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Title (Primary) Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Author Salamanca, D.; Karande, R.; Schmid, A.; Dobslaw, D.;
Journal Applied Microbiology and Biotechnology
Year 2015
Department SOMA;
Volume 99
Issue 16
Language englisch;
POF III (all) T15;
Keywords Cyclohexane; CYP450; monooxygenase; Cyclohexanol; Cycloalkanes, Biodegradation
UFZ wide themes MIKAT; RU4
Abstract Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols.
ID 16440
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16440
Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897