Research Focus:
Life in its essence is made up by the interplay of living matter with their environment, connected via maintenance, growth, replication, and active thermodynamic differentiation. If life wants to be understood and used for technical purposes, it is mandatory to understand the cell as its minimal unit beyond the bulk of heterogeneous populations. The research of this platform addresses questions regarding the functional boundaries of this unit with microfluidic technologies. Exploiting the physical laws at the microscale, we aim at revealing biological mechanisms that conduct the physiology of the microbial cell and its interplay with the cellular environment. The obtained knowledge enables a rational redesign of life, finally towards applications in key technologies for new materials and energy carriers.
Group Leader:
PhD Students:
Technicians:
Students:
Tejas Ravindra Mate
Lyn Möhrlin
Index:
You could use our publication index for further requests.
2023 (4)
- Garmasukis, R., Hackl, C., Dusny, C., Elsner, C., Charvat, A., Schmid, A., Abel, B. (2023):
Cryo-printed microfluidics enable rapid prototyping for optical-cell analysis
Microfluid. Nanofluid. 27 , art. 5
full text (doi) - Schirmer, M., Dusny, C. (2023):
Microbial single-cell mass spectrometry: status, challenges, and prospects
Curr. Opin. Biotechnol. 83 , art. 102977
full text (doi) - Smaluch, K., Dusny, C., Kohlheyer, D., Grünberger, A. (2023):
Mikroskalige Massenbilanzierung in mikrofluidischen Umgebungen
Biospektrum 29 (5), 534 - 535
full text (doi) - Smaluch, K., Wollenhaupt, B., Steinhoff, H., Kohlheyer, D., Grünberger, A., Dusny, C. (2023):
Assessing the growth kinetics and stoichiometry of Escherichia coli at the single-cell level
Eng. Life Sci. 23 (1), e2100157
full text (doi)
2022 (6)
- Blaha, M.E., Hasan, S., Dusny, C., Belder, D. (2022):
Fluorescence lifetime activated droplet sorting (FLADS) for label-free sorting of Synechocystis sp. PCC6803
Lab Chip 22 (8), 1604 - 1614
full text (doi) - Dusny, C. (2022):
Microfluidic single-cell analytics
In: Bahnemann, J., Grünberger, A. (eds.)
Microfluidics in biotechnology
Adv. Biochem. Eng. Biotechnol. 179
Springer Nature, p. 159 - 189
full text (doi) - Martinez, J.A., Delvenne, M., Henrion, L., Moreno, F., Telek, S., Dusny, C., Delvigne, F. (2022):
Controlling microbial co-culture based on substrate pulsing can lead to stability through differential fitness advantages
PLoS Comput. Biol. 18 (10), e1010674
full text (doi) - Wink, K., van der Loh, M., Hartner, N., Polack, M., Dusny, C., Schmid, A., Belder, D. (2022):
Quantification of biocatalytic transformations by single microbial cells enabled by tailored integration of droplet microfluidics and mass spectrometry
Angew. Chem.-Int. Edit. 61 (29), e202204098
full text (doi) - Xiong, B.-J., Kleinsteuber, S., Sträuber, H., Dusny, C., Harms, H., Wick, L.Y. (2022):
Impact of fungal hyphae on growth and dispersal of obligate anaerobic bacteria in aerated habitats
mBio 13 (3), e00769-22
full text (doi) - Xiong, B.-J., Stanley, C.E., Dusny, C., Schlosser, D., Harms, H., Wick, L.Y. (2022):
pH distribution along growing fungal hyphae at microscale
J. Fungi 8 (6), art. 599
full text (doi)
2021 (5)
- Bühler, K., Bühler, B., Klähn, S., Krömer, J.O., Dusny, C., Schmid, A. (2021):
Biocatalytic production of white hydrogen from water using cyanobacteria
In: Rögner, M. (ed.)
Photosynthesis: Biotechnological applications with microalgae
De Gruyter, Berlin ; Boston, p. 279 - 306
full text (doi) - Bühler, K., Krömer, J.O., Klähn, S., Bühler, B., Dusny, C., Schmid, A. (2021):
Weißer Wasserstoff made in Leipzig
Biospektrum 27 (3), 335
full text (doi) - Dusny, C., Schmid, A. (2021):
The metabolic flux probe (MFP)—Secreted protein as a non-disruptive information carrier for 13C-based metabolic flux analysis
Int. J. Mol. Sci. 22 (17), art. 9438
full text (doi) - Krafft, B., Tycova, A., Urban, R.D., Dusny, C., Belder, D. (2021):
Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water
Electrophoresis 42 (1-2), 86 - 94
full text (doi) - Xiong, B.-J., Dusny, C., Wang, L., Appel, J., Lindstaedt, K., Schlosser, D., Harms, H., Wick, L. (2021):
Illuminate the hidden: in vivo mapping of microscale pH in the mycosphere using a novel whole-cell biosensor
ISME Commun. 1 , art. 75
full text (doi)
2020 (3)
- Dusny, C., Grünberger, A. (2020):
Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding
Curr. Opin. Biotechnol. 63 , 26 - 33
full text (doi) - Lohse, M., Dusny, C., Kaesler, J., Lechtenfeld, O.J. (2020):
MRMS powered single cell metabolomics – Quantification of picogram amounts of a biocatalytic product from few living cells
Bruker Application Note 05-2020, MRMS-70, 1877441
Bruker Daltonics, 6 pp.
