Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/bit.26469 |
| Document | Shareable Link |
| Title (Primary) | Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120 |
| Author | Karande, R.; Salamanca, D.; Schmid, A.; Buehler, K. |
| Source Titel | Biotechnology and Bioengineering |
| Year | 2018 |
| Department | SOMA |
| Volume | 115 |
| Issue | 2 |
| Page From | 312 |
| Page To | 320 |
| Language | englisch |
| Supplements | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fbit.26469&attachmentId=2172021390 |
| Keywords | cycloalkanes; in-vivo cascade; lactones; Pseudomonas; whole-cell biocatalysis |
| UFZ wide themes | MIKAT; RU4; |
| Abstract | Chemical synthesis of lactones from cycloalkanes is a multi-step process challenged by limitations in reaction efficiency (conversion and yield), atom economy (by-products) and environmental performance. A heterologous pathway comprising novel enzymes with compatible kinetics was designed in Pseudomonas taiwanensis VLB120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. The respective pathway included cytochrome P450 monooxygenase (CHX), cyclohexanol dehydrogenase (CDH), and cyclohexanone monooxygenase (CHXON) from Acidovorax sp. CHX100. Resting (non-growing) cells of the recombinant host P. taiwanensis VLB120 converted cyclohexane, cyclohexanol, and cyclohexanone to ϵ-caprolactone at 22, 80–100, and 170 U gCDW−1, respectively. Cyclohexane (5 mM) was completely converted with a selectivity of 65% for ϵ-caprolactone formation in 2 hr without accumulation of intermediate products. Promiscuity of the whole-cell biocatalyst gave access to analogous lactones from cyclooctane and cyclodecane. A total product concentration of 2.3 g L−1 and a total turnover number of 36,720 was achieved over 5 hr with a biocatalyst concentration of 6.8 gCDW L−1. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19506 |
| Karande, R., Salamanca, D., Schmid, A., Buehler, K. (2018): Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120 Biotechnol. Bioeng. 115 (2), 312 - 320 10.1002/bit.26469 |
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