.

Prof. Dr. Katja Bühler

Tel.: +49-341-235 46 83
Fax: +49-341-235 45 12 86
Mail: katja.buehler@ufz.de


Working Group:

Catalytic Biofilms


Teaching:

Professur TU Dresden


Research Interests

Development of alternative biotechnological reactor concepts based on in vitro and in vivo approaches.


Education

11/2013   Venia Legendi for microbiology
Title 'Biotechnology & Catalytic Biofilms'
1998 - 2001   PhD (Dr. rer. nat.) at the Technical University Hamburg-Harburg, Institut of Biotechnology II with Prof. Rudolf Müller
Title: 'Characterization of a thermostable alkane oxidizing system'
1993 - 1998   Academic studies of Biology at the University Hamburg
Focus on Microbiology und Biochemistry. Degree as Dipl. Biol.


Professional career

since 12/2015   Professor for 'Technology of productive Biofilms' at the Technical University Dresden in a joint professorship with the Helmholtzcenter for Environmental Research UFZ, Leipzig, Germany
2004 - 2015   Groupleader ‘Technical Enzymology’ Chair of Chemical Biotechnology (Prof. Andreas Schmid),
Dept. Biochemical and Chemical Engineering, TU Dortmund, Germany
2001 - 2004   Postdoctoral Researcher Institut of Biotechnology, Prof. Bernard Witholt, ETH Zurich, Switzerland
1999 - 2001   Visiting Scientist Institut of Biotechnology, Prof. Bernard Witholt, ETH Zurich, Switzerland
1998 - 2001   Research Scientist Institut of Biotechnology II, TU Hamburg-Harburg, Germany

Index:

2018 (1)
2017 (5)
2016 (3)
2015 (4)

You could use our publication index for further requests.

2018 (1)

  • David, C., Schmid, A., Adrian, L., Wilde, A., Bühler, K., (2018):
    Production of 1,2-propanediol in photoautotrophic Synechocystis is linked to glycogen turn-over
    Biotechnol. Bioeng. 115 (2), 300 - 311
    full text (url)
    Data DOI
to index

2017 (5)

  • Buehler, K., (ed., 2017):
    Advances in catalytic biofilms
    Bioengineering (Special issue),
    full text (url)
  • Halan, B., Vassilev, I., Lang, K., Schmid, A., Buehler, K., (2017):
    Growth of Pseudomonas taiwanensis VLB120ΔC biofilms in the presence of n-butanol
    Microb. Biotechnol. 10 (4), 745 - 755
    full text (url)
  • Karande, R., Salamanca, D., Schmid, A., Buehler, K., (2017):
    Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120
    Biotechnol. Bioeng. 115 (2), 312 - 320
    full text (url)
    Data DOI
  • Schmutzler, K., Kupitz, K., Schmid, A., Buehler, K., (2017):
    Hyperadherence of Pseudomonas taiwanensis VLB120ΔC increases productivity of (S)-styrene oxide formation
    Microb. Biotechnol. 10 (4), 735 - 744
    full text (url)
  • Willrodt, C., Halan, B., Karthaus, L., Rehdorf, J., Julsing, M.K., Buehler, K., Schmid, A., (2017):
    Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow
    Biotechnol. Bioeng. 114 (2), 281 - 290
    full text (url)
to index

2016 (3)

  • Halan, B., Karande, R., Buehler, K., Schmid, A., (2016):
    Catalytic Pseudomonas taiwanensis VLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor
    J. Flow Chem. 6 (1), 39 - 42
    full text (url)
  • Karande, R., Schmid, A., Buehler, K., (2016):
    Applications of multiphasic microreactors for biocatalytic reactions
    Org. Process Res. Dev. 20 (2), 361 - 370
    full text (url)
  • Schmutzler, K., Kracht, O.N., Schmid, A., Buehler, K., (2016):
    Trophic regulation of autoaggregation in Pseudomonas taiwanensis VLB120
    Appl. Microbiol. Biotechnol. 100 (1), 347 - 360
    full text (url)
to index

2015 (4)

  • David, C., Bühler, K., Schmid, A., (2015):
    Stabilization of single species Synechocystis biofilms by cultivation under segmented flow
    J. Ind. Microbiol. Biotechnol. 42 (7), 1083 - 1089
    full text (url)
  • Karande, R., Debor, L., Salamanca, D., Bogdahn, F., Engesser, K.-H., Buehler, K., Schmid, A., (2015):
    Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms
    Biotechnol. Bioeng. 113 (1), 52 - 61
    full text (url)
  • Lang, K., Buehler, K., Schmid, A., (2015):
    Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms
    Adv. Synth. Catal. 357 (8), 1919 - 1927
    full text (url)
  • Schmutzler, K., Schmid, A., Buehler, K., (2015):
    A three-step method for analysing bacterial biofilm formation under continuous medium flow
    Appl. Microbiol. Biotechnol. 99 (14), 6035 - 6047
    full text (url)
to index

