.

Prof. Dr. Bruno Bühler

Tel.: +49-341-235 46 87
Fax: +49-341-235 45 12 86
Mail: bruno.buehler@ufz.de


Working Group:

Applied Biocatalysis


Research Interests

Photobiotechnology, (continuous) redox and multistep biocatalysis using microbial cell factories, highly productive and specific oxyfunctionalizations, metabolic engineering – heterologous pathway design and systems biotechnology in Cyanobacteria, Escherichia coli, Pseudomonads, and Corynebacterium glutamicum, reaction engineering, sustainability.


Education

01/2014

Habilitation, TU Dortmund University, Venia Legendi in Biotechnology and Technical Microbiology
 

1998 - 2003

PhD studies, Dr. sc. nat. ETH Swiss Federal Institute of Technology (ETH) Zurich,
Thesis: “Specific Oxyfunctionalization of Pseudocumene by Multistep Biocatalysis - Mechanism and Scale Up.
Supervision: Prof. Dr. A. Schmid, Prof. Dr. B. Witholt
Awarded with the ETH medal for outstanding PhD theses

1998

Diploma, Dipl. Natw. ETH Swiss Federal Institute of Technology (ETH) Zurich
Thesis: “Analysis of the mRNA Pattern of the pha Locus of Pseudomonas oleovorans and Evaluation of Possible Functions of the PhaF Protein”
Supervision: Dr. M. A. Prieto, Dr. B. Kessler, Prof. Dr. B. Witholt

1993 - 1998

Study of Biology/ Biochemistry, specialization in Biotechnology, Swiss Federal Institute
of Technology (ETH) Zurich, Switzerland


Positions

since 10/2015

Professor for Applied Biocatalysis
Faculty I of Natural Science – Biological Science, Martin Luther University Halle-Wittenberg, Halle, Germany

since 07/2015

Group Leader
Head of “Applied Biocatalysis Group” in the Department Solar Materials, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany

07/2015 - 12/2016

Visiting Scientist
Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, Dortmund, Germany

10/2014 - 06/2015

Visiting Scientist
Department Solar Materials, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany

11/2004 - 06/2015

Group Leader
Head of “Applied Biocatalysis Group” in the Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering,
TU Dortmund University, Dortmund, Germany

07/2003 - 10/2004

Post Doctoral Fellow
Institute of Biotechnology, ETH Zurich, Switzerland

06/1998 - 06/2003

Research Scientist
Institute of Biotechnology, ETH Zurich, Switzerland

Index:

2017 (6)
2016 (2)
2015 (5)

You could use our publication index for further requests.

2017 (6)

  • Hoschek, A., Bühler, B., Schmid, A., (2017):
    Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization
    Angew. Chem.-Int. Edit. 56 (47), 15146 - 15149
    full text (url)
  • Kadisch, M., Julsing, M.K., Schrewe, M., Jehmlich, N., Scheer, B., von Bergen, M., Schmid, A., Bühler, B., (2017):
    Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance
    Biotechnol. Bioeng. 114 (4), 874 - 884
    full text (url)
  • Kadisch, M., Schmid, A., Bühler, B., (2017):
    Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing
    J. Ind. Microbiol. Biotechnol. 44 (3), 339 - 351
    full text (url)
  • Kadisch, M., Willrodt, C., Hillen, M., Bühler, B., Schmid, A., (2017):
    Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts
    Biotechnology Journal 12 (8), art. 1600170
    full text (url)
  • Theodosiou, E., Breisch, M., Julsing, M.K., Falcioni, F., Bühler, B., Schmid, A., (2017):
    An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli
    Biotechnol. Bioeng. 114 (7), 1511 - 1520
    full text (url)
  • Volmer, J., Schmid, A., Bühler, B., (2017):
    The application of constitutively solvent-tolerant P. taiwanensis VLB120ΔCΔttgV for stereospecific epoxidation of toxic styrene alleviates carrier solvent use
    Biotechnology Journal 12 (7), art. 1600558
    full text (url)
to index

2016 (2)

