., Wang, G., Kumar, A., Kress, S., Flexeder, C., Bauer, C.-P., Berdel, D., von Berg, A., Bergström, A., Gappa, M., Heinrich, J., Herberth, G. , Koletzko, S., Kull, I., Melen, E., Schikowski, T., Peters, A., Standl, M. (2022):Allergic disease trajectories up to adolescence: Characteristics, early-life and genetic determinants Allergy full text (doi) Kocatürk, E., Podder, I., Zenclussen, A.C. , Kasperska Zajac, A., Elieh-Ali-Komi, D., Church, M.K., Maurer, M. (2022):Urticaria in pregnancy and lactation Front. Allergy 3 , art. 892673full text (doi) Krause, J.L. , Engelmann, B., Nunes da Rocha, U., Pierzchalski, A. , Chang, H.D., Zenclussen, A.C. , von Bergen, M., Rolle-Kampczyk, U., Herberth, G. (2022):MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols Environ. Int. 158 , art. 106985full text (doi) Kretschmer, T. , Turnwald, E.-M., Janoschek, R., Wohlfarth, M., Handwerk, M., Dötsch, J., Hucklenbruch-Rother, E., Appel, S. (2022):Treatment of high fat diet‐induced obese pregnant mice with IL‐6 receptor antibody does not ameliorate placental function and fetal growth restriction Am. J. Reprod. Immunol. 88 (1), e13564full text (doi) Lentz, L.S., Stutz, A.J., Meyer, N. , Schubert, K., Karkossa, I., von Bergen, M., Zenclussen, A.C. , Schumacher, A. (2022):Human chorionic gonadotropin promotes murine Treg cells and restricts pregnancy-harmful proinflammatory Th17 responses Front. Immunol. 13 , art. 989247full text (doi) Lokossou, G.A.G., Kouakanou, L., Schumacher, A. , Zenclussen, A.C. (2022):Human breast milk: From food to active immune response with disease protection in infants and mothers Front. Immunol. 13 , art. 849012full text (doi) Marb, A., Libuda, L., Standl, M., Koletzko, S., Bauer, C.-P., Schikowski, T., Berdel, D., von Berg, A., Herberth, G. , Bühlmeier, J., Harris, C.P. (2022):Obesogenic eating behaviour and dietary intake in German children and adolescents: results from the GINIplus and LISA birth cohort studies Eur. J. Clin. Nutr. 76 , 1478 - 1485full text (doi) Markevych, I., Astell-Burt, T., Altug, H., Triebner, K., Standl, M., Flexeder, C., Heinrich, J., Schikowski, T., Koletzko, S., Herberth, G. , Bauer, C.-P., von Berg, A., Berdel, D., Feng, X. (2022):Residential green space and age at menarche in German and Australian adolescent girls: A longitudinal study Int. J. Hyg. Environ. Health. 240 , 113917full text (doi) Meijer, M., Klein, M., Caramaschi, D., Mulder, R., Cosin, M., Lu, X., Zhang, Y., Röder, S. , Zilich, L., Huels, A., Hartman, C., Snieder, H., Bustamante, M., Herberth, G. , Franke, B., Copeland, B., Aberg, K., van den Oord, E. (2022):Meta-analysis of cell type-sSpecific DNA methylation of childhood attention-deficit/hyperactivity disorder symptoms Biol. Psychiatry 91 (9 (Suppl.)), S111 - S112full text (doi) Musiol, S., Harris, C.P., Karlina, R., Gostner, J.M., Rathkolb, B., Schnautz, B., Schneider, E., Mair, L., Elorduy Vergara, E., Flexeder, C., Koletzko, S., Bauer, C.