Dr. Ivonne Nijenhuis
Contact / Address:
Phone + 49 (0) 341 6025 1356
Helmholtz Centre for Environmental Research - UFZ
Permoserstrasse 15
04318 Leipzig
Curriculum Vitae
2004 -current |
Senior Scientist, Department Technical Biogeochemistry (up to 2023 Isotope Biogeochemistry) |
from 04.2022 | Member of the Works Council |
09.2022-12.2023 | Staff Unit Change Manangement // Internationalisation Consultant |
04.2020- 08.2022 | Scientific Manager Integration Platform 'Tapping nature's potential for sustainable production and a healthy environment-TapNature'; Research Unit Environmental Engineering and Biotechnology |
2009-2020 | Working Group Leader 'organohalide geomicrobiology' |
2016 | Habilitation in natural sciences, Martin-Luther-University Halle-Wittenberg, Germany |
2013 | Certified Manager, Helmholtz Academy for managers Malik Management Centre St. Gallen |
2010-2015 | Coordinator European Union FP7 'People' programme Initial Training Network CSI:ENVIRONMENT |
2009-2017 | Elected employee representative member of the supervisory board of the UFZ |
2002 - 2004 |
Post-Doctoral Scientist EU Marie Curie Host Development fellowship (BIOISOTOPE) European expert in Anaerobic Microbiology, UFZ-Leipzig |
2002 | Ph.D. in Microbiology; Cornell University, USA |
1996 | M.Sc. in Environmental Sciences; Wageningen Agricultural University, the Netherlands |
Professional & Research interests
My research in the Department of technical Biogeochemistry focuses on the anaerobic biotransformation of halogenated organic contaminants and the application of stable isotope concepts. Currently, these are applied in the BMBF funded project KONATES, addressing the question on how aquifer thermal energy storage affects the in situ biotransformation of halogenated groundwater contaminants, i.e. chlorinated ethenes.
My main expertise include 1) understanding of microbial transformation of chlorinated and brominated ethenes and ethanes, halogenated pesticides, e.g. hexachlorocyclohexane, and pharmaceuticals, e.g. diclofenac, and 2) development and application of multi-element compound-specific stable isotope (C, Cl, Br, H) fractionation as a tool to analyse microbial reactions and biotransformation in situ.
Projects
2011-2019 the DFG Forschergruppe "FOR1530 'Anaerobic Dehalogenation: Organisms, Biochemistry and (Eco-) Physiology'" links research groups in Jena, Halle and Leipzig. Within this project, I was in charge of sub-project 05: 'Characterisation of microbial dehalogenation using compound specific stable isotope analysis'.
Within the European Union FP7 project "GENESIS" we investigated how biogeochemical processes affect natural attenuation of chlorinated benzenes and are focusing on the microbial community involved in contaminant biotransformation.
From 2010-2015, I coordinated the European Union FP7 'People' programme Initial Training Network "CSI:ENVIRONMENT". In this project, 10 Partners and 4 Associated Partners from 9 European countries will work on 'Isotope forensics meets biogeochemistry - linking sources and sinks of organic contaminants by compound specific isotope investigation'.
2012-2014, Joint German-Israeli Water Technology Programme “Development of innovative tools for the assessment of in situ biodegradation of organobromine contaminant in groundwater” – INTIME. In this 3 year project we will investigate the application of CSIA for assessment of the natural attenuation of brominated groundwater contaminants.
Publications (in co-operation with the UFZ)
Enantioselective carbon stable isotope fractionation of hexachlorocyclohexane during aerobic biodegradation by Sphingobium spp.