Index

2014    (7)                2008    (2)
2013    (1)                2007    (2)
2012    (1)                2006    (1)
2011    (4)                2005    (1)
2010    (5)                2004    (1)
2009    (2)


2014 (7)

  • Tomaszewski B., Schmid A. and Bühler K. (2014)
    Biocatalytic production of catechols using a high pressure tube-in-tube segmented flow microreactor.
    Organic Process Research & Development, 18(11):1516–1526


  • Ruinatscha R., Bühler K. and Schmid A. (2014)
    Development of a high performance electrochemical cofactor regeneration modul and its application for the continuos reduction of FAD.
    Journal of Molecular Catalysis B, 103:100–105

  • Churakova E.*, Tomaszewski B.*, Bühler K., Schmid A., Arends I. W. C. E and Hollmann F. (2014)
    Hydrophobic formic acid esters for cofactor regeneration in aqueous / organic two-liquid phase systems.
    Topics in Catalysis, 57(5):385-391
    * These authors contributed equally to this work.

  • Lang K., Zierow J., Bühler K. and Schmid A. (2014)
    Metabolic engineering of Pseudomonas sp. VLB120 towards a platform biocatalyst for the production of isobutyric acid and other secondary metabolites.
    Microbial Cell Factories, 13:2


  • Tomaszewski B., Lloyd R., Warr A., Bühler K. and Schmid A. (2014)
    Regioselective biocatalytic aromatic hydroxylation in a gas-liquid multiphase tube-in-tube reactor.
    ChemCatChem, 6(9):2567–2576

  • Halan B., Letzel T., Schmid A. and Bühler K. (2014)
    Scalability of a solid support membrane aerated biofilm reactor for the continuous synthesis of (S)-styrene oxide.
    Biotechnology Journal, 9(10):1339-1349

  • Karande R.*, Halan B.*, Schmid A. and Bühler K. (2014)
    Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.
    Biotechnology and Bioengineering, 111(9):1831-1840
    * These authors contributed equally to this work.

2013(1)

  • Gross R., Bühler K. and Schmid A. (2013)
    Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide.
    Biotechnology and Bioengineering, 110(2):424–436

2012 (1)

  • Halan B., Bühler K. and Schmid A. (2012)
    Biofilms as living catalysts in chemical synthesis.
    Trends in Biotechnology, 30(9):453-465


2011(4)

  • Hollmann F., Arends I. W. C. E, Bühler K., Schallmey A. and Bühler B. (2011)
    Enzyme-mediated oxidations for the chemist.
    Green Chemistry, 13:226-265

  • Ruinatscha R., Karande R., Bühler K. and Schmid A. (2011)
    Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using Sepabead EC-EA and EC-Q1A anion-exchange carriers.
    Molecules, 16(7):5975-5988

  • Karande R., Schmid A. and Bühler K. (2011)
    Miniaturizing Biocatalysis: Enzyme catalyzed reactions in an aqueous/organic segmented flow capillary microreactor.
    Advanced Synthesis and Catalysis, 353(13):2511-2521

  • Halan B., Schmid A. and Bühler K. (2011)
    Real time solvent tolerance analysis of Pseudomonas sp. strain VLB120ΔC catalytic biofilms.
    Applied and Enviromental Microbiology, 77(5):1563-1571


2010 (5)

  • Hollmann F., Arends I. W. C. E and Bühler K. (2010)
    Biocatalytic redox reactions for Organic Synthesis: Nonconventional regeneration methods.
    ChemCatChem, 2(7):762-782

  • Gross R., Lang K., Bühler K. and Schmid A. (2010)
    Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis.
    Biotechnology and Bioengineering, 105(4):705-717

  • Karande R., Schmid A. and Bühler K. (2010)
    Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity.
    Langmuir, 26(11):9152-9159

  • Halan B., Schmid A. and Bühler K. (2010)
    Maximizing the productivity of catalytic biofilms on solid supports in membrane-aerated reactors.
    Biotechnology and Bioengineering, 106(4):516-527

  • Blank L. M., Ebert B. E., Bühler K. and Bühler B. (2010)
    Redox biocatalysis and metabolism: molecular mechanisms and metabolic network analysis.
    Antioxidants & Redox Signaling, 13(3):349-394


2009 (2)

  • Rosche B., Li X. Z., Schmid A. and Bühler K. (2009)
    Microbial biofilms: A concept for industrial catalysis?
    TRENDS in Biotechnology, 27(11):636-643

