Dr. Ivonne Nijenhuis

Curriculum Vitae

Professional & Research interests

My research in the Department of technical Biogeochemistry focuses on the anaerobic biotransformation of halogenated organic contaminants and the application of stable isotope concepts. Currently, these are applied in the BMBF funded project KONATES, addressing the question on how aquifer thermal energy storage affects the in situ biotransformation of halogenated groundwater contaminants, i.e. chlorinated ethenes.

My main expertise include 1) understanding of microbial transformation of chlorinated and brominated ethenes and ethanes, halogenated pesticides, e.g. hexachlorocyclohexane, and pharmaceuticals, e.g. diclofenac, and 2) development and application of multi-element compound-specific stable isotope (C, Cl, Br, H) fractionation as a tool to analyse microbial reactions and biotransformation in situ.

Projects

2011-2019 the DFG Forschergruppe "FOR1530 'Anaerobic Dehalogenation: Organisms, Biochemistry and (Eco-) Physiology'" links research groups in Jena, Halle and Leipzig. Within this project, I was in charge of sub-project 05: 'Characterisation of microbial dehalogenation using compound specific stable isotope analysis'.

Within the European Union FP7 project "GENESIS" we investigated how biogeochemical processes affect natural attenuation of chlorinated benzenes and are focusing on the microbial community involved in contaminant biotransformation.

From 2010-2015, I coordinated the European Union FP7 'People' programme Initial Training Network "CSI:ENVIRONMENT". In this project, 10 Partners and 4 Associated Partners from 9 European countries will work on 'Isotope forensics meets biogeochemistry - linking sources and sinks of organic contaminants by compound specific isotope investigation'.

2012-2014, Joint German-Israeli Water Technology Programme “Development of innovative tools for the assessment of in situ biodegradation of organobromine contaminant in groundwater” – INTIME. In this 3 year project we will investigate the application of CSIA for assessment of the natural attenuation of brominated groundwater contaminants.

Publications (in co-operation with the UFZ)

• Bashir, S., Fischer, A., Nijenhuis, I., Richnow, H.H. (2013):
  Enantioselective carbon stable isotope fractionation of hexachlorocyclohexane during aerobic biodegradation by Sphingobium spp.
  Environ. Sci. Technol. 47 (20), 11432 - 11439 10.1021/es402197s
• Bashir, S., Kuntze, K., Vogt, C., Nijenhuis, I. (2018):
  Anaerobic biotransformation of hexachlorocyclohexane isomers by Dehalococcoides species and an enrichment culture
  Biodegradation 29 (4), 409 - 418 10.1007/s10532-018-9838-9
• Bin Hudari, M.S., Richnow, H., Vogt, C., Nijenhuis, I. (2022):
  Effect of temperature on microbial reductive dehalogenation of chlorinated ethenes: a review
  FEMS Microbiol. Ecol. 98 (9), fiac081 10.1093/femsec/fiac081
• Birkigt, J., Gilevska, T., Ricken, B., Richnow, H.-H., Vione, D., Corvini, P.F.-X., Nijenhuis, I., Cichocka, D. (2015):
  Carbon stable isotope fractionation of sulfamethoxazole during biodegradation by Microbacterium sp. strain BR1 and upon direct photolysis
  Environ. Sci. Technol. 49 (10), 6029 - 6036 10.1021/acs.est.5b00367
• Birkigt, J., Stumpp, C., Małoszewski, P., Nijenhuis, I. (2018):
  Evaluation of the hydrological flow paths in a gravel bed filter modeling a horizontal subsurface flow wetland by using a multi-tracer experiment
  Sci. Total Environ. 621 , 265 - 272 10.1016/j.scitotenv.2017.11.217
• Cichocka, D., Imfeld, G., Richnow, H.H., Nijenhuis, I. (2008):
  Variability in microbial carbon isotope fractionation of tetra- and trichloroethene upon reductive dechlorination
  Chemosphere 71 (4), 639 - 648 10.1016/j.chemosphere.2007.11.013
