Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.3390/cells13050376 |
Licence  |
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| Title (Primary) | Absence of heme oxygenase-1 affects trophoblastic spheroid implantation and provokes dysregulation of stress and angiogenesis gene expression in the uterus |
| Author | Zenclussen, M.L.; Ulrich, S.; Bauer, M.; Fink, B.; Zenclussen, A.C.; Schumacher, A.; Meyer, N. |
| Source Titel | Cells |
| Year | 2024 |
| Department | IMMU |
| Volume | 13 |
| Issue | 5 |
| Page From | art. 376 |
| Language | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://www.mdpi.com/article/10.3390/cells13050376/s1 |
| Keywords | heme oxygenase-1; implantation; pregnancy; angiogenesis |
| Abstract | The enzyme heme oxygenase-1 (HO-1) is pivotal in reproductive processes, particularly in placental and vascular development. This study investigated the role of HO-1 and its byproduct, carbon monoxide (CO), in trophoblastic spheroid implantation. In order to deepen our understanding of the role of HO-1 during implantation, we conducted in vivo experiments on virgin and pregnant mice, aiming to unravel the cellular and molecular mechanisms. Using siRNA, HO-1 was knocked down in JEG-3 and BeWo cells and trophoblastic spheroids were generated with or without CO treatment. Adhesion assays were performed after transferring the spheroids to RL-95 endometrial epithelial cell layers. Additionally, angiogenesis, stress, and toxicity RT2-Profiler™ PCR SuperArray and PCR analyses were performed in uterine murine samples. HO-1 knockdown by siRNA impeded implantation in the 3D culture model, but this effect could be reversed by CO. Uteruses from virgin Hmox1−/− females exhibited altered expression of angiogenesis and stress markers. Furthermore, there was a distinct expression pattern of cytokines and chemokines in uteruses from gestation day 14 in Hmox1−/− females compared to Hmox1+/+ females. This study strongly supports the essential role of HO-1 during implantation. Moreover, CO appears to have the potential to compensate for the lack of HO-1 during the spheroid attachment process. The absence of HO-1 results in dysregulation of angiogenesis and stress-related genes in the uterus, possibly contributing to implantation failure |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28806 |
| Zenclussen, M.L., Ulrich, S., Bauer, M., Fink, B., Zenclussen, A.C., Schumacher, A., Meyer, N. (2024): Absence of heme oxygenase-1 affects trophoblastic spheroid implantation and provokes dysregulation of stress and angiogenesis gene expression in the uterus Cells 13 (5), art. 376 10.3390/cells13050376 |
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