Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1016/j.jbiotec.2024.01.001
Licence creative commons licence
Title (Primary) Glycolate as alternative carbon source for Escherichia coli
Author Höhmann, S.M.; Briol, T.A.; Ihle, N.; Frick, O.; Schmid, A.; Bühler, B.
Source Titel Journal of Biotechnology
Year 2024
Volume 381
Page From 76
Page To 85
Language englisch
Topic T7 Bioeconomy
Keywords Alternative carbon source; Glycolic acid; Laboratory adaption; Physiology; Glycolate utilization
Abstract The physiology of different Escherichia coli stains was analyzed for growth with glycolate as a potentially promising sustainable sole source of carbon and energy. Different E. coli strains showed large differences regarding lag phases after provision of glycolate. Whereas E. coli W showed fast adaptation, E. coli BW25113, JM101, and BL21 (DE3) needed extensive time for adaption (up to 30 generations) until the attainable µmax was reached, which, at 30 °C, amounted to 0.20–0.25 h−1 for all strains. The overexpression of genes encoding glycolate degradation did neither overcome the need for adaptation of E. coli BL21 (DE3) nor improve growth of E. coli W. Rather, high level expression of proteins involved in uptake and initial degradation steps had an adverse effect on growth. Overall, the results show a promising capacity of E. coli strains for growth on glycolate.
Persistent UFZ Identifier
Höhmann, S.M., Briol, T.A., Ihle, N., Frick, O., Schmid, A., Bühler, B. (2024):
Glycolate as alternative carbon source for Escherichia coli
J. Biotechnol. 381 , 76 - 85 10.1016/j.jbiotec.2024.01.001