  • Ladkau, N., Assmann, M., Schrewe, M., Julsing, M.K., Schmid, A., Bühler, B., (2016):
    Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli
    Metab. Eng. 36 , 1 - 9
    full text (url)
  • Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K., (2016):
    Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production
    Biotechnol. Bioeng. 113 (6), 1305 - 1314
    full text (url)
to index

2015 (5)

  • Lindmeyer, M., Jahn, M., Vorpahl, C., Müller, S., Schmid, A., Bühler, B., (2015):
    Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains
    Front. Microbiol. 6 , art. 1042
    full text (url)
  • Lindmeyer, M., Meyer, D., Kuhn, D., Bühler, B., Schmid, A., (2015):
    Making variability less variable: matching expression system and host for oxygenase-based biotransformations
    J. Ind. Microbiol. Biotechnol. 42 (6), 851 - 866
    full text (url)
  • Theodosiou, E., Frick, O., Bühler, B., Schmid, A., (2015):
    Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation
    Microb. Cell. Fact. 14 , art. 108
    full text (url)
  • Volmer, J., Schmid, A., Bühler, B., (2015):
    Guiding bioprocess design by microbial ecology
    Curr. Opin. Microbiol. 25 , 25 - 32
    full text (url)
  • Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K., (2015):
    Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation
    Biotechnol. Bioeng. 112 (9), 1738 - 1750
    full text (url)
to index

Index:

2015    (4)                  2007    (1)     
2014    (5)                  2006    (3)
2013    (7)                  2004    (1)
2012    (8)                  2003    (2)
2011    (5)                  2002    (2)
2010    (4)                  2000    (1)
2008    (4)                  1999    (1)


2015 (4)

  • Looße C., Galozzi S., Debor L., Julsing M. K., Bühler B., Schmid A., Barkovits K., Müller T. and Marcus K. (2015)
    Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples.
    EuPA Open Proteomics, 7:20-26, DOI-Link

  • Collins, J., Grund, M., Brandenbusch, C., Sadowski, G., Schmid, A., Bühler, B., (2015):The dynamic influence of cells on the formation of stable emulsions in organic–aqueous biotransformations
    J. Ind. Microbiol. Biotechnol. 42 (7), 1011 - 1026

  • Falcioni F., Bühler B. and Schmid A. (2015)
    Efficient hydroxyproline production from glucose in minimal media by Corynebacterium glutamicum.
    Biotechnology and Bioengineering, 112(2):322-330, DOI-Link

  • Brandenbusch C., Collins J., Glonke S., Hoffrogge R., Grunwald K., Bühler B., Schmid A. and Sadowski G. (2015)
    Process boundaries of irreversible scCO2-assisted phase separation in biphasic whole-cell biocatalysis.
    Biotechnology and Bioengineering, 112(11):2316-2323, DOI-Link

2014 (5)

  • Jahn M., Vorpahl C., Türkowsky D., Lindmeyer M., Bühler B., Harms H. and Müller S. (2014)
    Accurate determination of plasmid copy number on the single cell level using droplet digital PCR.
    Analytical Chemistry, 86(12):5969–5976, DOI-Link

  • Volmer J., Neumann C., Bühler B. and Schmid A. (2014)
    Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity.
    Applied and Environmental Microbiology, 80(20):6539-6548, DOI-Link

  • Willrodt C., David C., Cornelissen S., Bühler B., Julsing M. K. and Schmid A. (2014)
    Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.
    Biotechnology Journal, 9(8):1000–1012, DOI-Link

  • Schrewe M., Julsing M. K., Lange K., Czarnotta E, Schmid A. and Bühler B. (2014)
    Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.
    Biotechnology and Bioengineering, 111(9):1820-1830, DOI-Link

  • Ladkau N., Schmid A. and Bühler B. (2014)
    The microbial cell - functional unit for energy-dependent multistep biocatalysis.
    Current Opinion in Biotechnology, 30:178-189, DOI-Link

2013 (7)

  • Steen A., Ütkür F.Ö., Borrero-de Acuna J. M., Bunk B., Roselius L., Bühler B., Jahn D. and Schobert M. (2013)
    Construction and characterization of nitrate and nitrite respiring Pseudomonas putida KT2440 strains for anoxic biotechnical applications.
    Journal of Biotechnology, 163(2):155-165

