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Title (Primary) Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Author Salamanca, D.; Karande, R.; Schmid, A.; Dobslaw, D.;
Journal Applied Microbiology and Biotechnology
Year 2015
Department SOMA;
Volume 99
Issue 16
Language englisch;
POF III (all) T15;
Keywords Cyclohexane; CYP450; monooxygenase; Cyclohexanol; Cycloalkanes, Biodegradation
UFZ wide themes MIKAT; RU4
Abstract Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols.
ID 16440
Persistent UFZ Identifier
Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897