• Cichocka, D., Nikolausz, M., Haest, P.J., Nijenhuis, I. (2010):
  Tetrachloroethene conversion to ethene by a Dehalococcoides-containing enrichment culture from Bitterfeld
  FEMS Microbiol. Ecol. 72 (2), 297 - 310 10.1111/j.1574-6941.2010.00845.x
• Cichocka, D., Siegert, M., Imfeld, G., Andert, J., Beck, K., Diekert, G., Richnow, H.H., Nijenhuis, I. (2007):
  Factors controlling the carbon isotope fractionation of tetra- and trichloroethene during reductive dechlorination by Sulfurospirillum ssp. and Desulfitobacterium sp. strain PCE-S
  FEMS Microbiol. Ecol. 62 (1), 98 - 107 10.1111/j.1574-6941.2007.00367.x
• Duldhardt, I., Gaebel, J., Chrzanowski, Ł., Nijenhuis, I., Schauer, F., Heipieper, H.J. (2010):
  Adaptation of anaerobically grown Thauera aromatica, Geobacter sulfurreducens and Desulfococcus multivorans to organic solvents on the level of membrane fatty acid composition
  Microb. Biotechnol. 3 (2), 201 - 209 10.1111/j.1751-7915.2009.00124.X
• Duldhardt, I., Nijenhuis, I., Schauer, F., Heipieper, H.J. (2007):
  Anaerobically grown Thauera aromatica, Desulfococcus multivorans, Geobacter sulfurreducens are more sensitive towards organic solvents than aerobic bacteria
  Appl. Microbiol. Biotechnol. 77 (3), 705 - 711 10.1007/s00253-007-1179-2
• Elsayed, O.F., Maillard, E., Vuilleumier, S., Nijenhuis, I., Richnow, H.H., Imfeld, G. (2014):
  Using compound-specific isotope analysis to assess the degradation of chloroacetanilide herbicides in lab-scale wetlands
  Chemosphere 99 , 89 - 95 10.1016/j.chemosphere.2013.10.027
• Ewald, E.-M., Wagner, A., Nijenhuis, I., Richnow, H.H., Lechner, U. (2007):
  Microbial dehalogenation of trichlorinated dibenzo-p-dioxins by a Dehalococcoides-containing mixed culture is coupled to carbon isotope fractionation
  Environ. Sci. Technol. 41 (22), 7744 - 7751 10.1021/es070935g
• Fennell, D.E., Nijenhuis, I., Wilson, S.F., Zinder, S.H., Häggblom, M.M. (2004):
  Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants
  Environ. Sci. Technol. 38 (7), 2075 - 2081 10.1021/es034989b
• Filippini, M., Amorosi, A., Campo, B., Herrero-Martìn, S., Nijenhuis, I., Parker, B.L., Gargini, A. (2016):
  Origin of VC-only plumes from naturally enhanced dechlorination in a peat-rich hydrogeologic setting
  J. Contam. Hydrol. 192 , 129 - 139 10.1016/j.jconhyd.2016.07.003
• Filippini, M., Nijenhuis, I., Kümmel, S., Chiarini, V., Crosta, G., Richnow, H.H., Gargini, A. (2018):
  Multi-element compound specific stable isotope analysis of chlorinated aliphatic contaminants derived from chlorinated pitches
  Sci. Total Environ. 640-641 , 153 - 162 10.1016/j.scitotenv.2018.05.285
• Filippini, M., Stumpp, C., Nijenhuis, I., Richnow, H.H., Gargini, A. (2015):
  Evaluation of aquifer recharge and vulnerability in an alluvial lowland using environmental tracers
  J. Hydrol. 529 , 1657 - 1668 10.1016/j.jhydrol.2015.07.055
• Fletcher, K.E., Löffler, F.E., Richnow, H.-H., Nijenhuis, I. (2009):
  Stable carbon isotope fractionation of 1,2-Dichloropropane during dichloroelimination by Dehalococcoides populations
  Environ. Sci. Technol. 43 (18), 6915 - 6919 10.1021/es900365x
• Fletcher, K.E., Nijenhuis, I., Richnow, H.-H., Löffler, F.E. (2011):
  Stable carbon isotope enrichment factors for cis-1,2-dichloroethene and vinyl chloride reductive dechlorination by Dehalococcoides
  Environ. Sci. Technol. 45 (7), 2951 - 2957 10.1021/es103728q
• Franke, S., Kümmel, S., Nijenhuis, I. (2018):
  Liquid chromatography/isotope ratio mass spectrometry analysis of halogenated benzoates for characterization of the underlying degradation reaction in Thauera chlorobenzoica CB‐1^T
  Rapid Commun. Mass Spectrom. 32 (11), 906 - 912 10.1002/rcm.8113
• Franke, S., Lihl, C., Renpenning, J., Elsner, M., Nijenhuis, I. (2017):
