Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/bit.27784
Licence creative commons licence
Title (Primary) Technical-scale biophotovoltaics for long-term photo-current generation from Synechocystis sp. PCC6803
Author Lai, B. ORCID logo ; Schneider, H.; Tschörtner, J.; Schmid, A.; Krömer, J.O.
Source Titel Biotechnology and Bioengineering
Year 2021
Department SOMA
Volume 118
Issue 7
Page From 2637
Page To 2648
Language englisch
Topic T7 Bioeconomy
Keywords Bioelectrochemical systems; cyanobacteria; microbial electrochemical technology; BG11 medium; system design
Abstract A carbon‐free energy supply is essential to sustain our future. Biophotovoltaics (BPV) provides a promising solution for hydrogen supply by directly coupling light‐driven water splitting to hydrogen formation using oxygenic photoautotrophic cyanobacteria. However, BPV is currently limited by its low photon‐to‐current efficiency, and current experimental setups at a miniaturized scale hinder the rational investigation of the process and thus system optimization. In this paper, we developed and optimized a new technical‐scale (~250ml working volume) BPV platform with defined and controllable operating parameters. Factors that interfered with reproducible and stable current output signals were identified and adapted. We found that the classical BG11 medium, used for the cultivation of cyanobacteria and also in many BPV studies, caused severe interferences in the bioelectrochemical experiments. An optimized nBG11 medium guaranteed a low and stable background current in the BPV reactor, regardless of the presence of light and/or mediators. As proof‐of‐principle, a very high long‐term light‐dependent current output (peak current of over 20 µA) was demonstrated in the new set‐up over 12 days with living Synechocystis sp PCC6803 cells and validated with appropriate controls. These results report the first reliable BPV platform generating reproducible photocurrent while still allowing quantitative investigation, rational optimization, and scale‐up of BPV processes.
Persistent UFZ Identifier
Lai, B., Schneider, H., Tschörtner, J., Schmid, A., Krömer, J.O. (2021):
Technical-scale biophotovoltaics for long-term photo-current generation from Synechocystis sp. PCC6803
Biotechnol. Bioeng. 118 (7), 2637 - 2648 10.1002/bit.27784