Education

Positions

Index:

2015    (4)                  2007    (1)     
2014    (6)                  2006    (3)
2013    (7)                  2004    (1)
2012    (8)                  2003    (2)
2011    (5)                  2002    (2)
2010    (4)                  2000    (1)
2008    (4)                  1999    (1)

2015 (4)

• Looße C., Galozzi S., Debor L., Julsing M. K., Bühler B., Schmid A., Barkovits K., Müller T. and Marcus K. (2015)
  Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples.
  EuPA Open Proteomics, 7:20-26, DOI-Link

• Collins, J., Grund, M., Brandenbusch, C., Sadowski, G., Schmid, A., Bühler, B., (2015):The dynamic influence of cells on the formation of stable emulsions in organic–aqueous biotransformations
  J. Ind. Microbiol. Biotechnol. 42 (7), 1011 - 1026
  

• Falcioni F., Bühler B. and Schmid A. (2015)
  Efficient hydroxyproline production from glucose in minimal media by Corynebacterium glutamicum.
  Biotechnology and Bioengineering, 112(2):322-330, DOI-Link

• Brandenbusch C., Collins J., Glonke S., Hoffrogge R., Grunwald K., Bühler B., Schmid A. and Sadowski G. (2015)
  Process boundaries of irreversible scCO2-assisted phase separation in biphasic whole-cell biocatalysis.
  Biotechnology and Bioengineering, 112(11):2316-2323, DOI-Link
  
  

2014 (6)

• Jahn M., Vorpahl C., Türkowsky D., Lindmeyer M., Bühler B., Harms H. and Müller S. (2014)
  Accurate determination of plasmid copy number on the single cell level using droplet digital PCR.
  Analytical Chemistry, 86(12):5969–5976, DOI-Link
  
  

• Jahn M., Vorpahl C., Türkowsky D., Lindmeyer M., Bühler B., Harms H. and Müller S. (2014)
  Population heterogeneity in Pseudomonas putida analyzed on the single cell level using proteomics and digital PCR
  New Biotechnology, 31: S58-S59
  

• Volmer J., Neumann C., Bühler B. and Schmid A. (2014)
  Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity.
  Applied and Environmental Microbiology, 80(20):6539-6548, DOI-Link

• Willrodt C., David C., Cornelissen S., Bühler B., Julsing M. K. and Schmid A. (2014)
  Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.
  Biotechnology Journal, 9(8):1000–1012, DOI-Link

• Schrewe M., Julsing M. K., Lange K., Czarnotta E, Schmid A. and Bühler B. (2014)
  Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.
  Biotechnology and Bioengineering, 111(9):1820-1830, DOI-Link

• Ladkau N., Schmid A. and Bühler B. (2014)
  The microbial cell - functional unit for energy-dependent multistep biocatalysis.
  Current Opinion in Biotechnology, 30:178-189, DOI-Link
  
  

2013 (7)

• Steen A., Ütkür F.Ö., Borrero-de Acuna J. M., Bunk B., Roselius L., Bühler B., Jahn D. and Schobert M. (2013)
  Construction and characterization of nitrate and nitrite respiring Pseudomonas putida KT2440 strains for anoxic biotechnical applications.
  Journal of Biotechnology, 163(2):155-165
  

• Schrewe M., Ladkau N., Bühler B. and Schmid A. (2013)
  Direct terminal alkylamino-functionalization via multistep biocatalysis in one recombinant whole-cell catalyst.
  Advanced Synthesis and Catalysis, 355(9):1693-1697

• Falcioni F., Blank L. M., Frick O., Karau A., Bühler B. and Schmid A. (2013)
  Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.
  Applied and Environmental Microbiology, 79(9):3091-3100

• Jahn M., Seifert J., von Bergen M., Schmid A., Bühler B. and Müller S. (2013)
  Subpopulation-proteomics in prokaryotic populations.
  Current Opinion in Biotechnology, 24(1):79-87

• Kuhn D., Fritzsch F. S. O., Zhang X., Wendisch V. F., Blank L. M., Bühler B. and Schmid A. (2013)
  Subtoxic product levels limit the epoxidation capacity of recombinant E. coli by increasing microbial energy demands.
  Journal of Biotechnology, 163(2):194-203, PubMed

• Schrewe M.*, Julsing M. K.*, Bühler B. and Schmid A. (2013)
  Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.
  Chemical Society Reviews, 42(15):6346 - 6377
  * These authors contributed equally to this work.

