Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.toxrep.2023.02.017
Licence creative commons licence
Title (Primary) Bisphenols, but not phthalate esters, modulate gene expression in activated human MAIT cells in vitro
Author Krause, J.L.; Pierzchalski, A.; Chang, H.D.; Zenclussen, A.C.; Bauer, M.; Herberth, G. ORCID logo
Source Titel Toxicology Reports
Year 2023
Department IMMU
Volume 10
Page From 348
Page To 356
Language englisch
Topic T9 Healthy Planet
Supplements https://ars.els-cdn.com/content/image/1-s2.0-S221475002300029X-mmc1.docx
Keywords Bisphenols; Plasticizers; In vitro model; Phthalate ester; MAIT cells; Immunomodulation
Abstract One route of human exposure to environmental chemicals is oral uptake. This is primarily true for chemicals that may leach from food packaging materials, such as bisphenols and phthalate esters. Upon ingestion, these compounds are transported along the intestinal tract, from where they can be taken up into the blood stream or distributed to mucosal sites. At mucosal sites, mucosal immune cells and in the blood stream peripheral immune cells may be exposed to these chemicals potentially modulating immune cell functions. In the present study, we investigated the impact of three common bisphenols and two phthalate esters on mucosal-associated invariant T (MAIT) cells in vitro, a frequent immune cell type in the intestinal mucosae and peripheral blood of humans. All compounds were non-cytotoxic at the chosen concentrations. MAIT cell activation was only slightly affected as seen by flow cytometric analysis. Phthalate esters did not affect MAIT cell gene expression, while bisphenol-exposure induced significant changes. Transcriptional changes occurred in ∼ 25 % of genes for BPA, ∼ 22 % for BPF and ∼ 8 % for BPS. All bisphenols down-modulated expression of CCND2, CCL20, GZMB and IRF4, indicating an effect on MAIT cell effector function. Further, BPA and BPF showed a high overlap in modulated genes involved in cellular stress response, activation signaling and effector function suggesting that BPF may not be safe substitute for BPA.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24331
Krause, J.L., Pierzchalski, A., Chang, H.D., Zenclussen, A.C., Bauer, M., Herberth, G. (2023):
Bisphenols, but not phthalate esters, modulate gene expression in activated human MAIT cells in vitro
Toxicol. Rep. 10 , 348 - 356