  • Ruinatscha R., Dusny C., Bühler K. and Schmid A. (2009)
    Productive asymmetric styrene epoxidation based on a next generation electroenzymatic methodology.
    Advanced Synthesis and Catalysis, 351:2505-2515


2008 (2)

  • Tee K. L., Dmytrenko O., Otto K., Schmid A. and Schwaneberg U. (2008)
    A p-nitrothiophenolate screening system for the directed evolution of a two-component epoxygenase (StyAB).
    Journal of Molecular Catalysis B: Enzymatic, 50(2-4):121-127


  • Höllrigl V., Hollmann F., Kleeb A., Bühler K. and Schmid A. (2008)
    TADH, the thermostable alcohol dehydrogenase from Thermus sp. ATN1: A versatile new biocatalyst for organic synthesis.
    Applied Microbiology and Biotechnology, 81(2):263-273


2007 (2)

  • Höllrigl V., Otto K. and Schmid A. (2007)
    Electroenzymatic asymmetric reduction of rac-3-methylcyclohexanone to (1S,3S)-3-methylcyclohexanol in organic/aqueous media catalyzed by a thermophilic alcohol dehydrogenase.
    Advanced Synthesis & Catalysis, 349(8-9):1337-1340, DOI-Link

  • Gross R., Hauer B., Otto K. and Schmid A. (2007)
    Microbial biofilms: New catalysts for maximising productivty of long-term biotransformations.
    Biotechnology and Bioengineering, 98(6):1123-1134


2006 (1)

  • Ruinatscha R.*, Höllrigl V.*, Otto K. and Schmid A. (2006)
    Productivity of selective electroenzymatic reduction and oxidation reactions: Theoretical and practical considerations.
    Advanced Synthesis & Catalysis, 348(15):2015-2026
    * These authors contributed equally to this work.


2005 (1)

  • Hollmann F., Kleeb A., Otto K. and Schmid A. (2005)
    Coupled chemoenzymatic transfer hydrogenation catalysis for enantioselective reduction and oxidation reactions (Corrigendum in Tetrahedron-Asymmetry (2006), 17:867-868).
    Tetrahedron-Asymmetry, 16(21):3512-3519


2004 (4)

  • Otto K., Hofstetter K., Röthlisberger M., Witholt B. and Schmid A. (2004)
    Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component non-heme flavin-diffusible styrene monooxigenase.
    Journal of Bacteriology, 186(16):5292-5302

Patent Applications

2018

  • Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A.
    Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
    WO/2018/046104

2011

  • Schmid A., Karande R. and Bühler K. (2011)
    Segmented Flow Biofilm Reactor.
    WO/2012/152337


Books and Bookchapter

2017

  • Karande R., Schmid A. and Bühler K. (2017)
    Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
    in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
    Springer, Berlin, Germany

2012

  • Bühler K. and Schmid A. (2012)
    Catalytic biofilms: a powerful concept for future bioprocesses.
    in Lear, G. and Lewis, G. Microbial biofilms: Current research and applications.
    Caister Academic Press, Norfolk, UK:193 - 222, ISBN: 978-1-904455-96-7

  • Hollmann F., Bühler K. and Bühler B. (2012)
    Oxidation of Alcohols, Aldehydes, and Acids.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1325-1437, ISBN: 978-3-527-32547-4

  • Bühler B., Bühler K. and Hollmann F. (2012)
    Oxyfunctionalization of C-C-Multiple Bonds.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1269-1324, ISBN: 978-3-527-32547-4


2009

  • Bühler K. and Schmid A. (2009)
    Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
    in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
    Springer, Berlin, Germany, Vol. 1, ISBN: 3540775889

  • Bühler B., Blank L. M., Ebert B. E., Bühler K. and Schmid A. (2009)
    Energy and cofactor issues in fermentation and oxyfunctionalisation processes.
    in Smolke C. D. The metabolic pathway engineering handbook.
    CRC Press, Boca Raton, Florida, USA, Vol. 2:21 1-32, ISBN: 978-1-4200-7765-0

  • Bühler K. and Schmid A. (2009)
    Synthesis of enantiopure (S)-styrene oxide by selective oxidation of styrene by recombinant E. coli.
    in John Witthall and Peter Sutton Practical Methods in Biocatalysis and Biotransformations.
    John Wiley & Sons Ltd, Chichester, UK:385-390, ISBN: 978-0-470-51927-1


2001

  • Otto K. (2001)
    PhD Thesis: Characterization of a thermostable alkane oxidizing system.
    Technical University Hamburg-Harburg, Hamburg, Germany