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Title (Primary) Cellular physiology controls photoautotrophic production of 1,2‐propanediol from pools of CO2 and glycogen
Author David, C.; Schmid, A.; Bühler, K.
Journal Biotechnology and Bioengineering
Year 2019
Department SOMA
Volume 116
Issue 4
Page From 882
Page To 892
Language englisch
Keywords 1,2‐propanediol; carbon flux; cyanobacteria; glycogen; methylglyoxal synthase; Synechocystis sp. PCC 6803
UFZ wide themes MIKAT;
Abstract Synechocystis sp. PCC 6803 PG is a cyanobacterial strain capable of synthesizing 1,2‐propanediol from carbon dioxide (CO2) via a heterologous three‐step pathway and a methylglyoxal synthase (MGS) originating from Escherichia coli as an initial enzyme. The production window is restricted to the late growth and stationary phase and is apparently coupled to glycogen turnover. To understand the underlying principle of the carbon partitioning between the Calvin‐Benson‐Bassham (CBB) cycle and glycogen in the context of 1,2‐propanediol production, experiments utilizing 13C labeled CO2 have been conducted. Carbon fluxes and partitioning between biomass, storage compounds, and product have been monitored under permanent illumination as well as under dark conditions. About one‐quarter of the carbon incorporated into 1,2‐propanediol originated from glycogen, while the rest was derived from CO2 fixed in the CBB cycle during product formation. Furthermore, 1,2‐propanediol synthesis was depending on the availability of photosynthetic active radiation and glycogen catabolism. We postulate that the regulation of the MGS from E. coli conflicts with the heterologous reactions leading to 1,2‐propanediol in Synechocystis sp. PCC 6803 PG. Additionally, homology comparison of the genomic sequence to genes encoding for the methylglyoxal bypass in E. coli suggested the existence of such a pathway also in Synechocystis sp. PCC 6803. These findings are critical for all heterologous pathways coupled to the CBB cycle intermediate dihydroxyacetone phosphate via a MGS and reveal possible engineering targets for rational strain optimization.
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David, C., Schmid, A., Bühler, K. (2019):
Cellular physiology controls photoautotrophic production of 1,2‐propanediol from pools of CO2 and glycogen
Biotechnol. Bioeng. 116 (4), 882 - 892