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Title (Primary) Directed reaction engineering boosts succinate formation of Synechocystis sp. PCC 6803_Δsll1625
Author Mock, M.; Schmid, A.; Bühler, K.
Journal Biotechnology Journal
Year 2020
Department SOMA
Volume 15
Issue 11
Page From art. 2000127
Language englisch
Keywords cyanobacteria; photoautotroph; photobiotechnology; succinate; Synechocystis
Abstract It is known that Synechocystis sp. PCC 6803 carrying a partial deletion of the succinate dehydrogenase (Synechocystis_∆sll1625) secretes succinate during aerobic cultivation with continuous illumination and in the presence of CO2. Maximal succinate titers of 2 mM (236 mg L−1) were reported [1]. CO2 was identified as a crucial parameter for product formation, however, a detailed characterization of different cultivation conditions is still missing.

Here we focused on further reaction engineering to improve the photoautotrophic production of succinate using Synechocystis_∆sll1625. Therefore the impact of light availability, illumination regimes, nutrient availability and external pH on product formation were investigated. Results obtained in this study revealed the importance of these parameters on the formation of succinate and cultivation with light/dark cycles increased the succinate concentration to 3 mM (354 mg L−1) after 28 days of cultivation. Furthermore cultivation in unbuffered medium under ambient CO2 conditions even doubled the final succinate titer to 4 mM (472 mg L−1) after 28 days.

Taking biomass concentrations into account, a maximal yield of succinate on biomass of 215 mgSucc gCDW−1 was achieved, which is the highest so far reported for the production of succinate utilizing Synechocystis as host organism.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23562
Mock, M., Schmid, A., Bühler, K. (2020):
Directed reaction engineering boosts succinate formation of Synechocystis sp. PCC 6803_Δsll1625
Biotechnol. J. 15 (11), art. 2000127