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Title (Primary) Enantioselective carbon stable isotope fractionation of hexachlorocyclohexane during aerobic biodegradation by Sphingobium spp.
Author Bashir, S.; Fischer, A.; Nijenhuis, I.; Richnow, H.H.;
Journal Environmental Science & Technology
Year 2013
Department ISOBIO;
Volume 47
Issue 20
Language englisch;
POF III (all) T41;
UFZ wide themes RU3;
Abstract Carbon isotope fractionation was investigated for the biotransformation of γ- and α- hexachlorocyclohexane (HCH) as well as enantiomers of α-HCH using two aerobic bacterial strains: Sphingobium indicum strain B90A and Sphingobium japonicum strain UT26. Carbon isotope enrichment factors (εc) for γ-HCH (εc = −1.5 ± 0.1‰ and −1.7 ± 0.2‰) and α-HCH (εc = −1.0 ± 0.2‰ and −1.6 ± 0.3‰) were similar for both aerobic strains, but lower in comparison with previously reported values for anaerobic γ- and α-HCH degradation. Isotope fractionation of α-HCH enantiomers was higher for (+) α-HCH (εc = −2.4 ± 0.8 ‰ and −3.3 ± 0.8 ‰) in comparison to (−) α-HCH (εc = −0.7 ± 0.2‰ and −1.0 ± 0.6‰). The microbial fractionation between the α-HCH enantiomers was quantified by the Rayleigh equation and enantiomeric fractionation factors (εe) for S. indicum strain B90A and S. japonicum strain UT26 were −42 ± 16% and −22 ± 6%, respectively. The extent and range of isomer and enantiomeric carbon isotope fractionation of HCHs with Sphingobium spp. suggests that aerobic biodegradation of HCHs can be monitored in situ by compound-specific stable isotope analysis (CSIA) and enantiomer-specific isotope analysis (ESIA). In addition, enantiomeric fractionation has the potential as a complementary approach to CSIA and ESIA for assessing the biodegradation of α-HCH at contaminated field sites.
ID 14184
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=14184
Bashir, S., Fischer, A., Nijenhuis, I., Richnow, H.H. (2013):
Enantioselective carbon stable isotope fractionation of hexachlorocyclohexane during aerobic biodegradation by Sphingobium spp.
Environ. Sci. Technol. 47 (20), 11432 - 11439