  • Schrewe M., Ladkau N., Bühler B. and Schmid A. (2013)
    Direct terminal alkylamino-functionalization via multistep biocatalysis in one recombinant whole-cell catalyst.
    Advanced Synthesis and Catalysis, 355(9):1693-1697

  • Falcioni F., Blank L. M., Frick O., Karau A., Bühler B. and Schmid A. (2013)
    Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.
    Applied and Environmental Microbiology, 79(9):3091-3100

  • Jahn M., Seifert J., von Bergen M., Schmid A., Bühler B. and Müller S. (2013)
    Subpopulation-proteomics in prokaryotic populations.
    Current Opinion in Biotechnology, 24(1):79-87

  • Kuhn D., Fritzsch F. S. O., Zhang X., Wendisch V. F., Blank L. M., Bühler B. and Schmid A. (2013)
    Subtoxic product levels limit the epoxidation capacity of recombinant E. coli by increasing microbial energy demands.
    Journal of Biotechnology, 163(2):194-203, PubMed

  • Schrewe M.*, Julsing M. K.*, Bühler B. and Schmid A. (2013)
    Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.
    Chemical Society Reviews, 42(15):6346 - 6377
    * These authors contributed equally to this work.

  • Cornelissen S., Julsing M. K., Volmer J., Riechert O., Schmid A. and Bühler B. (2013)
    Whole-cell-based CYP153A6-catalyzed (S)-limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL.
    Biotechnology and Bioengineering, 110(5):1282-1292

2012 (8)

  • Cornelissen S., Julsing M. K., Schmid A. and Bühler B. (2012)
    Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis.
    Journal of Industrial Microbiology and Biotechnology, 39(2):275-287

  • Ütkür F.Ö., Tran T.-T., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
    Integrating organic-aqueous biocatalysis and product recovery using quinaldine 4-oxidase in living P. putida KT2440 (pKP1).
    Journal of Industrial Microbiology & Biotechnology, 39(7):1049-1059

  • Kuhn D., Bühler B. and Schmid A. (2012)
    Production host selection for asymmetric styrene epoxidation: Escherichia coli vs. solvent tolerant Pseudomonas.
    Journal of Industrial Microbiology and Biotechnology, 39(8):1125-1133

  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
    Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
    Biotechnology and Bioengineering, 109(5):1109-1119

  • Braun A., Geier M., Bühler B., Schmid A., Mauersberger S. and Glieder A. (2012)
    Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.
    Microbial Cell Factories, 11:106

  • Kuhn D., Julsing M. K., Heinzle E. and Bühler B. (2012)
    Systematic optimization of a biocatalytic two-liquid phase oxyfunctionalization process guided by ecological andeconomic assessment.
    Green Chemistry, 14(3):645 - 653

  • Julsing M. K.*, Schrewe M.*, Cornelissen S., Hermann I., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in E. coli.
    Applied and Environmental Microbiology, 78(16):5724-5733
    * These authors contributed equally to this work.

  • Escapa I. F., Garcia J. L., Bühler B., Blank L. M. and Prieto M.A. (2012)
    The polyhydroxyalkanoate metabolism controls carbon and energy spillage in Pseudomonas putida.
    Environmental Microbiology, 14(4):1049-1063

2011 (5)

  • Cornelissen S., Liu S, Deshmukh A.T., Schmid A. and Bühler B. (2011)
    Cell physiology can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.
    Journal of Industrial Microbiology and Biotechnology, 38:1359-1370

  • Ladkau N., Hermann I., Bühler B. and Schmid A. (2011)
    Enzyme catalyzed laurolactam synthesis via intramolecular amide bond formation in aqueous solution.
    Advanced Synthesis and Catalysis, 353(13):2501-2510

  • Hollmann F., Arends I. W. C. E, Bühler K., Schallmey A. and Bühler B. (2011)
    Enzyme-mediated oxidations for the chemist.
    Green Chemistry, 13:226-265