  Triple-element compound-specific stable isotope analysis of 1,2-dichloroethane for characterization of the underlying dehalogenation reaction in two Dehalococcoides mccartyi strains
  FEMS Microbiol. Ecol. 93 (12), fix137 10.1093/femsec/fix137
• Franke, S., Seidel, K., Adrian, L., Nijenhuis, I. (2020):
  Dual element (C/Cl) isotope analysis indicates distinct mechanisms of reductive dehalogenation of chlorinated ethenes and dichloroethane in Dehalococcoides mccartyi strain BTF08 with defined reductive dehalogenase inventories
  Front. Microbiol. 11 , art. 1507 10.3389/fmicb.2020.01507
• Gilevska, T., Sullivan Ojeda, A., Renpenning, J., Kümmel, S., Gehre, M., Nijenhuis, I. (2020):
  Requirements for chromium reactors for use in the determination of H isotopes in compound-specific stable isotope analysis cf chlorinated compounds
  Anal. Chem. 92 (3), 2383 - 2387 10.1021/acs.analchem.9b04737
• Günthel, M., Klawonn, I., Woodhouse, J., Bižić, M., Ionescu, D., Ganzert, L., Kümmel, S., Nijenhuis, I., Zoccarato, L., Grossart, H.-P., Tang, K.W. (2020):
  Photosynthesis‐driven methane production in oxic lake water as an important contributor to methane emission
  Limnol. Oceanogr. 65 (12), 2853 - 2865 10.1002/lno.11557
• Herrero, J., Puigserver, D., Nijenhuis, I., Kuntze, K., Carmona, J.M. (2022):
  Key factors controlling microbial distribution on a DNAPL source area
  Environ. Sci. Pollut. Res. 29 (1), 1508 - 1520 10.1007/s11356-021-15635-2
• Herrero, J., Puigserver, D., Nijenhuis, I., Kuntze, K., Carmona, J.M. (2019):
  Combined use of ISCR and biostimulation techniques in incomplete processes of reductive dehalogenation of chlorinated solvents
  Sci. Total Environ. 648 , 819 - 829 10.1016/j.scitotenv.2018.08.184
• Herrero, J., Puigserver, D., Nijenhuis, I., Kuntze, K., Parker, B.L., Carmona, J.M. (2021):
  The role of ecotones in the dehalogenation of chloroethenes in alluvial fan aquifers
  Environ. Sci. Pollut. Res. 28 (21), 26871 - 26884 10.1007/s11356-021-12538-0
• Herrero-Martín, S., Nijenhuis, I., Richnow, H.H., Gehre, M. (2015):
  Coupling of a headspace autosampler with a programmed temperature vaporizer for stable carbon and hydrogen isotope analysis of volatile organic compounds at microgram per liter concentrations
  Anal. Chem. 87 (2), 951 - 959 10.1021/ac503229e
• Holliger, C., Nijenhuis, I. (2018):
  Special issue on anaerobic biological dehalogenation. Editorial
  FEMS Microbiol. Ecol. 94 (6), fiy054 10.1093/femsec/fiy054
• Horna-Gray, I., Lopez, N.A., Nijenhuis, I., Ahn, Y., Richnow, H.H., Häggblom, M.M. (2020):
  Reductive debromination by sponge-associated anaerobic bacteria coupled to carbon isotope fractionation
  Int. Biodeterior. Biodegrad. 155 , art. 105093 10.1016/j.ibiod.2020.105093
• Imfeld, G., Aragonés, C.E., Fetzer, I., Mészáros, E., Zeiger, S., Nijenhuis, I., Nikolausz, M., Delerce, S., Richnow, H.H. (2010):
  Characterization of microbial communities in the aqueous phase of a constructed model wetland treating 1,2-dichloroethene-contaminated groundwater
  FEMS Microbiol. Ecol. 72 (1), 74 - 88 10.1111/j.1574-6941.2009.00825.x
• Imfeld, G., Nijenhuis, I., Nikolausz, M., Zeiger, S., Paschke, H., Drangmeister, J., Grossmann, J., Richnow, H.H., Weber, S. (2008):
  Assessment of in situ degradation of chlorinated ethenes and bacterial community structure in a complex contaminated groundwater system
  Water Res. 42 (4-5), 871 - 882 10.1016/j.watres.2007.08.035
• Imfeld, G., Pieper, H., Shani, N., Rossi, P., Nikolausz, M., Nijenhuis, I., Paschke, H., Weiß, H., Richnow, H.H. (2011):
  Characterization of groundwater microbial communities, dechlorinating bacteria, and in situ biodegradation of chloroethenes along a vertical gradient
  Water Air Soil Pollut. 221 (1-4), 107 - 122 10.1007/s11270-011-0774-0