Environ. Sci. Technol. 47 (20), 11432 - 11439 10.1021/es402197s
Anaerobic biotransformation of hexachlorocyclohexane isomers by Dehalococcoides species and an enrichment culture
Biodegradation 29 (4), 409 - 418 10.1007/s10532-018-9838-9
Effect of temperature on microbial reductive dehalogenation of chlorinated ethenes: a review
FEMS Microbiol. Ecol. 98 (9), fiac081 10.1093/femsec/fiac081
Carbon stable isotope fractionation of sulfamethoxazole during biodegradation by Microbacterium sp. strain BR1 and upon direct photolysis
Environ. Sci. Technol. 49 (10), 6029 - 6036 10.1021/acs.est.5b00367
Evaluation of the hydrological flow paths in a gravel bed filter modeling a horizontal subsurface flow wetland by using a multi-tracer experiment
Sci. Total Environ. 621 , 265 - 272 10.1016/j.scitotenv.2017.11.217
Variability in microbial carbon isotope fractionation of tetra- and trichloroethene upon reductive dechlorination
Chemosphere 71 (4), 639 - 648 10.1016/j.chemosphere.2007.11.013
Tetrachloroethene conversion to ethene by a Dehalococcoides-containing enrichment culture from Bitterfeld
FEMS Microbiol. Ecol. 72 (2), 297 - 310 10.1111/j.1574-6941.2010.00845.x
Factors controlling the carbon isotope fractionation of tetra- and trichloroethene during reductive dechlorination by Sulfurospirillum ssp. and Desulfitobacterium sp. strain PCE-S
FEMS Microbiol. Ecol. 62 (1), 98 - 107 10.1111/j.1574-6941.2007.00367.x
Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition
Microb. Biotechnol. 3 (2), 201 - 209 10.1111/j.1751-7915.2009.00124.X
Anaerobically grown Thauera aromatica, Desulfococcus multivorans, Geobacter sulfurreducens are more sensitive towards organic solvents than aerobic bacteria
Appl. Microbiol. Biotechnol. 77 (3), 705 - 711 10.1007/s00253-007-1179-2
Using compound-specific isotope analysis to assess the degradation of chloroacetanilide herbicides in lab-scale wetlands
Chemosphere 99 , 89 - 95 10.1016/j.chemosphere.2013.10.027
Microbial dehalogenation of trichlorinated dibenzo-p-dioxins by a Dehalococcoides-containing mixed culture is coupled to carbon isotope fractionation
Environ. Sci. Technol. 41 (22), 7744 - 7751 10.1021/es070935g
Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants
Environ. Sci. Technol. 38 (7), 2075 - 2081 10.1021/es034989b
Origin of VC-only plumes from naturally enhanced dechlorination in a peat-rich hydrogeologic setting
J. Contam. Hydrol. 192 , 129 - 139 10.1016/j.jconhyd.2016.07.003
Multi-element compound specific stable isotope analysis of chlorinated aliphatic contaminants derived from chlorinated pitches
Sci. Total Environ. 640-641 , 153 - 162 10.1016/j.scitotenv.2018.05.285
Evaluation of aquifer recharge and vulnerability in an alluvial lowland using environmental tracers
J. Hydrol. 529 , 1657 - 1668 10.1016/j.jhydrol.2015.07.055
Stable carbon isotope fractionation of 1,2-Dichloropropane during dichloroelimination by Dehalococcoides populations
Environ. Sci. Technol. 43 (18), 6915 - 6919 10.1021/es900365x
Stable carbon isotope enrichment factors for cis-1,2-dichloroethene and vinyl chloride reductive dechlorination by Dehalococcoides
Environ. Sci. Technol. 45 (7), 2951 - 2957 10.1021/es103728q
Liquid chromatography/isotope ratio mass spectrometry analysis of halogenated benzoates for characterization of the underlying degradation reaction in Thauera chlorobenzoica CB‐1T
Rapid Commun. Mass Spectrom. 32 (11), 906 - 912 10.1002/rcm.8113
Triple-element compound-specific stable isotope analysis of 1,2-dichloroethane for characterization of the underlying dehalogenation reaction in two Dehalococcoides mccartyi strains
FEMS Microbiol. Ecol. 93 (12), fix137 10.1093/femsec/fix137
Dual element (C/Cl) isotope analysis indicates distinct mechanisms of reductive dehalogenation of chlorinated ethenes and dichloroethane in Dehalococcoides mccartyi strain BTF08 with defined reductive dehalogenase inventories