  • Schrewe M., Magnusson A., Willrodt C., Bühler B. and Schmid A. (2011)
    Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis.
    Advanced Synthesis and Catalysis, 353:3485 – 3495

  • Ütkür F.Ö., Gaykawad S., Bühler B. and Schmid A. (2011)
    Regioselective aromatic hydroxylation of quinaldine with water using quinaldine 4-oxidase in recombinant Pseudomonas putida.
    Journal of Industrial Microbiology and Biotechnology, 38(8):1067-1077

2010 (4)

  • Brandenbusch C., Bühler B., Hoffmann P., Sadowski G. and Schmid A. (2010)
    Efficient phase separation and product recovery in organic-aqueous bioprocessing using supercritical carbon dioxide.
    Biotechnology and Bioengineering, 107(4):642-651

  • Kuhn D., Kholiq A., Heinzle E., Bühler B. and Schmid A. (2010)
    Intensification and economic and ecological assessment of a biocatalytic oxyfunctionalization process.
    Green Chemistry, 12(5):815-827

  • Blank L. M., Ebert B. E., Bühler K. and Bühler B. (2010)
    Redox biocatalysis and metabolism: molecular mechanisms and metabolic network analysis.
    Antioxidants & Redox Signaling, 13(3):349-394, PubMed

  • Kuhn D., Blank L. M., Schmid A. and Bühler B. (2010)
    Systems biotechnology - rational biocatalyst and bioprocess design.
    Engineering in Life Sciences, 10(5):384–397

2008 (4)

  • Julsing M. K., Cornelissen S., Bühler B. and Schmid A. (2008)
    Heme-iron oxygenases: powerful industrial biocatalysts?
    Current Opinion in Chemical Biology, 12(2):177-186, PubMed

  • Blank L. M., Ebert B. E., Bühler B. and Schmid A. (2008)
    Metabolic capacity estimation of Escherichia coli as platform for redox biocatalysis: Constraint based modeling and experimental verification.
    Biotechnology and Bioengineering, 100(6):1050-1065, PubMed

  • Blank L. M., Ionidis G., Ebert B. E., Bühler B. and Schmid A. (2008)
    Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.
    FEBS Journal, 275(20):5173-5190, PubMed

  • Bühler B., Park J. B., Blank L. M. and Schmid A. (2008)
    NADH availability limits asymmetric biocatalytic epoxidation in growing recombinant Escherichia coli.
    Applied and Environmental Microbiology, 74(5):1436-1446, PubMed

2007 (1)

  • Park J. B., Bühler B., Panke S., Witholt B. and Schmid A. (2007)
    Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent tolerant Pseudomonas sp. strain VLB120ΔC.
    Biotechnology and Bioengineering, 98(6):1219-1229, PubMed

2006 (3)

  • Bühler B., Straathof A. J. J., Witholt B. and Schmid A. (2006)
    Analysis of two-liquid-phase multistep biooxidation based on a process model: Indications for biological energy shortage.
    Organic Process Research and Development, 10(3):628-643

  • Meyer D., Bühler B. and Schmid A. (2006)
    Process and catalyst design objectives for specific redox biocatalysis.
    Advances in Applied Microbiology, 59:53-91, PubMed

  • Park J. B.*, Bühler B.*, Habicher T., Hauer B., Panke S., Witholt B. and Schmid A. (2006)
    The efficiency of recombinant Escherichia coli as biocatalyst for stereospecific epoxidation.
    Biotechnology and Bioengineering, 95(3):501-512, PubMed
    * These authors contributed equally to this work.

