Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.meteno.2016.03.005 |
| Title (Primary) | Dynamics of benzoate metabolism in Pseudomonas putida KT2440 |
| Author | Sudarsan, S.; Blank, L.M.; Dietrich, A.; Vielhauer, O.; Takors, R.; Schmid, A.; Reuss, M. |
| Source Titel | Metabolic Engineering Communications |
| Year | 2016 |
| Department | SOMA |
| Volume | 3 |
| Page From | 97 |
| Page To | 110 |
| Language | englisch |
| Keywords | Metabolic modeling; Pseudomonas putida; Aromatic metabolism; Transient response; Intracellular metabolites |
| UFZ wide themes | RU4; |
| Abstract | Soil microorganisms mineralize lignin-derived aromatic carbon sources using oxidative catabolic pathways, such as the β-ketoadipate pathway. Although this aromatic pathway is one of the best-studied pathways in biochemistry, the complete pathway, including its regulation by aromatic carbon sources, has not been integrated into the metabolic network. In particular, information about the in vivo operation (e.g., kinetics and flux capacity) of the pathway is lacking. In this contribution, we use kinetic modeling and thermodynamic analysis to evaluate the in vivo operation of this key aromatic multi-step pathway. The resulting ab initio deterministic model of benzoate degradation via the β-ketoadipate (ortho-cleavage) pathway in Pseudomonas putida KT2440 is presented. The kinetic model includes mechanistic rate expressions for the enzymes and transport processes. The design and experimental validation of the model are driven by data generated from short-term perturbation experiments in a benzoate-limited continuous culture. The results of rigorous modeling of the in vivo dynamics provide strong support for flux regulation by the benzoate transporter and the enzymes forming and cleaving catechol. Revisiting the β-ketoadipate pathway might be valuable for applications in different fields, such as biochemistry and metabolic engineering, that use lignin monomers as a carbon source. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18078 |
| Sudarsan, S., Blank, L.M., Dietrich, A., Vielhauer, O., Takors, R., Schmid, A., Reuss, M. (2016): Dynamics of benzoate metabolism in Pseudomonas putida KT2440 Metab. Eng. Commun. 3 , 97 - 110 10.1016/j.meteno.2016.03.005 |
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