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Title (Primary) Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
Author Hoschek, A.; Bühler, B.; Schmid, A.;
Journal Biotechnology and Bioengineering
Year 2019
Department SOMA;
Volume 116
Issue 8
Language englisch;
POF III (all) R61; T15;
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fbit.27006&file=bit27006-sup-0001-Supplemental_Information_revision.docx
Keywords biotransformation; in situ product recovery; in situ substrate supply; oxygenase; Synechocystis sp. PCC 6803
Abstract Photoautotrophic organisms are promising hosts for biocatalytic oxyfunctionalizations because they supply reduction equivalents as well as O2 via photosynthetic water oxidation. Thus far, research on photosynthesis‐driven bioprocesses mainly focuses on strain development and the proof of principle in small‐scale biocatalytic reaction setups. This study investigates the long‐term applicability of the previously developed cyanobacterial strain Synechocystis sp. PCC 6803_BGT harboring the alkane monooxygenase system AlkBGT catalyzing terminal alkyl group oxyfunctionalization. For the regiospecific ω‐hydroxylation of nonanoic acid methyl ester (NAME), this biocatalyst showed light intensity‐independent hydroxylation activity and substantial hydrolysis of NAME to nonanoic acid. Substrate mass transfer limitation, substrate hydrolysis, as well as reactant toxicity were overcome via in situ substrate supply by means of a two‐liquid phase system. The application of diisononyl phthalate as organic carrier solvent enabled 1.7‐fold increased initial specific activities (5.6 ± 0.1 U/gCDW) and 7.6‐fold increased specific yields on biomass (3.8 ± 0.1 mmolH‐NAME/gCDW) as compared with single aqueous phase biotransformations. Finally, the whole‐cell biotransformation system was successfully scaled from glass tubes to a stirred‐tank photobioreactor. This is the first study reporting the application of the two‐liquid phase concept for efficient phototrophic whole‐cell biocatalysis.
ID 21867
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21867
Hoschek, A., Bühler, B., Schmid, A. (2019):
Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
Biotechnol. Bioeng. 116 (8), 1887 - 1900