Publication Details |
Reference Category | Journals |
DOI / URL | link |
Document | Shareable Link |
Title (Primary) | Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 |
Author | Salamanca, D.; Karande, R.; Schmid, A.; Dobslaw, D.; |
Journal | Applied Microbiology and Biotechnology |
Year | 2015 |
Department | SOMA; |
Volume | 99 |
Issue | 16 |
Language | englisch; |
POF III (all) | T15; |
Keywords | Cyclohexane; CYP450; monooxygenase; Cyclohexanol; Cycloalkanes, Biodegradation |
UFZ wide themes | MIKAT; RU4 |
Abstract | Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols. |
ID | 16440 |
Persistent UFZ Identifier | http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16440 |
Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015): Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897 |