2004 (1)

  • Bühler B. and Schmid A. (2004)
    Process implementation aspects for biocatalytic hydrocarbon oxyfunctionalisation.
    Journal of Biotechnology, 113(1-3):183-210, PubMed

2003 (2)

  • Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
    Chemical biotechnology for the specific oxyfunctionalization of hydrocarbons an a technical scale.
    Biotechnology and Bioengineering, 82(7):833-842, PubMed

  • Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
    Use of the two-liquid phase concept to exploit kinetically controlled multistep biocatalysis.
    Biotechnology and Bioengineering, 81(6):683-694, PubMed

2002 (2)

  • Bühler B., Witholt B., Hauer B. and Schmid A. (2002)
    Characterization and application of xylene monooxygenase for multistep biocatalysis.
    Applied and Environmental Microbiology, 68(2):560-568, PubMed

  • Schmid A., Hollmann F., Park J. B. and Bühler B. (2002)
    The use of enzymes in the chemical industry in Europe.
    Current Opinion in Biotechnology, 13(4):359-366, PubMed

2000 (1)

  • Bühler B., Schmid A., Hauer B. and Witholt B. (2000)
    Xylene monooxygenase catalizes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101.
    Journal of Biological Chemistry, 275(14):10085-100092, PubMed

1999 (1)

  • Prieto M.A., Bühler B., Jung K., Witholt B. and Kessler B. (1999)
    PhaF, a polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1 involved in the regulatory expression system of pha genes.
    Journal of Bacteriology, 181(3):858-868, PubMed

2017

  • Schmid A., Hoschek A., and Bühler B.
    Verfahren zur bioreaktiven Extraktion erzeugten Sauerstoffs aus einem Reaktionsraum, sowie Verwendung von phototrophen Mikroorganismen bei der Gewinnung von Wasserstoff
    Germany, DE 10 2017 104 648 A1, filed
    International, PCT/EP2018/055449, filed


2013

  • Sadowski G., Brandenbusch C., Collins J. and Bühler B. (2013)
    Applied catastrophic phase inversion.
    Europe, EP 13 005 234.3, priority: 6.11.2013, filed

2011

  • Schmid A., Bühler B., Ladkau N. and Hermann I. (2011)
    Method for omega-aminocarboxylic acid condensation.
    Europe, WO/2012/156151 / PCT/EP2012/056251 /priority: 11004029.2 16.05.2011 EP

2010

  • Pötter M., Schmid A., Bühler B., Hennemann H.-G., Julsing M. K., Schaffer S., Haas T., Schrewe M., Cornelissen S., Roos M. and Häger H. (2010)
    Biocatalytic oxidation process with alkL-gene product.
    WO 2011/131420 / PCT/EP2011/053834 /priority: 10 2010 015 807.0 20.04.2010 DE

2007

  • Sadowski G., Schmid A., Brandenbusch C., Bühler B. and Görnert M. (2007)
    Method for processing coalescence-inhibited emulsions from whole-cell biotransformations with compressed or supercritical gases.
    WO/2009/012754 / EP2170481 / DE 10 2007 034 258.8 / US 8,431,358 B2

  • Karau A., Sieber V., Haas T., Häger H., Grammann K., Bühler B., Blank L. M. and Schmid A. (2007)
    Omega-amino carboxylic acids, omega-amino carboxylic acid esters, or recombinant cells which produce lactams thereof.
    WO/2009/077461 / PCT/EP2008/067447 / priority: 102007060705.0 17.12.2007 DE


2001

  • Schmid A., Hauer B., Bühler B. and Witholt B. (2001)
    Method for the oxidation of aromatic compunds.
    WO/2002/081718 / PCT/EP2002/003803 / priority: 101 17 359.8 06.04.2001 DE


1999

  • Schmid A., Bühler B., Hauer B. and Witholt B. (1999)
    Microbiological method for producing aromatic aldehydes and/or carboxylic acids.
    WO/2001/031047 / PCT/EP2000/010552 / priority: 199 51 768.1 27.10.1999 DE

2012

  • Hollmann F., Bühler K. and Bühler B. (2012)
    Oxidation of Alcohols, Aldehydes, and Acids.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1325-1437, ISBN: 978-3-527-32547-4

  • Bühler B., Bühler K. and Hollmann F. (2012)
    Oxyfunctionalization of C-C-Multiple Bonds.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1269-1324, ISBN: 978-3-527-32547-4

  • Ütkür F.Ö., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
    Regioselective Aromatic Hydroxylation of Quinaldine Using Living Pseudomonas putida Cells Containing Quinaldine 4-oxidase.
    in John Whitthal and Peter Sutton Practical Methods in Biocatalysis and Biotransformations 2.
    John Wiley & Sons Ltd, Chichester, UK:153-157, ISBN: 978-1-119-99139-7