• Jin, B., Nijenhuis, I., Rolle, M. (2018):
  Simulation of dual carbon–bromine stable isotope fractionation during 1,2-dibromoethane degradation
  Isot. Environ. Health Stud. 54 (4), 418 - 434 10.1080/10256016.2018.1468759
• Kaschl, A., Vogt, C., Uhlig, S., Nijenhuis, I., Weiß, H., Kästner, M., Richnow, H.H. (2005):
  Isotopic fractionation indicates anaerobic monochlorobenzene biodegradation
  Environ. Toxicol. Chem. 24 (6), 1315 - 1324 10.1897/04-321R.1
• Kästner, M., Fischer, A., Nijenhuis, I., Geyer, R., Stelzer, N., Bombach, P., Tebbe, C.C., Richnow, H.H. (2006):
  Assessment of microbial in situ activity in contaminated aquifers
  Eng. Life Sci. 6 (3), 234 - 251 10.1002/elsc.200620125
• Kaufhold, T., Schmidt, M., Cichocka, D., Nikolausz, M., Nijenhuis, I. (2013):
  Dehalogenation of diverse halogenated substrates by a highly enriched Dehalococcoides-containing culture derived from the contaminated mega-site in Bitterfeld
  FEMS Microbiol. Ecol. 83 (1), 176 - 188 10.1111/j.1574-6941.2012.01462.x
• Khan, M.I., Yoo, K., Schwab, L., Kümmel, S., Nijenhuis, I. (2024):
  Characterization of anaerobic biotransformation of hexachlorocyclohexanes by novel microbial consortia enriched from channel and river sediments
  J. Hazard. Mater. 476 , art. 135198 10.1016/j.jhazmat.2024.135198
• Kjellerup, B.V., Nijenhuis, I., Rossetti, S. (2022):
  Editorial: Thematic issue on anaerobic biological dehalogenation
  FEMS Microbiol. Ecol. 98 (11), fiac108 10.1093/femsec/fiac108
• Knight, V.K., Nijenhuis, I., Kerkhof, L.J., Häggblom, M.M. (2002):
  Degradation of aromatic compounds coupled to selenate reduction
  Geomicrobiol. J. 19 (1), 77 - 86 10.1080/014904502317246183
• Kret, E., Kiecak, A., Malina, G., Nijenhuis, I., Postawa, A. (2015):
  Identification of TCE and PCE sorption and biodegradation parameters in a sandy aquifer for fate and transport modelling: batch and column studies
  Environ. Sci. Pollut. Res. 22 (13), 9877 - 9888 10.1007/s11356-015-4156-9
• Kruse, S., Türkowsky, D., Birkigt, J., Matturro, B., Franke, S., Jehmlich, N., von Bergen, M., Westermann, M., Rossetti, S., Nijenhuis, I., Adrian, L., Diekert, G., Goris, T. (2021):
  Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene
  ISME J. 15 (6), 1794 - 1809 10.1038/s41396-020-00887-6
• Kuntze, K., Kozell, A., Richnow, H.H., Halicz, L., Nijenhuis, I., Gelman, F. (2016):
  Dual carbon–bromine stable isotope analysis allows distinguishing transformation pathways of ethylene dibromide
  Environ. Sci. Technol. 50 (18), 9855 - 9863 10.1021/acs.est.6b01692
• Langenhoff, A., Nijenhuis, I., Tan, N.C.G., Briglia, M., Zehnder, A.J.B., Schraa, G. (1997):
  Characterisation of a manganese-reducing, toluene-degrading enrichment culture
  FEMS Microbiol. Ecol. 24 (2), 113 - 125 10.1111/j.1574-6941.1997.tb00428.x
• Lian, S., Nikolausz, M., Nijenhuis, I., Leite, A.F., Richnow, H.H. (2018):
  Biotransformation and inhibition effects of hexachlorocyclohexanes during biogas production from contaminated biomass characterized by isotope fractionation concepts
  Bioresour. Technol. 250 , 683 - 690 10.1016/j.biortech.2017.11.076
• Lian, S., Nikolausz, M., Nijenhuis, I., Nunes da Rocha, U., Liu, B., Borim Corrêa, F., Saraiva, J.P., Richnow, H.H. (2020):
  Biotransformation of hexachlorocyclohexanes contaminated biomass for energetic utilization demonstrated in continuous anaerobic digestion system
  J. Hazard. Mater. 384 , art. 121448 10.1016/j.jhazmat.2019.121448
• Lihl, C., Douglas, L.M., Franke, S., Pérez-de-Mora, A., Meyer, A.H., Daubmeier, M., Edwards, E.A., Nijenhuis, I., Sherwood Lollar, B., Elsner, M. (2019):
  Mechanistic dichotomy in bacterial trichloroethene dechlorination revealed by carbon and chlorine isotope effects
  Environ. Sci. Technol. 53 (8), 4245 - 4254 10.1021/acs.est.8b06643
