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Research Focus:

Research in the Applied Biocatalysis group focusses on whole-cell biocatalysis and aims at the development of efficient and stable bioprocesses via an integrated biocatalyst and reaction engineering approach. Specifically, redox biotransformations (oxygenases, dehydrogenases), in vivo cascades / orthogonal pathways, and fermentative approaches are targeted via enzyme, metabolic, reaction, and process engineering to develop and apply engineered microbial cells for the eco-efficient production of bulk and fine chemicals as well as fuels and bioactives from renewable as well as fossil resources. The renewables considered include CO2 and sunlight and thus the exploitation of photosynthesis. Cyanobacteria, E. coli, and Pseudomonads constitute the host strains mainly employed. Following a systems biotechnology approach, special emphasis lies on biocatalyst and process efficiency with the ultimate goal to develop environment-friendly and industrially feasible processes via rational biocatalyst and process engineering.


Index:

2019 (7)
2018 (1)
2017 (6)
2016 (2)
2015 (5)

You could use our publication index for further requests.

2019 (7)

  • Grund, M., Jakob, T., Wilhelm, C., Bühler, B., Schmid, A. (2019):
    Electron balancing under different sink conditions reveals positive effects on photon efficiency and metabolic activity of Synechocystis sp. PCC 6803
    Biotechnol. Biofuels 12 , art. 43
    full text (url)
  • Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
    Data on mixed trophies biofilm for continuous cyclohexane oxidation to cyclohexanol using Synechocystis sp. PCC 6803
    Data in Brief
    full text (url)
  • Hoschek, A., Bühler, B., Schmid, A. (2019):
    Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
    Biotechnol. Bioeng.
    full text (url)
  • Hoschek, A., Heuschkel, I., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
    Mixed-species biofilms for high-cell-density application of Synechocystis sp. PCC 6803 in capillary reactors for continuous cyclohexane oxidation to cyclohexanol
    Bioresour. Technol. 282 , 171 - 178
    full text (url)
  • Hoschek, A., Toepel, J., Hochkeppel, A., Karande, R., Bühler, B., Schmid, A. (2019):
    Light‐dependent and aeration‐independent gram‐scale hydroxylation of cyclohexane to cyclohexanol by CYP450 harboring Synechocystis sp. PCC 6803
    J. Biotechnol.
    full text (url)
  • Ütkür, F.Ö., Schmid, A., Bühler, B. (2019):
    Anaerobic C-H oxyfunctionalization – coupling of nitrate reduction and quinoline hydroxylation in recombinant Pseudomonas putida
    Biotechnology Journal
    full text (url)
  • Volmer, J., Lindmeyer, M., Seipp, J., Schmid, A., Bühler, B. (2019):
    Constitutively solvent-tolerant Pseudomonas taiwanensis VLB120ΔCΔttgV supports particularly high styrene epoxidation activities when grown under glucose excess conditions
    Biotechnol. Bioeng. 116 (5), 1089 - 1101
    full text (url)
to index

2018 (1)

  • Hoschek, A., Schmid, A., Bühler, B. (2018):
    In situ O2 generation for biocatalytic oxyfunctionalization reactions
    ChemCatChem 10 (23), 5366 - 5371
    full text (url)
to index

2017 (6)

  • Hoschek, A., Bühler, B., Schmid, A. (2017):
    Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization
    Angew. Chem.-Int. Edit. 56 (47), 15146 - 15149
    full text (url)
  • Kadisch, M., Julsing, M.K., Schrewe, M., Jehmlich, N., Scheer, B., von Bergen, M., Schmid, A., Bühler, B. (2017):
    Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance
    Biotechnol. Bioeng. 114 (4), 874 - 884
    full text (url)
  • Kadisch, M., Schmid, A., Bühler, B. (2017):
    Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing
    J. Ind. Microbiol. Biotechnol. 44 (3), 339 - 351
    full text (url)
  • Kadisch, M., Willrodt, C., Hillen, M., Bühler, B., Schmid, A. (2017):
    Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts
    Biotechnology Journal 12 (8), art. 1600170
    full text (url)
  • Theodosiou, E., Breisch, M., Julsing, M.K., Falcioni, F., Bühler, B., Schmid, A. (2017):
    An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli
    Biotechnol. Bioeng. 114 (7), 1511 - 1520
    final draft (pdf)
  • Volmer, J., Schmid, A., Bühler, B. (2017):
    The application of constitutively solvent-tolerant P. taiwanensis VLB120ΔCΔttgV for stereospecific epoxidation of toxic styrene alleviates carrier solvent use
    Biotechnology Journal 12 (7), art. 1600558
    final draft (pdf)
to index

2016 (2)

  • Ladkau, N., Assmann, M., Schrewe, M., Julsing, M.K., Schmid, A., Bühler, B. (2016):
    Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli
    Metab. Eng. 36 , 1 - 9
    full text (url)
  • Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K. (2016):
    Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production
    Biotechnol. Bioeng. 113 (6), 1305 - 1314
    full text (url)
to index

2015 (5)

  • Lindmeyer, M., Jahn, M., Vorpahl, C., Müller, S., Schmid, A., Bühler, B. (2015):
    Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains
    Front. Microbiol. 6 , art. 1042
    full text (url)
  • Lindmeyer, M., Meyer, D., Kuhn, D., Bühler, B., Schmid, A. (2015):
    Making variability less variable: matching expression system and host for oxygenase-based biotransformations
    J. Ind. Microbiol. Biotechnol. 42 (6), 851 - 866
    full text (url)
  • Theodosiou, E., Frick, O., Bühler, B., Schmid, A. (2015):
    Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation
    Microb. Cell. Fact. 14 , art. 108
    full text (url)
  • Volmer, J., Schmid, A., Bühler, B. (2015):
    Guiding bioprocess design by microbial ecology
    Curr. Opin. Microbiol. 25 , 25 - 32
    full text (url)
  • Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K. (2015):
    Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation
    Biotechnol. Bioeng. 112 (9), 1738 - 1750
    full text (url)
to index

Under construction

Lecture: “Basics of Biocatalysis” in the 1st semester module “Introduction to Chemical Biotechnology”
Lecturer: Prof. Bruno Bühler

Start: October 13, 2017 (Fridays each weak, 3pm – 5pm or according to announcement)
Where: MLU Halle, Weinberg-Campus (Hoher Weg 8), SR 101 HW

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Lecture: “Whole-Cell Biocatalysis” in the 3rd semester module “Applied Biocatalysis”
Lecturer: Prof. Bruno Bühler

Start: October 13, 2017 (Fridays each weak, 10am – 12am or according to announcement)
Where: MLU Halle, Weinberg-Campus (Hoher Weg 8), SR 107 HW

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Project seminar / practical course on Applied Biocatalysis
Responsible supervisor: Prof. Bruno Bühler

Timing: February 5 – 9, 2018
Where: UFZ Leipzig

Under construction