full text (doi) - Schirmer, M., Wink, K., Ohla, S., Belder, D., Schmid, A., Dusny, C. (2020):
Conversion efficiencies of a few living microbial cells detected at a high throughput by droplet-based ESI-MS
Anal. Chem. 92 (15), 10700 - 10708
full text (doi)
2019 (1)
- Dusny, C., Lohse, M., Reemtsma, T., Schmid, A., Lechtenfeld, O.J. (2019):
Quantifying a biocatalytic product from a few living microbial cells using microfluidic cultivation coupled to FT-ICR-MS
Anal. Chem. 91 (11), 7012 - 7018
full text (doi)
2018 (1)
- Oehling, V., Klaassen, P., Frick, O., Dusny, C., Schmid, A. (2018):
L-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
Biotechnol. Biofuels 11 , art. 231
full text (doi)
2017 (3)
- Delvigne, F., Baert, J., Sassi, H., Fickers, P., Grünberger, A., Dusny, C. (2017):
Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses
Biotechnol. J. 12 (7), art. 1600549
full text (doi) - Fritzsch, F.S.O., Blank, L.M., Dusny, C., Schmid, A. (2017):
Miniaturized octupole cytometry for cell type independent trapping and analysis
Microfluid. Nanofluid. 21 (8), art. 130
full text (doi) - Rosenthal, K., Oehling, V., Dusny, C., Schmid, A. (2017):
Beyond the bulk: disclosing the life of single microbial cells
FEMS Microbiol. Rev. 41 (6), 751 - 780
full text (doi)
2016 (2)
- Dusny, C., Schmid, A. (2016):
The MOX promoter in Hansenula polymorpha is ultrasensitive to glucose-mediated carbon catabolite repression
FEMS Yeast Res. 16 (6), fow067
full text (doi) - Dusny, C., Schmid, A. (2016):
Neue Einblicke in die Welt isolierter Mikroben
Biospektrum 22 (7), 708 - 711
full text (doi)
2015 (4)
- Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., Schmid, A. (2015):
Technical bias of microcultivation environments on single-cell physiology
Lab Chip 15 (8), 1822 - 1834
full text (doi) - Dusny, C., Schmid, A. (2015):
Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes
Environ. Microbiol. 17 (6), 1839 - 1856
full text (doi) - Dusny, C., Schmid, A. (2015):
Challenging biological limits with microfluidic single cell analysis
Microb. Biotechnol. 8 (1), 23 - 25
full text (doi) - Rosenthal, K., Falke, F., Frick, O., Dusny, C., Schmid, A. (2015):
An inert continuous microreactor for the isolation and analysis of a single microbial cell
Micromachines 6 (12), 1836 - 1855
full text (doi)
There are always projects available within our research themes (see below). Projects are interesting for students with a background in biotechnology, biochemical engineering, biochemistry or related.
Microscale analysis of bioconversion in presence of sorbents
Despite the growing interest in the application of sorption-enhanced bioprocesses, such as addition of activated carbon to bioreactors for improved bioproduction or pollutant removal, the mechanisms of sorbent-mediated chemical catchment and subsequent transfer of the reactant to the bacteria are still in the void. This applies, in particular, for technical scenarios where substrate concentrations are below threshold values for growth, maintenance, gene expression, as well as biocatalyst affinity and thus impair the performance of the process. By using microfluidic bioreactors that facilitate stringent environmental control, bacteria can be brought into close proximity or direct physical contact to sorbents loaded with substrate in order to judge on the mode of mass transfer. Based on time-lapse microscopy, growth and gene expression of individual bacterial cells brought in contact with adsorbents with different surface chemistry can be tested.
SYMPOSIUM:
Quantitative Single-Cell Analysis in Bioprocesses
- Sensing, Sampling, Analytics -
Venue: Helmholtz Centre for Environmental Research (UFZ),
KUBUS - UFZ Leipzig, Permoserstr. 15, 04318 Leipzig
Date: 23 - 24 March 2020