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Category Text Publication
Reference Category Journals
DOI 10.1007/s00253-015-6599-9
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Title (Primary) Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Author Salamanca, D.; Karande, R.; Schmid, A.; Dobslaw, D.
Source Titel Applied Microbiology and Biotechnology
Year 2015
Department SOMA
Volume 99
Issue 16
Page From 6889
Page To 6897
Language englisch
Keywords Cyclohexane; CYP450; monooxygenase; Cyclohexanol; Cycloalkanes, Biodegradation
UFZ wide themes MIKAT; RU4
Abstract Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols.
Persistent UFZ Identifier
Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897 10.1007/s00253-015-6599-9