Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1002/adsc.201500205 |
Document | Shareable Link |
Title (Primary) | Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms |
Author | Lang, K.; Buehler, K.; Schmid, A. |
Journal | Advanced Synthesis & Catalysis |
Year | 2015 |
Department | SOMA |
Volume | 357 |
Issue | 8 |
Page From | 1919 |
Page To | 1927 |
Language | englisch |
Supplements | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadsc.201500205&file=adsc_201500205_sm_miscellaneous_information.pdf |
Keywords | biofilms; continuous process; fermentation; (S)-3-hydroxyisobutyric acid; Pseudomonas |
UFZ wide themes | MIKAT; RU4; |
Abstract | The chiral building block (S)-3-hydroxyisobutyric acid [(S)-3-HIBA] was produced either by conversion of isobutyric acid or directly from glucose utilizing cells of Pseudomonas taiwanensis VLB120 B83 T7 as catalysts. This strain carries a point mutation in the gene encoding 3-hydroxyisobutyrate dehydrogenase (mmsB), leading to its inactivation. The maximal specific activity in resting-cell biotransformations using isobutyric acid as substrate was 4.9±0.4 U gcdw−1. Overexpression of the 2-ketoisovalerate pathway genes alsS, ilvC, and ilvD, and the introduction of kivd encoding 2-ketoacid decarboxylase resulted in the efficient fermentative synthesis of (S)-3-HIBA directly from glucose. Up to 22 mM (2.3 g L−1) (S)-3-HIBA were produced at 3.7±0.3 U gcdw−1 in repeated batch experiments without observable product degradation. Utilizing a biofilm reactor it was possible to continuously produce up to 6 mM (0.63 g L−1) of (S)-3-hydroxyisobutyric acid with a volumetric productivity of 1.32 mmol(S)-3-HIBA h−1 L−1. Overall, the conversion of isobutyric acid to (S)-3-HIBA was found to be the rate-limiting step, leading to the accumulation of a mixture of (S)-3-hydroxyisobutyric acid and isobutyric acid. This study demonstrates for the first time the production of (S)-3-HIBA from renewable carbon in shake flasks and biofilm reactors and sets the stage for further optimizations towards the efficient production of 3-HIBA and its derivatives in continuous fermentations. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16270 |
Lang, K., Buehler, K., Schmid, A. (2015): Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms Adv. Synth. Catal. 357 (8), 1919 - 1927 |