Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.mex.2019.07.021
Licence creative commons licence
Title (Primary) Mixed-trophies biofilm cultivation in capillary reactors
Author Heuschkel, I.; Hoschek, A.; Schmid, A.; Bühler, B.; Karande, R.; Bühler, K.
Source Titel MethodsX
Year 2019
Department SOMA
Volume 6
Page From 1822
Page To 1831
Language englisch
Keywords Synechocystis sp. PCC 6803; Pseudomonas; mixed-species biofilm; high cell density culture; photosynthesis
UFZ wide themes MIKAT;

The biocatalytic application of photoautotrophic organisms is a promising alternative for the production of biofuels and value-added compounds as they do not rely on carbohydrates as a source of carbon, electrons, and energy. Although the photoautotrophic organisms hold potential for the development of sustainable processes, suitable reactor concepts that allow high cell density (HCD) cultivation of photoautotrophic microorganisms are limited. Such reactors need a high surface to volume ratio to enhance light availability. Furthermore, the accumulation of high oxygen concentrations as a consequence of oxygenic photosynthesis, and its inhibitory effect on cell growth needs to be prevented. Here, we present a method for HCD cultivation of oxygenic phototrophs based on the co-cultivation of different trophies in a biofilm format to avoid high oxygen partial-pressure and attain HCDs of up to 51.8 gBDW L-1 on a lab scale. In this article, we show:

A robust method for mixed trophies biofilm cultivation in capillary reactors

Set-up and operation of a biofilm capillary reactor

A method to quantify oxygen in the continuous biofilm capillary reactor

Persistent UFZ Identifier
Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-trophies biofilm cultivation in capillary reactors
MethodsX 6 , 1822 - 1831 10.1016/j.mex.2019.07.021