2011

  • Brandenbusch C., Bühler B., Sadowski G. and Schmid A. (2011)
    Biokatalytische Epoxidierung von Styrol zu enantiomerenreinem (S)-Styroloxid.
    in Chmiel H. Bioprozesstechnik.
    Spektrum Akademischer Verlag, Heidelberg, Germany:486-488, ISBN: 978-3-8274-2476-1

  • Ütkür F.Ö. and Bühler B. (2011)
    Molybdenum-Containing Enzymes and their Applications.
    in Ortiz M. and Herrera T. (eds.) Molybdenum: Characteristics, Production and Applications.
    Nova Science Publishers, Inc., Hauppauge, NY, USA:117-146, ISBN: 978-1-61470-504-8


2009

  • Bühler B., Blank L. M., Ebert B. E., Bühler K. and Schmid A. (2009)
    Energy and cofactor issues in fermentation and oxyfunctionalisation processes.
    in Smolke C. D. The metabolic pathway engineering handbook.
    CRC Press, Boca Raton, Florida, USA, Vol. 2:21 1-32, ISBN: 978-1-4200-7765-0


2008

  • Eissen M., Geisler G., Bühler B., Fischer C., Hungerbuehler K., Schmid A. and Carreira E. M. (2008)
    Mass Balances and Life Cycle Assessment.
    in Lapkin A. and Constable D. Green Chemistry Metrics: Measuring and Monitoring Sustainable Processes.
    John Wiley & Sons Ltd, Chichester, West Sussex, UK:200-227


2005

  • Bühler B. and Schmid A. (2005)
    Selective enzymatic hydroxylations.
    in Dyker G. Handbook of C-H transformations.
    Wiley-VCH, Weinheim, Germany, Vol. 2:516-529


2003

  • Bühler B. (2003)
    PhD Thesis: Specific oxyfunctionalization of pseudocumene by multistep biocatalysis - mechanism and scale up.
    Swiss Federal Institute of Technology (ETH), Zurich, Switzerland


2002

  • Hollmann F., Bühler B. and Schmid A. (2002)
    Oxidation of alcohols, phenols, aldehydes, acids and C-N bonds.
    in Drauz K. and Waldmann H. Enzyme catalysis in organic synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. III:1108-1202, 1245-1261,ISBN: 3-527-29949-1

2017

  • Bühler B. (2017)
    Status and prospects of microbial biocatalysis.
    Bioprocessing India 2017, December 9th – 11th 2017, Guwahati, India.


2016

  • Bühler B. (2016)
    Productive two-liquid phase whole-cell biocatalysis on the verge of industrial implementation.
    Seminar at the Department of Biotechnology at the TU Delft, 7th of January 2016, Delft, The Netherlands

  • Bühler B. (2016)
    Integrated bioprocess development for whole-cell biocatalysis .
    Fields of Expertise meeting, TU Graz, 3rd of November 2016, Graz, Austria

  • Bühler B. (2016)
    Productive whole-cell oxygenase and cascade biocatalysis.
    2016 KSBB Spring Meeting and International Symposium, 21st - 22nd of April 2016, Gyeongju, South Korea

  • Bühler B. and Maskow T. (2016)
    Biotechnology for the chemical industry.
    UFZ EnergyDays 2016, 22nd - 23rd of March 2016, Leipzig, Germany

  • Bühler B. (2016)
    Whole-cell biocatalysis - status and future prospects.
    Industrial Biocatalysis; 8th Wädenswil Day of Life Science hosts CCBIO Symposium, 20th of June 2016, Wädenswil, Switzerland

  • Bühler B., Schrewe M., Ladkau N., Falcioni F., Julsing M. K. and Schmid A. (2016)
    The microbial cell – functional unit for multistep biocatalysis.
    Seminar at Ewha Womans University, 19th of April 2016, Seoul, South Korea

  • Bühler B. (2016)
    Productive whole-cell biocatalysis.
    Conference on Green Chemistry and White Biotechnology, 12th - 13th of May 2016, Gembloux, Belgium