• Liu, F., Cichocka, D., Nijenhuis, I., Richnow, H.H., Fennell, D.E. (2010):
  Carbon isotope fractionation during dechlorination of 1,2,3,4-tetrachlorodibenzo-p-dioxin by a Dehalococcoides-containing culture
  Chemosphere 80 (10), 1113 - 1119 10.1016/j.chemosphere.2010.06.027
• Liu, Y., Bashir, S., Stollberg, R., Trabitzsch, R., Weiß, H., Paschke, H., Nijenhuis, I., Richnow, H.-H. (2017):
  Compound specific and enantioselective stable isotope analysis as tools to monitor transformation of hexachlorocyclohexane (HCH) in a complex aquifer system
  Environ. Sci. Technol. 51 (16), 8909 - 8916 10.1021/acs.est.6b05632
• Liu, Y., Fu, J., Wu, L., Kümmel, S., Nijenhuis, I., Richnow, H.H. (2022):
  Characterization of hexachlorocyclohexane isomer dehydrochlorination by LinA1 and LinA2 using multi-element compound-specific stable isotope analysis
  Environ. Sci. Technol. 56 (23), 16848 - 16856 10.1021/acs.est.2c05334
• Liu, Y., Kümmel, S., Yao, J., Nijenhuis, I., Richnow, H.-H. (2020):
  Dual C–Cl isotope analysis for characterizing the anaerobic transformation of α, β, γ, and δ-hexachlorocyclohexane in contaminated aquifers
  Water Res. 184 , art. 116128 10.1016/j.watres.2020.116128
• Liu, Y., Renpenning, J., Nijenhuis, I., Richnow, H.-H. (2020):
  Dual C–Cl isotope analysis for characterizing the reductive dechlorination of α- and γ-hexachlorocyclohexane by two Dehalococcoides mccartyi strains and an enrichment culture
  Environ. Sci. Technol. 54 (12), 7250 - 7260 10.1021/acs.est.9b06407
• Liu, Y., Wu, L., Kohli, P., Kumar, R., Stryhanyuk, H., Nijenhuis, I., Lal, R., Richnow, H.-H. (2019):
  Enantiomer and carbon isotope fractionation of α-hexachlorocyclohexane by Sphingobium indicum strain B90A and the corresponding enzymes
  Environ. Sci. Technol. 53 (15), 8715 - 8724 10.1021/acs.est.9b01233
• Lünsmann, V., Kappelmeyer, U., Taubert, A., Nijenhuis, I., von Bergen, M., Heipieper, H.J., Müller, J.A., Jehmlich, N. (2016):
  Aerobic toluene degraders in the rhizosphere of a constructed wetland model show diurnal polyhydroxyalkanoate metabolism
  Appl. Environ. Microb. 82 (14), 4126 - 4132 10.1128/AEM.00493-16
• Marco-Urrea, E., Nijenhuis, I., Adrian, L. (2011):
  Transformation and carbon isotope fractionation of tetra- and trichloroethene to Trans-Dichloroethene by Dehalococcoides sp. strain CBDB1
  Environ. Sci. Technol. 45 (4), 1555 - 1562 10.1021/es1023459
• Martínez-Lavanchy, P.M., Chen, Z., Lünsmann, V., Marin-Cevada, V., Vilchez-Vargas, R., Pieper, D.H., Reiche, N., Kappelmeyer, U., Imparato, V., Junca, H., Nijenhuis, I., Müller, J.A., Kuschk, P., Heipieper, H.J. (2015):
  Microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway
  Appl. Environ. Microb. 81 (18), 6241 - 6252 10.1128/AEM.01822-15
• Martínez-Lavanchy, P.M., Dohrmann, A.B., Imfeld, G., Trescher, K., Tebbe, C.C., Richnow, H.-H., Nijenhuis, I. (2011):
  Detection of monochlorobenzene metabolizing bacteria under anoxic conditions by DNA-stable isotope probing
  Biodegradation 22 (5), 973 - 982 10.1007/s10532-011-9456-2
• Martínez-Lavanchy, P.M., Müller, C., Nijenhuis, I., Kappelmeyer, U., Buffing, M., McPherson, K., Heipieper, H.J. (2010):
  High stability and fast recovery of expression of the TOL plasmid-carried toluene catabolism genes of Pseudomonas putida mt-2 under conditions of oxygen limitation and oscillation
  Appl. Environ. Microb. 76 (20), 6715 - 6723 10.1128/AEM.01039-10
• Maymó-Gatell, X., Nijenhuis, I., Zinder, S.H. (2001):
  Reductive dechlorination of cis-1,2-dichlorethene and vinyl chloride by "Dehalococcoides ethenogenes"
  Environ. Sci. Technol. 35 (3), 516 - 521 10.1021/es001285i
• Mendler, A., Geier, F., Haange, S.-B., Pierzchalski, A., Krause, J.L., Nijenhuis, I., Froment, J., Jehmlich, N., Berger, U., Ackermann, G., Rolle-Kampczyk, U., von Bergen, M., Herberth, G. (2020):
