Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1128/msystems.00302-23
Licence creative commons licence
Title (Primary) Spatial organization and proteome of a dual-species cyanobacterial biofilm alter among N2-fixing and non-fixing conditions
Author Bozan, M. ORCID logo ; Schmidt, M.; Musat, N.; Schmid, A.; Adrian, L.; Bühler, K.
Source Titel mSystems
Year 2023
Department ISOBIO; UMB; UBT; SOMA
Volume 8
Issue 3
Page From art. 00302-23
Language englisch
Topic T7 Bioeconomy
Supplements https://journals.asm.org/doi/suppl/10.1128/msystems.00302-23/suppl_file/msystems.00302-23-s0001.tif
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https://journals.asm.org/doi/suppl/10.1128/msystems.00302-23/suppl_file/msystems.00302-23-s0004.docx
https://journals.asm.org/doi/suppl/10.1128/msystems.00302-23/suppl_file/msystems.00302-23-s0004.docx
https://journals.asm.org/doi/suppl/10.1128/msystems.00302-23/suppl_file/msystems.00302-23-s0006.docx
https://journals.asm.org/doi/suppl/10.1128/msystems.00302-23/suppl_file/msystems.00302-23-s0008.xlsx
https://journals.asm.org/doi/suppl/10.1128/msystems.00302-23/suppl_file/msystems.00302-23-s0009.docx
Keywords Tolypothrix; biofilms; microbial interactions; CLSM; cyanobacteria; proteomics
Abstract Many disciplines have become increasingly interested in cyanobacteria, due to their ability to fix CO2 while using water and sunlight as electron and energy sources. Further, several species of cyanobacteria are also capable of fixing molecular nitrogen, making them independent of the addition of nitrate or ammonia. Thereby they hold huge potential as sustainable biocatalysts. Here, we look into a dual-species biofilm consisting of filamentous diazotrophic cyanobacteria Tolypothrix sp. PCC 7712 and heterotrophic bacteria Pseudomonas taiwanensis VLB 120 growing in a capillary biofilm reactor. Such systems have been reported to enable high cell densities continuous process operation. By combining confocal laser scanning and helium-ion microscopy with a proteomics approach, we examined these organisms’ interactions under two nitrogen-feeding strategies: N2-fixing and nitrate assimilation. Not only did Pseudomonas facilitate the biofilm formation by forming a carpet layer on the surface area but also did N2-fixing biofilms show greater attachment to the surface. Pseudomonas proteins related to surface and cell attachments were observed in N2-fixing biofilms in particular. Furthermore, co-localized biofilm cells displayed a resilient response to extra shear forces induced by segmented media/air flows. This study highlights the role of Pseudomonas in the initial attachment process, as well as the effects of different nitrogen-feeding strategies and operation regimes on biofilm composition and growth.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22727
Bozan, M., Schmidt, M., Musat, N., Schmid, A., Adrian, L., Bühler, K. (2023):
Spatial organization and proteome of a dual-species cyanobacterial biofilm alter among N2-fixing and non-fixing conditions
mSystems 8 (3), art. 00302-23