Publication Details |
| Reference Category | Journals |
| DOI / URL | link |
| Document | Shareable Link |
| Title (Primary) | Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 |
| Author | Salamanca, D.; Karande, R.; Schmid, A.; Dobslaw, D.; |
| Journal | Applied Microbiology and Biotechnology |
| Year | 2015 |
| Department | SOMA; |
| Volume | 99 |
| Issue | 16 |
| Language | englisch; |
| POF III (all) | T15; |
| Keywords | Cyclohexane; CYP450; monooxygenase; Cyclohexanol; Cycloalkanes, Biodegradation |
| UFZ wide themes | MIKAT; RU4 |
| Abstract | Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols. |
| ID | 16440 |
| Persistent UFZ Identifier | http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16440 |
| Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015): Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897 |
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