Front. Microbiol. 11 , art. 1507 10.3389/fmicb.2020.01507
Requirements for chromium reactors for use in the determination of H isotopes in compound-specific stable isotope analysis cf chlorinated compounds
Anal. Chem. 92 (3), 2383 - 2387 10.1021/acs.analchem.9b04737
Photosynthesis‐driven methane production in oxic lake water as an important contributor to methane emission
Limnol. Oceanogr. 65 (12), 2853 - 2865 10.1002/lno.11557
Key factors controlling microbial distribution on a DNAPL source area
Environ. Sci. Pollut. Res. 29 (1), 1508 - 1520 10.1007/s11356-021-15635-2
Combined use of ISCR and biostimulation techniques in incomplete processes of reductive dehalogenation of chlorinated solvents
Sci. Total Environ. 648 , 819 - 829 10.1016/j.scitotenv.2018.08.184
The role of ecotones in the dehalogenation of chloroethenes in alluvial fan aquifers
Environ. Sci. Pollut. Res. 28 (21), 26871 - 26884 10.1007/s11356-021-12538-0
Coupling of a headspace autosampler with a programmed temperature vaporizer for stable carbon and hydrogen isotope analysis of volatile organic compounds at microgram per liter concentrations
Anal. Chem. 87 (2), 951 - 959 10.1021/ac503229e
Special issue on anaerobic biological dehalogenation. Editorial
FEMS Microbiol. Ecol. 94 (6), fiy054 10.1093/femsec/fiy054
Reductive debromination by sponge-associated anaerobic bacteria coupled to carbon isotope fractionation
Int. Biodeterior. Biodegrad. 155 , art. 105093 10.1016/j.ibiod.2020.105093
Characterization of microbial communities in the aqueous phase of a constructed model wetland treating 1,2-dichloroethene-contaminated groundwater
FEMS Microbiol. Ecol. 72 (1), 74 - 88 10.1111/j.1574-6941.2009.00825.x
Assessment of in situ degradation of chlorinated ethenes and bacterial community structure in a complex contaminated groundwater system
Water Res. 42 (4-5), 871 - 882 10.1016/j.watres.2007.08.035
Characterization of groundwater microbial communities, dechlorinating bacteria, and in situ biodegradation of chloroethenes along a vertical gradient
Water Air Soil Pollut. 221 (1-4), 107 - 122 10.1007/s11270-011-0774-0
Simulation of dual carbon–bromine stable isotope fractionation during 1,2-dibromoethane degradation
Isot. Environ. Health Stud. 54 (4), 418 - 434 10.1080/10256016.2018.1468759
Isotopic fractionation indicates anaerobic monochlorobenzene biodegradation
Environ. Toxicol. Chem. 24 (6), 1315 - 1324 10.1897/04-321R.1
Assessment of microbial in situ activity in contaminated aquifers
Eng. Life Sci. 6 (3), 234 - 251 10.1002/elsc.200620125
Dehalogenation of diverse halogenated substrates by a highly enriched Dehalococcoides-containing culture derived from the contaminated mega-site in Bitterfeld
FEMS Microbiol. Ecol. 83 (1), 176 - 188 10.1111/j.1574-6941.2012.01462.x
Editorial: Thematic issue on anaerobic biological dehalogenation
FEMS Microbiol. Ecol. 98 (11), fiac108 10.1093/femsec/fiac108
Degradation of aromatic compounds coupled to selenate reduction
Geomicrobiol. J. 19 (1), 77 - 86 10.1080/014904502317246183
Identification of TCE and PCE sorption and biodegradation parameters in a sandy aquifer for fate and transport modelling: batch and column studies
Environ. Sci. Pollut. Res. 22 (13), 9877 - 9888 10.1007/s11356-015-4156-9
Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene
ISME J. 15 (6), 1794 - 1809 10.1038/s41396-020-00887-6
Dual carbon–bromine stable isotope analysis allows distinguishing transformation pathways of ethylene dibromide
Environ. Sci. Technol. 50 (18), 9855 - 9863 10.1021/acs.est.6b01692
Characterisation of a manganese-reducing, toluene-degrading enrichment culture
FEMS Microbiol. Ecol. 24 (2), 113 - 125 10.1111/j.1574-6941.1997.tb00428.x
Biotransformation and inhibition effects of hexachlorocyclohexanes during biogas production from contaminated biomass characterized by isotope fractionation concepts