• Cornelissen S., Julsing M. K., Volmer J., Riechert O., Schmid A. and Bühler B. (2013)
  Whole-cell-based CYP153A6-catalyzed (S)-limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL.
  Biotechnology and Bioengineering, 110(5):1282-1292
  
  

2012 (8)

• Cornelissen S., Julsing M. K., Schmid A. and Bühler B. (2012)
  Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis.
  Journal of Industrial Microbiology and Biotechnology, 39(2):275-287

• Ütkür F.Ö., Tran T.-T., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
  Integrating organic-aqueous biocatalysis and product recovery using quinaldine 4-oxidase in living P. putida KT2440 (pKP1).
  Journal of Industrial Microbiology & Biotechnology, 39(7):1049-1059

• Kuhn D., Bühler B. and Schmid A. (2012)
  Production host selection for asymmetric styrene epoxidation: Escherichia coli vs. solvent tolerant Pseudomonas.
  Journal of Industrial Microbiology and Biotechnology, 39(8):1125-1133

• Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
  Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
  Biotechnology and Bioengineering, 109(5):1109-1119

• Braun A., Geier M., Bühler B., Schmid A., Mauersberger S. and Glieder A. (2012)
  Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.
  Microbial Cell Factories, 11:106

• Kuhn D., Julsing M. K., Heinzle E. and Bühler B. (2012)
  Systematic optimization of a biocatalytic two-liquid phase oxyfunctionalization process guided by ecological andeconomic assessment.
  Green Chemistry, 14(3):645 - 653

• Julsing M. K.*, Schrewe M.*, Cornelissen S., Hermann I., Schmid A. and Bühler B. (2012)
  The outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in E. coli.
  Applied and Environmental Microbiology, 78(16):5724-5733
  * These authors contributed equally to this work.

• Escapa I. F., Garcia J. L., Bühler B., Blank L. M. and Prieto M.A. (2012)
  The polyhydroxyalkanoate metabolism controls carbon and energy spillage in Pseudomonas putida.
  Environmental Microbiology, 14(4):1049-1063
  
  

2011 (5)

• Cornelissen S., Liu S, Deshmukh A.T., Schmid A. and Bühler B. (2011)
  Cell physiology can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.
  Journal of Industrial Microbiology and Biotechnology, 38:1359-1370

• Ladkau N., Hermann I., Bühler B. and Schmid A. (2011)
  Enzyme catalyzed laurolactam synthesis via intramolecular amide bond formation in aqueous solution.
  Advanced Synthesis and Catalysis, 353(13):2501-2510

• Hollmann F., Arends I. W. C. E, Bühler K., Schallmey A. and Bühler B. (2011)
  Enzyme-mediated oxidations for the chemist.
  Green Chemistry, 13:226-265

• Schrewe M., Magnusson A., Willrodt C., Bühler B. and Schmid A. (2011)
  Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis.
  Advanced Synthesis and Catalysis, 353:3485 – 3495

• Ütkür F.Ö., Gaykawad S., Bühler B. and Schmid A. (2011)
  Regioselective aromatic hydroxylation of quinaldine with water using quinaldine 4-oxidase in recombinant Pseudomonas putida.
  Journal of Industrial Microbiology and Biotechnology, 38(8):1067-1077

2010 (4)

• Brandenbusch C., Bühler B., Hoffmann P., Sadowski G. and Schmid A. (2010)
  Efficient phase separation and product recovery in organic-aqueous bioprocessing using supercritical carbon dioxide.
  Biotechnology and Bioengineering, 107(4):642-651

• Kuhn D., Kholiq A., Heinzle E., Bühler B. and Schmid A. (2010)
  Intensification and economic and ecological assessment of a biocatalytic oxyfunctionalization process.
  Green Chemistry, 12(5):815-827

• Blank L. M., Ebert B. E., Bühler K. and Bühler B. (2010)
  Redox biocatalysis and metabolism: molecular mechanisms and metabolic network analysis.
  Antioxidants & Redox Signaling, 13(3):349-394, PubMed

• Kuhn D., Blank L. M., Schmid A. and Bühler B. (2010)
  Systems biotechnology - rational biocatalyst and bioprocess design.
  Engineering in Life Sciences, 10(5):384–397
  
  

2008 (4)

• Julsing M. K., Cornelissen S., Bühler B. and Schmid A. (2008)
  Heme-iron oxygenases: powerful industrial biocatalysts?
  Current Opinion in Chemical Biology, 12(2):177-186, PubMed

• Blank L. M., Ebert B. E., Bühler B. and Schmid A. (2008)
  Metabolic capacity estimation of Escherichia coli as platform for redox biocatalysis: Constraint based modeling and experimental verification.
  Biotechnology and Bioengineering, 100(6):1050-1065, PubMed