-P., Schikowski, T., Berdel, D., von Berg, A., Herberth, G. , Rozman, J., Hrabe de Angelis, M., Standl, M., Schmidt-Weber, C.B.., Ussar, S., Alessandrini, F. (2022):Dietary digestible carbohydrates are associated with higher prevalence of asthma in humans and with aggravated lung allergic inflammation in mice Allergy full text (doi) Röder, S. , Herberth, G. , Zenclussen, A.C. , Bauer, M. (2022):EpiVisR: exploratory data analysis and visualization in epigenome-wide association analyses BMC Bioinformatics 23 , art. 292full text (doi) Romero Perez, R.V., Zarzycka, A., Preussner, M., Fischer, F. , Roth, K., Keber, C.U., Suryamohan, K., Raifer, H., Luu, M., Leister, H., Bertrams, W., Klein, M., Shams-Eldin, H., Jacob, R., Mollenkopf, H.-J., Rajalingam, K., Visekruna, A., Steinhoff, U. (2022):A defined bacterial community restores immunity in germ-free mice via maturation of the intestinal vascular system Eur. J. Immunol. 52 (S1), 49 - 50full text (doi) Romero, R., Zarzycka, A., Preussner, M., Fischer, F. , Hain, T., Herrmann, J.-P., Roth, K., Keber, C.U., Suryamohan, K., Raifer, H., Luu, M., Leister, H., Bertrams, W., Klein, M., Shams-Eldin, H., Jacob, R., Mollenkopf, H.-J., Rajalingam, K., Visekruna, A., Steinhoff, U. (2022):Selected commensals educate the intestinal vascular and immune system for immunocompetence Microbiome 10 , art. 158full text (doi) Sammallahti, S., Koopman‑Verhoeff, M.E., Binter, A.-C., Mulder, R.H., Cabré‑Riera, A., Kvist, T., Malmberg, A.L.K., Pesce, G., Plancoulaine, S., Heiss, J.A., Rifas‐Shiman, S.L., Röder, S.W. , Starling, A.P., Wilson, R., Guerlich, K., Haftorn, K.L., Page, C.M., Luik, A.I., Tiemeier, H., Felix, J.F., Raikkonen, K., Lahti, J., Relton, C.L., Sharp, G.C., Waldenberger, M., Grote, V., Heude, B., Annesi‑Maesano, I., Hivert, M.-F., Zenclussen, A.C. , Herberth, G. , Dabelea, D., Grazuleviciene, R., Vafeiadi, M., Håberg, S.E., London, S.J., Guxens, M., Richmond, R.C., Cecil, C.A.M. (2022):Longitudinal associations of DNA methylation and sleep in children: a meta‑analysis Clin. Epigenetics 14 , art. 83full text (doi) Schicketanz, J., Röder, S. , Herberth, G. , Kabisch, S., Lakes, T. (2022):On foot or by car: what determines children’s active school travel? Child. Geogr. 20 (2), 174 - 188full text (doi) Schmitz, K., Turnwald, E.-M., Kretschmer, T. , Janoschek, R., Bae-Gartz, I., Voßbrecher, K., Kammerer, M.D., Köninger, A., Gellhaus, A., Handwerk, M., Wohlfarth, M., Gründemann, D., Hucklenbruch-Rother, E., Dötsch, J., Appel, S. (2022):Metformin prevents key mechanisms of obesity-related complications in visceral white adipose tissue of obese pregnant mice Nutrients 14 (11), art. 2288full text (doi) Scholz, S., Brack, W., Escher, B.I., Hackermüller, J., Liess, M., von Bergen, M., Wick, L.Y., Zenclussen, A.C. , Altenburger, R. (2022):The EU chemicals strategy for sustainability: an opportunity to develop new approaches for hazard and risk assessment Arch. Toxicol. 