Bioresour. Technol. 250 , 683 - 690 10.1016/j.biortech.2017.11.076
Biotransformation of hexachlorocyclohexanes contaminated biomass for energetic utilization demonstrated in continuous anaerobic digestion system
J. Hazard. Mater. 384 , art. 121448 10.1016/j.jhazmat.2019.121448
Mechanistic dichotomy in bacterial trichloroethene dechlorination revealed by carbon and chlorine isotope effects
Environ. Sci. Technol. 53 (8), 4245 - 4254 10.1021/acs.est.8b06643
Carbon isotope fractionation during dechlorination of 1,2,3,4-tetrachlorodibenzo-p-dioxin by a Dehalococcoides-containing culture
Chemosphere 80 (10), 1113 - 1119 10.1016/j.chemosphere.2010.06.027
Compound specific and enantioselective stable isotope analysis as tools to monitor transformation of hexachlorocyclohexane (HCH) in a complex aquifer system
Environ. Sci. Technol. 51 (16), 8909 - 8916 10.1021/acs.est.6b05632
Characterization of hexachlorocyclohexane isomer dehydrochlorination by LinA1 and LinA2 using multi-element compound-specific stable isotope analysis
Environ. Sci. Technol. 56 (23), 16848 - 16856 10.1021/acs.est.2c05334
Dual C–Cl isotope analysis for characterizing the anaerobic transformation of α, β, γ, and δ-hexachlorocyclohexane in contaminated aquifers
Water Res. 184 , art. 116128 10.1016/j.watres.2020.116128
Dual C–Cl isotope analysis for characterizing the reductive dechlorination of α- and γ-hexachlorocyclohexane by two Dehalococcoides mccartyi strains and an enrichment culture
Environ. Sci. Technol. 54 (12), 7250 - 7260 10.1021/acs.est.9b06407
Enantiomer and carbon isotope fractionation of α-hexachlorocyclohexane by Sphingobium indicum strain B90A and the corresponding enzymes
Environ. Sci. Technol. 53 (15), 8715 - 8724 10.1021/acs.est.9b01233
Aerobic toluene degraders in the rhizosphere of a constructed wetland model show diurnal polyhydroxyalkanoate metabolism
Appl. Environ. Microb. 82 (14), 4126 - 4132 10.1128/AEM.00493-16
Transformation and carbon isotope fractionation of tetra- and trichloroethene to Trans-Dichloroethene by Dehalococcoides sp. strain CBDB1
Environ. Sci. Technol. 45 (4), 1555 - 1562 10.1021/es1023459
Microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway
Appl. Environ. Microb. 81 (18), 6241 - 6252 10.1128/AEM.01822-15
Detection of monochlorobenzene metabolizing bacteria under anoxic conditions by DNA-stable isotope probing
Biodegradation 22 (5), 973 - 982 10.1007/s10532-011-9456-2
High stability and fast recovery of expression of the TOL plasmid-carried toluene catabolism genes of Pseudomonas putida mt-2 under conditions of oxygen limitation and oscillation
Appl. Environ. Microb. 76 (20), 6715 - 6723 10.1128/AEM.01039-10
Reductive dechlorination of cis-1,2-dichlorethene and vinyl chloride by "Dehalococcoides ethenogenes"
Environ. Sci. Technol. 35 (3), 516 - 521 10.1021/es001285i
Mucosal-associated invariant T-Cell (MAIT) activation is altered by chlorpyrifos- and glyphosate-treated commensal gut bacteria
J. Immunotoxicol. 17 (1), 10 - 20 10.1080/1547691X.2019.1706672
Occurrence of Dehalococcoides and reductive dehalogenase genes in microcosms, a constructed wetland and groundwater from a chlorinated ethene contaminated field site as indicators for in situ reductive dehalogenation
Water Air Soil Pollut. 224 (11), art. 1768 10.1007/s11270-013-1768-x
Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains
J. Hazard. Mater. 331 , 218 - 225 10.1016/j.jhazmat.2017.02.043
Stable isotope fractionation of tetrachloroethene during reductive dechlorination by Sulfurospirillum multivorans and Desulfitobacterium sp. strain PCE-S and abiotic reactions with cyanocobalamin
Appl. Environ. Microb. 71 (7), 3413 - 3419 10.1128/aem.71.7.3413-3419.2005
Use of stable isotope fractionation for the assessment of bioattenuation of chlorinated ethenes
In: Verstraete, W. (ed.)
