Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/anie.201706886 |
| Title (Primary) | Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization |
| Author | Hoschek, A.; Bühler, B.; Schmid, A. |
| Source Titel | Angewandte Chemie-International Edition |
| Year | 2017 |
| Department | SOMA |
| Volume | 56 |
| Issue | 47 |
| Page From | 15146 |
| Page To | 15149 |
| Language | englisch |
| Supplements | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fanie.201706886&attachmentId=213476525 |
| Keywords | oxygen mass transfer; oxyfunctionalization |
| UFZ wide themes | RU4; |
| Abstract | Gas-liquid mass transfer of gaseous reactants is a major limitation for high space-time yields, especially for O₂-dependent (bio)catalytic reactions in aqueous solutions. Oxygenic photosynthesis was used for homogeneous O₂-supply via in situ generation in the liquid phase to overcome gas-liquid mass transfer limitations. The phototrophic cyanobacterium Synechocystis sp. PCC6803 was engineered to synthesize alkane monooxygenase AlkBGT, originating from Pseudomonas putida GPo1. With light, but without external addition of O₂, the chemo- and regioselective hydroxylation of nonanoic acid methyl ester to ω-hydroxynonanoic acid methyl ester was driven by O₂ generated via photosynthetic water oxidation. Photosynthesis also delivered the necessary reduction equivalents regenerating Fe2+ in AlkB for oxygen transfer to the terminal methyl group. The in situ coupling of oxygenic photosynthesis to O₂-transferring enzymes now allows the design of fast hydrocarbon oxyfunctionalization reactions. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19280 |
| Hoschek, A., Bühler, B., Schmid, A. (2017): Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization Angew. Chem.-Int. Edit. 56 (47), 15146 - 15149 10.1002/anie.201706886 |
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