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Title (Primary) Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms
Author Lang, K.; Buehler, K.; Schmid, A.;
Journal Advanced Synthesis & Catalysis
Year 2015
Department SOMA;
Volume 357
Issue 8
Language englisch;
POF III (all) R61;
Keywords biofilms; continuous process; fermentation; (S)-3-hydroxyisobutyric acid; Pseudomonas
UFZ wide themes MIKAT; RU4;
Abstract

The chiral building block (S)-3-hydroxyisobutyric acid [(S)-3-HIBA] was produced either by conversion of isobutyric acid or directly from glucose utilizing cells of Pseudomonas taiwanensis VLB120 B83 T7 as catalysts. This strain carries a point mutation in the gene encoding 3-hydroxyisobutyrate dehydrogenase (mmsB), leading to its inactivation. The maximal specific activity in resting-cell biotransformations using isobutyric acid as substrate was 4.9±0.4 U gcdw−1. Overexpression of the 2-ketoisovalerate pathway genes alsS, ilvC, and ilvD, and the introduction of kivd encoding 2-ketoacid decarboxylase resulted in the efficient fermentative synthesis of (S)-3-HIBA directly from glucose. Up to 22 mM (2.3 g L−1) (S)-3-HIBA were produced at 3.7±0.3 U gcdw−1 in repeated batch experiments without observable product degradation. Utilizing a biofilm reactor it was possible to continuously produce up to 6 mM (0.63 g L−1) of (S)-3-hydroxyisobutyric acid with a volumetric productivity of 1.32 mmol(S)-3-HIBA h−1L−1. Overall, the conversion of isobutyric acid to (S)-3-HIBA was found to be the rate-limiting step, leading to the accumulation of a mixture of (S)-3-hydroxyisobutyric acid and isobutyric acid. This study demonstrates for the first time the production of (S)-3-HIBA from renewable carbon in shake flasks and biofilm reactors and sets the stage for further optimizations towards the efficient production of 3-HIBA and its derivatives in continuous fermentations.

ID 16270
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16270
Lang, K., Buehler, K., Schmid, A. (2015):
Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms
Adv. Synth. Catal. 357 (8), 1919 - 1927