European Symposium on Environmental Biotechnology : proceedings of the European Symposium on Environmental Biotechnology, ESEB 2004, 25-28 April 2004, Oostende, Belgium
Balkema, Leiden, p. 209 - 212
Anaerobic microbial dehalogenation of organohalides — state of the art and remediation strategies
Curr. Opin. Biotechnol. 38 , 33 - 38 10.1016/j.copbio.2015.11.009
In situ investigation of microbial diversity and anaerobic chlorobenzene degradation along a vertical gradient
Geochim. Cosmochim. Acta 73 (13 Suppl.), A943 10.1016/j.gca.2009.05.024
Assessment of the natural attenuation of chlorinated ethenes in an anaerobic contaminated aquifer in the Bitterfeld/Wolfen area using stable isotope techniques, microcosm studies and molecular biomarkers
Chemosphere 67 (2), 300 - 311 10.1016/j.chemosphere.2006.09.084
Recent advances in multi-element compound-specific stable isotope analysis of organohalides: Achievements, challenges and prospects for assessing environmental sources and transformation
Trends Environ. Anal. Chem. 11 , 1 - 8 10.1016/j.teac.2016.04.001
Stable isotope fractionation concepts for characterizing biotransformation of organohalides
Curr. Opin. Biotechnol. 41 , 108 - 113 10.1016/j.copbio.2016.06.002
A stable isotope approach for source apportionment of chlorinated ethene plumes at a complex multi-contamination events urban site
J. Contam. Hydrol. 153 , 92 - 105 10.1016/j.jconhyd.2013.06.004
Sensitive detection of anaerobic monochlorobenzene degradation using stable isotope tracers
Environ. Sci. Technol. 41 (11), 3836 - 3842 10.1021/es0621896
Anaerobic microbial dehalogenation and its key players in the contaminated Bitterfeld-Wolfen megasite
FEMS Microbiol. Ecol. 94 (4), fiy012 10.1093/femsec/fiy012
Evidence for in situ natural attenuation of monochlorobenzene and chlorinated ethenes in an anaerobic contaminated aquifer
Abstr. Paper Am. Chem. Soc. 228 , U608 - U609
Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195
Appl. Environ. Microb. 71 (3), 1664 - 1667 10.1128/AEM.71.3.1664-1667.2005
Molecular characterization of dichloromethane-degrading Hyphomicrobium strains using 16S rDNA and DCM dehalogenase gene sequences
Syst. Appl. Microbiol. 28 (7), 582 - 587 10.1016/j.syapm.2005.03.011
Stable carbon isotope fractionation during degradation of dichloromethane by methylotrophic bacteria
Environ. Microbiol. 8 (1), 156 - 164 10.1111/j.1462-2920.2005.00878.x
Metagenomic- and cultivation-based exploration of anaerobic chloroform biotransformation in hypersaline sediments as natural source of chloromethanes
Microorganisms 8 (5), art. 665 10.3390/microorganisms8050665
Investigation of active site amino acid influence on carbon and chlorine isotope fractionation during reductive dechlorination
FEMS Microbiol. Ecol. 98 (8), fiac072 10.1093/femsec/fiac072
Genome sequences of two dehalogenation specialists - Dehalococcoides mccartyi strains BTF08 and DCMB5 enriched from the highly polluted Bitterfeld region
FEMS Microbiol. Lett. 343 (2), 101 - 104 10.1111/1574-6968.12160