  Mucosal-associated invariant T-Cell (MAIT) activation is altered by chlorpyrifos- and glyphosate-treated commensal gut bacteria
  J. Immunotoxicol. 17 (1), 10 - 20 10.1080/1547691X.2019.1706672
• Mészáros, E., Imfeld, G., Nikolausz, M., Nijenhuis, I. (2013):
  Occurrence of Dehalococcoides and reductive dehalogenase genes in microcosms, a constructed wetland and groundwater from a chlorinated ethene contaminated field site as indicators for in situ reductive dehalogenation
  Water Air Soil Pollut. 224 (11), art. 1768 10.1007/s11270-013-1768-x
• Mortan, S.H., Martín-González, L., Vicent, T., Caminal, G., Nijenhuis, I., Adrian, L., Marco-Urrea, E. (2017):
  Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains
  J. Hazard. Mater. 331 , 218 - 225 10.1016/j.jhazmat.2017.02.043
• Nijenhuis, I., Andert, J., Beck, K., Kästner, M., Diekert, G., Richnow, H.H. (2005):
  Stable isotope fractionation of tetrachloroethene during reductive dechlorination by Sulfurospirillum multivorans and Desulfitobacterium sp. strain PCE-S and abiotic reactions with cyanocobalamin
  Appl. Environ. Microb. 71 (7), 3413 - 3419 10.1128/aem.71.7.3413-3419.2005
• Nijenhuis, I., Andert, J., Beck, K., Vieth, A., Kästner, M., Richnow, H.H., Diekert, G. (2004):
  Use of stable isotope fractionation for the assessment of bioattenuation of chlorinated ethenes
  In: Verstraete, W. (ed.)
  European Symposium on Environmental Biotechnology : proceedings of the European Symposium on Environmental Biotechnology, ESEB 2004, 25-28 April 2004, Oostende, Belgium
  Balkema, Leiden, p. 209 - 212
• Nijenhuis, I., Kuntze, K. (2016):
  Anaerobic microbial dehalogenation of organohalides — state of the art and remediation strategies
  Curr. Opin. Biotechnol. 38 , 33 - 38 10.1016/j.copbio.2015.11.009
• Nijenhuis, I., Martínez, P., Junca, H., Stelzer, N., Imfeld, G., Weber, S., Richnow, H. (2009):
  In situ investigation of microbial diversity and anaerobic chlorobenzene degradation along a vertical gradient
  Geochim. Cosmochim. Acta 73 (13 Suppl.), A943 10.1016/j.gca.2009.05.024
• Nijenhuis, I., Nikolausz, M., Köth, A., Felföldi, T., Weiß, H., Drangmeister, J., Großmann, J., Kästner, M., Richnow, H.H. (2007):
  Assessment of the natural attenuation of chlorinated ethenes in an anaerobic contaminated aquifer in the Bitterfeld/Wolfen area using stable isotope techniques, microcosm studies and molecular biomarkers
  Chemosphere 67 (2), 300 - 311 10.1016/j.chemosphere.2006.09.084
• Nijenhuis, I., Renpenning, J., Kümmel, S., Richnow, H.H., Gehre, M. (2016):
  Recent advances in multi-element compound-specific stable isotope analysis of organohalides: Achievements, challenges and prospects for assessing environmental sources and transformation
  Trends Environ. Anal. Chem. 11 , 1 - 8 10.1016/j.teac.2016.04.001
• Nijenhuis, I., Richnow, H.H. (2016):
  Stable isotope fractionation concepts for characterizing biotransformation of organohalides
  Curr. Opin. Biotechnol. 41 , 108 - 113 10.1016/j.copbio.2016.06.002
• Nijenhuis, I., Schmidt, M., Pellegatti, E., Paramatti, E., Richnow, H.-H., Gargini, A. (2013):
  A stable isotope approach for source apportionment of chlorinated ethene plumes at a complex multi-contamination events urban site
  J. Contam. Hydrol. 153 , 92 - 105 10.1016/j.jconhyd.2013.06.004
• Nijenhuis, I., Stelzer, N., Kästner, M., Richnow, H.-H. (2007):
  Sensitive detection of anaerobic monochlorobenzene degradation using stable isotope tracers
  Environ. Sci. Technol. 41 (11), 3836 - 3842 10.1021/es0621896
• Nijenhuis, I., Stollberg, R., Lechner, U. (2018):
  Anaerobic microbial dehalogenation and its key players in the contaminated Bitterfeld-Wolfen megasite
  FEMS Microbiol. Ecol. 94 (4), fiy012 10.1093/femsec/fiy012
