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Title (Primary) Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization
Author Hoschek, A.; Bühler, B.; Schmid, A.;
Journal Angewandte Chemie-International Edition
Year 2017
Department SOMA;
Volume 56
Issue 47
Language englisch;
POF III (all) R61;
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fanie.201706886&attachmentId=213476525
Keywords oxygen mass transfer; oxyfunctionalization
UFZ wide themes RU4;
Abstract Gas-liquid mass transfer of gaseous reactants is a major limitation for high space-time yields, especially for O₂-dependent (bio)catalytic reactions in aqueous solutions. Oxygenic photosynthesis was used for homogeneous O₂-supply via in situ generation in the liquid phase to overcome gas-liquid mass transfer limitations. The phototrophic cyanobacterium Synechocystis sp. PCC6803 was engineered to synthesize alkane monooxygenase AlkBGT, originating from Pseudomonas putida GPo1. With light, but without external addition of O₂, the chemo- and regioselective hydroxylation of nonanoic acid methyl ester to ω-hydroxynonanoic acid methyl ester was driven by O₂ generated via photosynthetic water oxidation. Photosynthesis also delivered the necessary reduction equivalents regenerating Fe2+ in AlkB for oxygen transfer to the terminal methyl group. The in situ coupling of oxygenic photosynthesis to O₂-transferring enzymes now allows the design of fast hydrocarbon oxyfunctionalization reactions.
ID 19280
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19280
Hoschek, A., Bühler, B., Schmid, A. (2017):
Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization
Angew. Chem.-Int. Edit. 56 (47), 15146 - 15149