96 (8), 2381 - 2386full text (doi) Schütte, O. , Bachmann, L. , Shivappa, N., Hebert, J.R., Felix, J.F., Röder, S.W. , Sack, U., Borte, M., Kiess, W., Zenclussen, A.C. , Stangl, G.I., Herberth, G. , Junge, K.M. (2022):Pro-inflammatory diet pictured in children with atopic dermatitis or food allergy: nutritional data of the LiNA cohort Front. Nutr. 9 , art. 868872full text (doi) Stojanovska, V. , Arnold, S. , Bauer, M. , Voss, H., Fest, S. , Zenclussen, A.C. (2022):Characterization of three-dimensional trophoblast spheroids: an alternative model to study the physiological properties of the placental unit Cells 11 (8), art. 2884full text (doi) Stojanovska, V. , Voss, H., Fest, S. , Zenclussen, A.C. (2022):P45: Characterization of 3D trophoblast spheroids as a model of placenta functionality Am. J. Reprod. Immunol. 87 (S1), 98 - 99full text (doi) Stutz, A., Nishanth, G., Zenclussen, A.C. , Schumacher, A. (2022):Partial otubain 1 deficiency compromises fetal well-being in allogeneic pregnancies despite no major changes in the dendritic cell and T cell compartment BMC Res. Notes 15 , art. 341full text (doi) van Meel, E.R., Mensink-Bout, S.M., den Dekker, H.T., Ahluwalia, T.S., Annesi-Maesano, I., Arshad, S.H., Baïz, N., Barros, H., von Berg, A., Bisgaard, H., Bønnelykke, K., Carlsson, C.J., Casas, M., Chatzi, L., Chevrier, C., Dalmeijer, G., Dezateux, C., Duchen, K., Eggesbø, M., van der Ent, C., Fantini, M., Flexeder, C., Frey, U., Forastiere, F., Gehring, U., Gori, D., Granell, R., Griffiths, L.J., Inskip, H., Jerzynska, J., Karvonen, A.M., Keil, T., Kelleher, C., Kogevinas, M., Koppen, G., Kuehni, C.E., Lambrechts, N., Lau, S., Lehmann, I. , Ludvigsson, J., Magnus, M.C., Mélen, E., Mehegan, J., Mommers, M., Nybo Andersen, A.-M., Nystad, W., Pedersen, E.S.L., Pekkanen, J., Peltola, V., Pike, K.C., Pinot de Moira, A., Pizzi, C., Polanska, K., Popovic, M., Porta, D., Roberts, G., Santos, A.C., Schultz, E.S., Standl, M., Sunyer, J., Thijs, C., Toivonen, L., Uphoff, E., Usemann, J., Vafeidi, M., Wright, J., de Jongste, J.C., Jaddoe, V.W.V., Duijts, L. (2022):Early-life respiratory tract infections and the risk of school-age lower lung function and asthma: a meta-analysis of 150 000 European children Eur. Resp. J. 60 (4), art. 2102395full text (doi) Wang, J., Tischer, C., Standl, M., Weidinger, S., von Berg, A., Herberth, G. , Yew, Y.W., Heinrich, J., Schmitt, J., Apfelbacher, C. (2022):Lifetime prevalence and determinants of hand eczema in an adolescent population in Germany: 15-year follow-up of the LISA cohort study J. Eur. Acad. Dermatol. Venereol. 36 (4), 547 - 556full text (doi) Weißenborn, C., von Lenthe, S., Hinz, N., Langwisch, S., Busse, M., Schumacher, A. , Zenclussen, A.C. , Fest, S. (2022):Depletion of Foxp3+ regulatory T cells but not the absence of CD19+IL-10+ regulatory B cells hinders tumor growth in a para-orthotopic neuroblastoma mouse model Int. J. Cancer 151 (11), 2021 - 2042full text (doi) Zhang, N. , Schumacher, A. , Fink, B. , Bauer, M. , Zenclussen, A.C. , Meyer, N. (2022):Insights into early-pregnancy mechanisms: Mast cells and chymase CMA1 shape the phenotype and modulate the functionality of human trophoblast cells, vascular smooth-muscle cells and endothelial cells Cells 11 (7), art. 1158full text (doi) to index
Projects
We participate in the joint research project SaxoChiLD.
@SaxoChiLD
We participate in the joint research project
@CoViPa