• Nijenhuis, I., Uhlig, S., Kaschl, A., Nikolausz, M., Kästner, M. (2004):
  Evidence for in situ natural attenuation of monochlorobenzene and chlorinated ethenes in an anaerobic contaminated aquifer
  Abstr. Paper Am. Chem. Soc. 228 , U608 - U609
• Nijenhuis, I., Zinder, S.H. (2005):
  Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195
  Appl. Environ. Microb. 71 (3), 1664 - 1667 10.1128/AEM.71.3.1664-1667.2005
• Nikolausz, M., Kappelmeyer, U., Nijenhuis, I., Ziller, K., Kästner, M. (2005):
  Molecular characterization of dichloromethane-degrading Hyphomicrobium strains using 16S rDNA and DCM dehalogenase gene sequences
  Syst. Appl. Microbiol. 28 (7), 582 - 587 10.1016/j.syapm.2005.03.011
• Nikolausz, M., Nijenhuis, I., Ziller, K., Richnow, H.H., Kästner, M. (2006):
  Stable carbon isotope fractionation during degradation of dichloromethane by methylotrophic bacteria
  Environ. Microbiol. 8 (1), 156 - 164 10.1111/j.1462-2920.2005.00878.x
• Peng, P., Lu, Y., Bosma, T.N.P., Nijenhuis, I., Nijsse, B., Shetty, S.A., Ruecker, A., Umanets, A., Ramiro-Garcia, J., Kappler, A., Sipkema, D., Smidt, H., Atashgahi, S. (2020):
  Metagenomic- and cultivation-based exploration of anaerobic chloroform biotransformation in hypersaline sediments as natural source of chloromethanes
  Microorganisms 8 (5), art. 665 10.3390/microorganisms8050665
• Phillips, E., Bulka, O., Picott, K., Kümmel, S., Edwards, E., Nijenhuis, I., Gehre, M., Dworatzek, S., Webb, J., Sherwood Lollar, B. (2022):
  Investigation of active site amino acid influence on carbon and chlorine isotope fractionation during reductive dechlorination
  FEMS Microbiol. Ecol. 98 (8), fiac072 10.1093/femsec/fiac072
• Phillips, E., Picott, K., Kümmel, S., Bulka, O., Edwards, E., Wang, P.-H., Gehre, M., Nijenhuis, I., Lollar, B.S. (2024):
  Vitamin B₁₂ as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter
  MicrobiologyOpen 13 (4), e1433 10.1002/mbo3.1433
• Pöritz, M., Goris, T., Wubet, T., Tarkka, M.T., Buscot, F., Nijenhuis, I., Lechner, U., Adrian, L. (2013):
  Genome sequences of two dehalogenation specialists - Dehalococcoides mccartyi strains BTF08 and DCMB5 enriched from the highly polluted Bitterfeld region
  FEMS Microbiol. Lett. 343 (2), 101 - 104 10.1111/1574-6968.12160
• Pöritz, M., Schiffmann, C.L., Hause, G., Heinemann, U., Seifert, J., Jehmlich, N., von Bergen, M., Nijenhuis, I., Lechner, U. (2015):
  Dehalococcoides mccartyi strain DCMB5 respires a broad spectrum of chlorinated aromatic compounds
  Appl. Environ. Microb. 81 (2), 587 - 596 10.1128/AEM.02597-14
• Puigserver, S., Herrero, J., Torres, M., Cortés, A., Nijenhuis, I., Kuntze, K., Parker, B.L., Carmona, J.M. (2016):
  Reductive dechlorination in recalcitrant sources of chloroethenes in the transition zone between aquifers and aquitards
  Environ. Sci. Pollut. Res. 23 (18), 18724 - 18741 10.1007/s11356-016-7068-4
• Renpenning, J., Hitzfeld, K.L., Gilevska, T., Nijenhuis, I., Gehre, M., Richnow, H.-H. (2015):
  Development and validation of an universal interface for compound-specific stable isotope analysis of chlorine (³⁷Cl/³⁵Cl) by GC-high-temperature conversion (HTC)-MS/IRMS
  Anal. Chem. 87 (5), 2832 - 2839 10.1021/ac504232u
• Renpenning, J., Keller, S., Cretnik, S., Shouakar-Stash, O., Elsner, M., Schubert, T., Nijenhuis, I. (2014):
  Combined C and Cl isotope effects indicate differences between corrinoids and enzyme (Sulfurospirillum multivorans PceA) in reductive dehalogenation of tetrachloroethene, but not trichloroethene
  Environ. Sci. Technol. 48 (20), 11837 - 11845 10.1021/es503306g
• Renpenning, J., Nijenhuis, I. (2016):
  Evaluation of the microbial reductive dehalogenation reaction using compound-specific stable isotope analysis (CSIA)
  In: Adrian, L., Löffler, F.E. (eds.)
