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Research Focus:

Life in its essence is made up by the interplay of living matter with their environment, connected via maintenance, growth, replication, and active thermodynamic differentiation. If life wants to be understood and used for technical purposes, it is mandatory to understand the cell as its minimal unit beyond the bulk of heterogeneous populations. The research of this platform addresses questions regarding the functional boundaries of this unit with microfluidic technologies. Exploiting the physical laws at the microscale, we aim at revealing biological mechanisms that conduct the physiology of the microbial cell and its interplay with the cellular environment. The obtained knowledge enables a rational redesign of life, finally towards applications in key technologies for new materials and energy carriers.



Team

Head:

Dr. Christian Dusny

Scientific Staff:

Martin Schirmer


Scientific Staff:

Paul Böhme

Nader Gadelrab

Index:

2019 (1)
2018 (1)
2017 (3)
2016 (2)
2015 (4)

You could use our publication index for further requests.

2019 (1)

  • Dusny, C., Lohse, M., Reemtsma, T., Schmid, A., Lechtenfeld, O.J., (2019):
    Quantifying a biocatalytic product from a few living microbial cells using microfluidic cultivation coupled to FT-ICR-MS
    Anal. Chem. 91 (11), 7012 - 7018
    full text (url)
to index

2018 (1)

  • Oehling, V., Klaassen, P., Frick, O., Dusny, C., Schmid, A., (2018):
    L-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
    Biotechnol. Biofuels 11 , art. 231
    full text (url)
to index

2017 (3)

  • Delvigne, F., Baert, J., Sassi, H., Fickers, P., Grünberger, A., Dusny, C., (2017):
    Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses
    Biotechnology Journal 12 (7), art. 1600549
    full text (url)
  • Fritzsch, F.S.O., Blank, L.M., Dusny, C., Schmid, A., (2017):
    Miniaturized octupole cytometry for cell type independent trapping and analysis
    Microfluid. Nanofluid. 21 (8), art. 130
    full text (url)
  • Rosenthal, K., Oehling, V., Dusny, C., Schmid, A., (2017):
    Beyond the bulk: disclosing the life of single microbial cells
    FEMS Microbiol. Rev. 41 (6), 751 - 780
    full text (url)
to index

2016 (2)

  • Dusny, C., Schmid, A., (2016):
    The MOX promoter in Hansenula polymorpha is ultrasensitive to glucose-mediated carbon catabolite repression
    FEMS Yeast Res. 16 (6), fow067
    full text (url)
  • Dusny, C., Schmid, A., (2016):
    Neue Einblicke in die Welt isolierter Mikroben
    Biospektrum 22 (7), 708 - 711
    full text (url)
to index

2015 (4)

  • Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., Schmid, A., (2015):
    Technical bias of microcultivation environments on single-cell physiology
    Lab Chip 15 (8), 1822 - 1834
    full text (url)
  • Dusny, C., Schmid, A., (2015):
    Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes
    Environ. Microbiol. 17 (6), 1839 - 1856
    full text (url)
  • Dusny, C., Schmid, A., (2015):
    Challenging biological limits with microfluidic single cell analysis
    Microb. Biotechnol. 8 (1), 23 - 25
    full text (url)
  • Rosenthal, K., Falke, F., Frick, O., Dusny, C., Schmid, A., (2015):
    An inert continuous microreactor for the isolation and analysis of a single microbial cell
    Micromachines 6 (12), 1836 - 1855
    full text (url)
to index

There are always projects available within our research themes (see below). Projects are interesting for students with a background in biotechnology, biochemical engineering, biochemistry or related. 


Microscale analysis of bioconversion in presence of sorbents

Despite the growing interest in the application of sorption-enhanced bioprocesses, such as addition of activated carbon to bioreactors for improved bioproduction or pollutant removal, the mechanisms of sorbent-mediated chemical catchment and subsequent transfer of the reactant to the bacteria are still in the void. This applies, in particular, for technical scenarios where substrate concentrations are below threshold values for growth, maintenance, gene expression, as well as biocatalyst affinity and thus impair the performance of the process. By using microfluidic bioreactors that facilitate stringent environmental control, bacteria can be brought into close proximity or direct physical contact to sorbents loaded with substrate in order to judge on the mode of mass transfer. Based on time-lapse microscopy, growth and gene expression of individual bacterial cells brought in contact with adsorbents with different surface chemistry can be tested.