  • Bühler B., Volmer J., Lindmeyer M. and Schmid A. (2016)
    How to profit from the promising properties of Pseudomonads for productive whole-cell biocatalysis.
    European Symposium on Biochemical Engineering Sciences (ESBES) 2016, 11th -14th of September 2016, Dublin, Ireland


2015

  • Bühler B., Volmer J., Lindmeyer M. and Schmid A. (2015)
    Exploiting and engineering solvent tolerance of Pseudomonads for whole-cell biocatalysis.
    Sino-¬German Symposium on Biobased Chemicals and Biorefinery, 8. - 9. October 2015, Frankfurt, Germany

  • Bühler B. (2015)
    Productive microbial redox and multistep biocatalysis utilizing renewable resources.
    Workshop: Biotechnologie der stofflichen und energetischen Nutzung erneuerbarer Ressourcen, 1st - 2nd of December, UFZ Leipzig, Germany

2014

  • Bühler B. (2014)
    Biocatalyst and reaction engineering for productive whole-cell biocatalysis.
    Sino-German Symposium on Biobased Chemicals and Biorefinery, 7. - 9. October 2014, Beijing, China

  • Bühler B., Schrewe M., Ladkau N., Falcioni F., Julsing M. K. and Schmid A. (2014)
    The microbial cell - functional unit for multistep biocatalysis.
    3rd Multistep Enzyme Catalyzed Processes Congress (MECP14), 7. - 10. April 2014, Madrid, Spain

2013

  • Bühler B., Schrewe M., Julsing M. K., Ladkau N., Cornelissen S., Hillen M, Falcioni F., Kuhn D., Volmer J., Lindmeyer M., Blank L. M. and Schmid A. (2013)
    Orthologous reaction and pathway engineering enabling productive whole-cell biocatalysis.
    Seminar of the Institute of Applied Synthetic Chemistry, TU Vienna, 24. October 2013, Vienna, Austria

  • Bühler B. (2013)
    Biotechnologie für die Chemieindustrie - Biocatalyst and Reaction Engineering for Productive Multistep and Redox Biocatalysis .
    Tag des BCI, TU Dortmund, 11. October 2013, Dortmund, Germany

  • Bühler B., Schrewe M., Julsing M. K., Ladkau N., Cornelissen S., Hillen M, Falcioni F., Kuhn D., Volmer J., Blank L. M. and Schmid A. (2013)
    Biocatalyst and Reaction Engineering for Productive Multistep and Redox Biocatalysis.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK

  • Bühler B., Falcioni F., Ladkau N., Theodosiou E., Schrewe M., Fu J., Bühler K., Julsing M. K. and Schmid A. (2013)
    Engineering of microbes for oxyfunctionalization and multistep biocatalysis based on renewable resources.
    Bio-Alternatives 2013, 3. - 4. April 2013, Graz, Austria

2012

  • Bühler B., Ladkau N., Schrewe M., Julsing M. K. and Schmid A. (2012)
    Terminal oxy- and aminofunctionalization of fatty acid methyl esters via heterologous pathway engineering.
    ESBES - 9th European Symposium on Biochemical Engineering Science, 23. - 26. September 2012, Istanbul, Turkey

  • Bühler B., Kuhn D., Schrewe M., Julsing M. K., Ladkau N., Ütkür F.Ö., Cornelissen S., Blank L. M. and Schmid A. (2012)
    Metabolic and bioprocess engineering for productive multistep and redox biocatalysis.
    2nd Biocatalysis Symposium, EMPA, 16. January 2012, EMPA, Dübendorf, Switzerland

2011

  • Bühler B., Collins J., Brandenbusch C., Kuhn D., Park J. B., Schrewe M., Blank L. M., Sadowski G. and Schmid A. (2011)
    Whole-cell biocatalysis in organic-aqueous two-liquid phase systems.
    Frontiers 2011, 21. - 22. June 2011, Delft, The Netherlands

  • Bühler B. (2011)
    Rational strain and process engineering for efficient whole-cell redox biocatalysis.
    DBU-Workshop „Effiziente Biokatalysatoren für die Weiße Biotechnologie“, 2. - 3. May 2011, Osnabrück, Germany