  Organohalide-respiring bacteria
  Springer, Berlin, Heidelberg, p. 429 - 453 10.1007/978-3-662-49875-0_18
• Renpenning, J., Rapp, I., Nijenhuis, I. (2015):
  Substrate hydrophobicity and cell composition influence the extent of rate limitation and masking of isotope fractionation during microbial reductive dehalogenation of chlorinated ethenes
  Environ. Sci. Technol. 49 (7), 4293 - 4301 10.1021/es506108j
• Schmidt, M., Lege, S., Nijenhuis, I. (2014):
  Comparison of 1,2-dichloroethane, dichloroethene and vinyl chloride carbon stable isotope fractionation during dechlorination by two Dehalococcoides strains
  Water Res. 52 , 146 - 154 10.1016/j.watres.2013.12.042
• Schmidt, M., Wolfram, D., Birkigt, J., Ahlheim, J., Paschke, H., Richnow, H.-H., Nijenhuis, I. (2014):
  Iron oxides stimulate microbial monochlorobenzene in situ transformation in constructed wetlands and laboratory systems
  Sci. Total Environ. 472 , 185 - 193 10.1016/j.scitotenv.2013.10.116
• Sohn, S.Y., Kuntze, K., Nijenhuis, I., Häggblom, M.M. (2018):
  Evaluation of carbon isotope fractionation during anaerobic reductive dehalogenation of chlorinated and brominated benzenes
  Chemosphere 193 , 785 - 792 10.1016/j.chemosphere.2017.11.089
• Stelzer, N., Büning, C., Pfeifer, F., Dohrmann, A.B., Tebbe, C.C., Nijenhuis, I., Kästner, M., Richnow, H.H. (2006):
  In situ microcosms to evaluate natural attenuation potentials in contaminated aquifers
  Org. Geochem. 37 (10), 1394 - 1410 10.1016/j.orggeochem.2006.05.011
• Stelzer, N., Imfeld, G., Thullner, M., Lehmann, J., Poser, A., Richnow, H.-H., Nijenhuis, I. (2009):
  Integrative approach to delineate natural attenuation of chlorinated benzenes in anoxic aquifers
  Environ. Pollut. 157 (6), 1800 - 1806 10.1016/j.envpol.2009.01.029
• Vogel, M., Nijenhuis, I., Lloyd, J., Boothman, C., Pöritz, M., Mackenzie, K. (2018):
  Combined chemical and microbiological degradation of tetrachloroethene during the application of Carbo-Iron at a contaminated field site
  Sci. Total Environ. 628-629 , 1027 - 1036 10.1016/j.scitotenv.2018.01.310
• Wang, Z., Yang, Y., Kümmel, S., Richnow, H.-H., Nijenhuis, I., Vogt, C. (2025):
  Heterotrophic nitrate reduction potential of an aquifer microbial community from psychrophilic to thermophilic conditions
  Sci. Total Environ. 967 , art. 178716 10.1016/j.scitotenv.2025.178716
• Wienkenjohann, H., Bin Hudari, M.S., Mosthaf, K., Vogt, C., Nijenhuis, I., Rolle, M. (2025):
  Combining microcosm biodegradation and reactive transport modeling to explore the feasibility of ATES-bioremediation approaches
  Front. Water 7 , art. 1499448 10.3389/frwa.2025.1499448
• Woods, A., Kuntze, K., Gelman, F., Halicz, L., Nijenhuis, I. (2018):
  Variable dual carbon-bromine stable isotope fractionation during enzyme-catalyzed reductive dehalogenation of brominated ethenes
  Chemosphere 190 , 211 - 217 10.1016/j.chemosphere.2017.09.128
• Zhang, N., Bashir, S., Qin, J., Schindelka, J., Fischer, A., Nijenhuis, I., Herrmann, H., Wick, L.Y., Richnow, H.H. (2014):
  Compound specific stable isotope analysis (CSIA) to characterize transformation mechanisms of α-hexachlorocyclohexane
  J. Hazard. Mater. 280 , 750 - 757 10.1016/j.jhazmat.2014.08.046
• Zhang, X., Zheng, Y., Su, Z., Wang, Z., Zhang, J., Jia, Z., Kümmel, S., Qin, C., Liu, Y., Wang, S., Nijenhuis, I., Richnow, H.H. (2024):
  Anaerobic biotransformation of hexachlorocyclohexane isomers in aqueous condition: dual C-Cl isotope fractionation and impact on microbial community compositions
  Water Res. 254 , art. 121389 10.1016/j.watres.2024.121389