• Blank L. M., Ionidis G., Ebert B. E., Bühler B. and Schmid A. (2008)
  Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.
  FEBS Journal, 275(20):5173-5190, PubMed

• Bühler B., Park J. B., Blank L. M. and Schmid A. (2008)
  NADH availability limits asymmetric biocatalytic epoxidation in growing recombinant Escherichia coli.
  Applied and Environmental Microbiology, 74(5):1436-1446, PubMed
  
  

2007 (1)

• Park J. B., Bühler B., Panke S., Witholt B. and Schmid A. (2007)
  Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent tolerant Pseudomonas sp. strain VLB120ΔC.
  Biotechnology and Bioengineering, 98(6):1219-1229, PubMed
  
  

2006 (3)

• Bühler B., Straathof A. J. J., Witholt B. and Schmid A. (2006)
  Analysis of two-liquid-phase multistep biooxidation based on a process model: Indications for biological energy shortage.
  Organic Process Research and Development, 10(3):628-643

• Meyer D., Bühler B. and Schmid A. (2006)
  Process and catalyst design objectives for specific redox biocatalysis.
  Advances in Applied Microbiology, 59:53-91, PubMed

• Park J. B.*, Bühler B.*, Habicher T., Hauer B., Panke S., Witholt B. and Schmid A. (2006)
  The efficiency of recombinant Escherichia coli as biocatalyst for stereospecific epoxidation.
  Biotechnology and Bioengineering, 95(3):501-512, PubMed
  * These authors contributed equally to this work.
  
  

2004 (1)

• Bühler B. and Schmid A. (2004)
  Process implementation aspects for biocatalytic hydrocarbon oxyfunctionalisation.
  Journal of Biotechnology, 113(1-3):183-210, PubMed
  
  

2003 (2)

• Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
  Chemical biotechnology for the specific oxyfunctionalization of hydrocarbons an a technical scale.
  Biotechnology and Bioengineering, 82(7):833-842, PubMed

• Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
  Use of the two-liquid phase concept to exploit kinetically controlled multistep biocatalysis.
  Biotechnology and Bioengineering, 81(6):683-694, PubMed
  
  

2002 (2)

• Bühler B., Witholt B., Hauer B. and Schmid A. (2002)
  Characterization and application of xylene monooxygenase for multistep biocatalysis.
  Applied and Environmental Microbiology, 68(2):560-568, PubMed

• Schmid A., Hollmann F., Park J. B. and Bühler B. (2002)
  The use of enzymes in the chemical industry in Europe.
  Current Opinion in Biotechnology, 13(4):359-366, PubMed
  
  

2000 (1)

• Bühler B., Schmid A., Hauer B. and Witholt B. (2000)
  Xylene monooxygenase catalizes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101.
  Journal of Biological Chemistry, 275(14):10085-100092, PubMed
  
  

1999 (1)

• Prieto M.A., Bühler B., Jung K., Witholt B. and Kessler B. (1999)
  PhaF, a polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1 involved in the regulatory expression system of pha genes.
  Journal of Bacteriology, 181(3):858-868, PubMed
  

2017

• Koch R., Klaffl S., Spelberg M., Tischler D., Schlomann M., Schlotissek A., Heine T., Bühler B., Schmid A., Willrodt C.
  Enantionselective enzymatic sulfoxidation of chiral arylsufides.
  WO 2018/206823 / PCT/EP2018/068978
  Priority Data: 17202022.4 16.11.2017 EP

• Schmid A., Hoschek A., and Bühler B.
  Verfahren zur bioreaktiven Extraktion erzeugten Sauerstoffs aus einem Reaktionsraum, sowie Verwendung von phototrophen Mikroorganismen bei der Gewinnung von Wasserstoff
  Germany, DE 10 2017 104 648 A1, filed
  International, PCT/EP2018/055449, filed

2013

• Sadowski G., Brandenbusch C., Collins J. and Bühler B. (2013)
  Applied catastrophic phase inversion.
  Europe, EP 13 005 234.3, priority: 6.11.2013, filed
  
  

2011

• Schmid A., Bühler B., Ladkau N. and Hermann I. (2011)
  Method for omega-aminocarboxylic acid condensation.
  Europe, WO/2012/156151 / PCT/EP2012/056251 /priority: 11004029.2 16.05.2011 EP
  
  

2010

• Pötter M., Schmid A., Bühler B., Hennemann H.-G., Julsing M. K., Schaffer S., Haas T., Schrewe M., Cornelissen S., Roos M. and Häger H. (2010)
  Biocatalytic oxidation process with alkL-gene product.
  WO 2011/131420 / PCT/EP2011/053834 /priority: 10 2010 015 807.0 20.04.2010 DE
  