2010

  • Bühler B., Kuhn D., Ebert B. E., Blank L. M. and Schmid A. (2010)
    Ecoefficiency and metabolic evaluation of recombinant microbial biocatalysts for specific carbon oxyfunctionalization.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany

  • Bühler B., Blank L. M. and Schmid A. (2010)
    Biocatalysis meets Systems Biotechnology.
    Jahrestagung der Biotechnologen, 21. - 23. September, Aachen, Germany

  • Bühler B., Falcioni F., Frick O., Fu J., Blank L. M., Bühler K. and Schmid A. (2010)
    Coupling of central carbon metabolism to selective proline hydroxylation by α-ketoglutarate-dependent proline 4-hydroxylase.
    ESBES (European Symposium on Biochemical Engineering Science), 5. - 8. March 2010, Bologna, Italy

2009

  • Bühler B., Bühler K., Blank L. M. and Schmid A. (2009)
    The use of oxidoreductases for biocatalytic oxyfunctionalization.
    NAWI Graz Workshop, 26th of June 2009, Graz, Austria

  • Bühler B. (2009)
    The use of oxidoreductases for biocatalytic oxyfunctionalization.
    GDCh-Vortrag, Greifswald, Germany

2008

  • Bühler B., Kuhn D., Ebert B. E., Kholiq A., Heinzle E., Blank L. M. and Schmid A. (2008)
    Using Systems Biotechnology and Eco-Efficiency Analyses to Enable Sustainable Redox Biocatalysis.
    Bioperspectives 2008, 7th - 9th of October, Hannover, Germany

  • Bühler B., Ionidis G., Park J. B., Ebert B. E., Kuhn D., Blank L. M. and Schmid A. (2008)
    Systems Biotechnology for Selective Whole-Cell Biocatalysis.
    ESBES 2008, 7th - 10th of September 2008, Faro, Portugal

  • Bühler B. (2008)
    Whole-Cell Biocatalysis for Carbon Oxyfunctionalization.
    Seminar at the Medical Proteome Center Bochum, 9th of May 2008, Bochum, Germany

2007

  • Bühler B., Ebert B. E., Blank L. M. and Schmid A. (2007)
    Systems biotechnology of resting Escherichia coli cells as a platform for sustainable redox biocatalysis.
    European BioPerspectives, 30. May - 1. June 2007, Cologne, Germany

2006

  • Bühler B. and Schmid A. (2006)
    Whole-cell biocatalysis for the oxyfunctionalization of hydrocarbons - efficiency and limitations.
    24. DECHEMA-Jahrestagung der Biotechnologen, 26. - 28. September 2006, Wiesbaden, Germany

  • Bühler B. and Schmid A. (2006)
    Whole-cell biocatalysis for the oxyfunctionalization of hydrocarbons-efficiency and limitations.
    Institute of Biotechnology, Forschungszentrum Jülich, Germany

2005

  • Bühler B., Straathof A. J. J. and Schmid A. (2005)
    Whole-cell bio-oxidation in a two-liquid phase system. What are limiting factors?
    Biocatalysis in Nonconventional Media, 13. - 14. April 2005, Manchester, UK

  • Bühler B., Park J. B., Witholt B. and Schmid A. (2005)
    Application of oxygenases in whole-cell biocatalysis; two examples.
    Public Lecture at the Department of Biology, University of Konstanz, Germany

2004

  • Bühler B., Straathof A. J. J. and Schmid A. (2004)
    Modeling and simulation of a whole-cell multistep biooxidation process in a two-liquid phase system.
    5th European Symposium on Biochemical Engineering Science, ESBES-5, 8. - 11. September 2004, Stuttgart, Germany


2001

  • Bühler B., Witholt B., Hauer B. and Schmid A. (2001)
    Multistep biocatalysis with Xylene Monooxygenase containing recombinant E. coli; Production of 3,4-dimethylbenzaldehyde from pseudocumene.
    VAAM-Biotransformation Jahrestagung, Berlin, Germany