Publication Details

Category Text Publication
Reference Category Journals
DOI 10.1002/cbic.202300475
Licence creative commons licence
Title (Primary) Enlighting electron routes in oxyfunctionalizing Synechocystis sp. PCC 6803
Author Tüllinghoff, A.; Toepel, J.; Bühler, B.
Source Titel Chembiochem
Year 2024
Department MIBITECH
Volume 25
Issue 6
Page From e202300475
Language englisch
Topic T7 Bioeconomy
Supplements https://chemistry-europe.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fcbic.202300475&file=cbic202300475-s1-supporting_information_Tuellinghoff.pdf
Keywords light-driven biocatalysis; hydroxylation; ferredoxin; photosynthesis; electron transfer
Abstract Phototrophic microorganisms, like cyanobacteria, are gaining attention as host organisms for biocatalytic processes with light as energy source and water as electron source. Redox enzymes, especially oxygenases, can profit from in-situ supply of co-substrates, i.e., reduction equivalents and O2, by the photosynthetic light reaction. The electron transfer downstream of PS I to heterologous electron consuming enzymes in principle can involve NADPH, NADH, and/or ferredoxin, whereas most direct and efficient transfer is desirable. Here, we use the model organism Synechocystis sp. PCC 6803 to investigate, to what extent host and/or heterologous constituents are involved in electron transfer to a heterologous Cytochrome P450 monooxygenase from Acidovorax sp. CHX100. Interestingly, in this highly active light-fueled cycloalkane hydroxylating biocatalyst, host-intrinsic enzymes were found capable of completely substituting the function of the Acidovorax ferredoxin reductase. To a certain extent (20%), this also was true for the Acidovorax ferredoxin. These results indicate the presence of a versatile set of electron carriers in cyanobacteria, enabling efficient and direct coupling of electron consuming reactions to photosynthetic water oxidation. This will both simplify and promote the use of phototrophic microorganisms for sustainable production processes.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28298
Tüllinghoff, A., Toepel, J., Bühler, B. (2024):
Enlighting electron routes in oxyfunctionalizing Synechocystis sp. PCC 6803
ChemBioChem 25 (6), e202300475 10.1002/cbic.202300475