Dehalococcoides mccartyi strain DCMB5 respires a broad spectrum of chlorinated aromatic compounds
Appl. Environ. Microb. 81 (2), 587 - 596 10.1128/AEM.02597-14
Reductive dechlorination in recalcitrant sources of chloroethenes in the transition zone between aquifers and aquitards
Environ. Sci. Pollut. Res. 23 (18), 18724 - 18741 10.1007/s11356-016-7068-4
Development and validation of an universal interface for compound-specific stable isotope analysis of chlorine (37Cl/35Cl) by GC-high-temperature conversion (HTC)-MS/IRMS
Anal. Chem. 87 (5), 2832 - 2839 10.1021/ac504232u
Combined C and Cl isotope effects indicate differences between corrinoids and enzyme (Sulfurospirillum multivorans PceA) in reductive dehalogenation of tetrachloroethene, but not trichloroethene
Environ. Sci. Technol. 48 (20), 11837 - 11845 10.1021/es503306g
Evaluation of the microbial reductive dehalogenation reaction using compound-specific stable isotope analysis (CSIA)
In: Adrian, L., Löffler, F.E. (eds.)
Organohalide-respiring bacteria
Springer, Berlin, Heidelberg, p. 429 - 453 10.1007/978-3-662-49875-0_18
Substrate hydrophobicity and cell composition influence the extent of rate limitation and masking of isotope fractionation during microbial reductive dehalogenation of chlorinated ethenes
Environ. Sci. Technol. 49 (7), 4293 - 4301 10.1021/es506108j
Comparison of 1,2-dichloroethane, dichloroethene and vinyl chloride carbon stable isotope fractionation during dechlorination by two Dehalococcoides strains
Water Res. 52 , 146 - 154 10.1016/j.watres.2013.12.042
Iron oxides stimulate microbial monochlorobenzene in situ transformation in constructed wetlands and laboratory systems
Sci. Total Environ. 472 , 185 - 193 10.1016/j.scitotenv.2013.10.116
Evaluation of carbon isotope fractionation during anaerobic reductive dehalogenation of chlorinated and brominated benzenes
Chemosphere 193 , 785 - 792 10.1016/j.chemosphere.2017.11.089
In situ microcosms to evaluate natural attenuation potentials in contaminated aquifers
Org. Geochem. 37 (10), 1394 - 1410 10.1016/j.orggeochem.2006.05.011
Integrative approach to delineate natural attenuation of chlorinated benzenes in anoxic aquifers
Environ. Pollut. 157 (6), 1800 - 1806 10.1016/j.envpol.2009.01.029
Combined chemical and microbiological degradation of tetrachloroethene during the application of Carbo-Iron at a contaminated field site
Sci. Total Environ. 628-629 , 1027 - 1036 10.1016/j.scitotenv.2018.01.310
Variable dual carbon-bromine stable isotope fractionation during enzyme-catalyzed reductive dehalogenation of brominated ethenes
Chemosphere 190 , 211 - 217 10.1016/j.chemosphere.2017.09.128
Compound specific stable isotope analysis (CSIA) to characterize transformation mechanisms of α-hexachlorocyclohexane
J. Hazard. Mater. 280 , 750 - 757 10.1016/j.jhazmat.2014.08.046
Anaerobic biotransformation of hexachlorocyclohexane isomers in aqueous condition: dual C-Cl isotope fractionation and impact on microbial community compositions
Water Res. 254 , art. 121389 10.1016/j.watres.2024.121389