2007

• Sadowski G., Schmid A., Brandenbusch C., Bühler B. and Görnert M. (2007)
  Method for processing coalescence-inhibited emulsions from whole-cell biotransformations with compressed or supercritical gases.
  WO/2009/012754 / EP2170481 / DE 10 2007 034 258.8 / US 8,431,358 B2

• Karau A., Sieber V., Haas T., Häger H., Grammann K., Bühler B., Blank L. M. and Schmid A. (2007)
  Omega-amino carboxylic acids, omega-amino carboxylic acid esters, or recombinant cells which produce lactams thereof.
  WO/2009/077461 / PCT/EP2008/067447 / priority: 102007060705.0 17.12.2007 DE
  

2001

• Schmid A., Hauer B., Bühler B. and Witholt B. (2001)
  Method for the oxidation of aromatic compunds.
  WO/2002/081718 / PCT/EP2002/003803 / priority: 101 17 359.8 06.04.2001 DE
  

1999

• Schmid A., Bühler B., Hauer B. and Witholt B. (1999)
  Microbiological method for producing aromatic aldehydes and/or carboxylic acids.
  WO/2001/031047 / PCT/EP2000/010552 / priority: 199 51 768.1 27.10.1999 DE
  

2012

• Hollmann F., Bühler K. and Bühler B. (2012)
  Oxidation of Alcohols, Aldehydes, and Acids.
  in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
  Wiley-VCH, Weinheim, Germany, Vol. 3:1325-1437, ISBN: 978-3-527-32547-4

• Bühler B., Bühler K. and Hollmann F. (2012)
  Oxyfunctionalization of C-C-Multiple Bonds.
  in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
  Wiley-VCH, Weinheim, Germany, Vol. 3:1269-1324, ISBN: 978-3-527-32547-4

• Ütkür F.Ö., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
  Regioselective Aromatic Hydroxylation of Quinaldine Using Living Pseudomonas putida Cells Containing Quinaldine 4-oxidase.
  in John Whitthal and Peter Sutton Practical Methods in Biocatalysis and Biotransformations 2.
  John Wiley & Sons Ltd, Chichester, UK:153-157, ISBN: 978-1-119-99139-7
  

2011

• Brandenbusch C., Bühler B., Sadowski G. and Schmid A. (2011)
  Biokatalytische Epoxidierung von Styrol zu enantiomerenreinem (S)-Styroloxid.
  in Chmiel H. Bioprozesstechnik.
  Spektrum Akademischer Verlag, Heidelberg, Germany:486-488, ISBN: 978-3-8274-2476-1

• Ütkür F.Ö. and Bühler B. (2011)
  Molybdenum-Containing Enzymes and their Applications.
  in Ortiz M. and Herrera T. (eds.) Molybdenum: Characteristics, Production and Applications.
  Nova Science Publishers, Inc., Hauppauge, NY, USA:117-146, ISBN: 978-1-61470-504-8
  

2009

• Bühler B., Blank L. M., Ebert B. E., Bühler K. and Schmid A. (2009)
  Energy and cofactor issues in fermentation and oxyfunctionalisation processes.
  in Smolke C. D. The metabolic pathway engineering handbook.
  CRC Press, Boca Raton, Florida, USA, Vol. 2:21 1-32, ISBN: 978-1-4200-7765-0
  

2008

• Eissen M., Geisler G., Bühler B., Fischer C., Hungerbuehler K., Schmid A. and Carreira E. M. (2008)
  Mass Balances and Life Cycle Assessment.
  in Lapkin A. and Constable D. Green Chemistry Metrics: Measuring and Monitoring Sustainable Processes.
  John Wiley & Sons Ltd, Chichester, West Sussex, UK:200-227
  

2005

• Bühler B. and Schmid A. (2005)
  Selective enzymatic hydroxylations.
  in Dyker G. Handbook of C-H transformations.
  Wiley-VCH, Weinheim, Germany, Vol. 2:516-529
  

2003

• Bühler B. (2003)
  PhD Thesis: Specific oxyfunctionalization of pseudocumene by multistep biocatalysis - mechanism and scale up.
  Swiss Federal Institute of Technology (ETH), Zurich, Switzerland
  

2002

• Hollmann F., Bühler B. and Schmid A. (2002)
  Oxidation of alcohols, phenols, aldehydes, acids and C-N bonds.
  in Drauz K. and Waldmann H. Enzyme catalysis in organic synthesis.
  Wiley-VCH, Weinheim, Germany, Vol. III:1108-1202, 1245-1261,ISBN: 3-527-29949-1