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Title (Primary) Microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway
Author Martínez-Lavanchy, P.M.; Chen, Z.; Lünsmann, V.; Marin-Cevada, V.; Vilchez-Vargas, R.; Pieper, D.H.; Reiche, N.; Kappelmeyer, U.; Imparato, V.; Junca, H.; Nijenhuis, I.; Müller, J.A.; Kuschk, P.; Heipieper, H.J.;
Journal Applied and Environmental Microbiology
Year 2015
Department ISOBIO; UMB; UBT; PROTEOM;
Volume 81
Issue 18
Language englisch;
POF III (all) T41;
UFZ wide themes RU3;
Abstract In the present study, microbial toluene degradation in controlled constructed wetland model systems, Planted Fixed-bed Reactors (PFR), was queried with DNA-based methods in combination with stable isotope fractionation analysis and characterization of toluene-degrading microbial isolates. Two PFR replicates were operated with toluene as sole external carbon and electron source for two years. The bulk redox conditions in these systems were hypoxic to anoxic. The autochthonous bacterial communities, as analyzed by Illumina sequencing of 16S rRNA gene amplicons, were mainly comprised of the families Xanthomonadaceae, Comamonadeceae, and Burkholderiaceae, plus Rhodospirillaceae in one of the PFR replicates. DNA microarray analyses of the catabolic potentials for aromatic compound degradation suggested the presence of the ring monooxygenation pathway in both systems as well as the anaerobic toluene pathway in the PFR replicate with the high abundance of Rhodospirillaceae. The presence of catabolic genes encoding for the ring monooxygenation pathway was verified by qPCR analysis, utilizing the obtained toluene-degrading isolates as references. Stable isotope fractionation analysis showed low-level of carbon fractionation and only minimal hydrogen fractionation in both PFR, which matches the fractionation signatures of monooxygenation and dioxygenation. In combination with the results of the DNA-based analyses, this suggests that toluene degradation occurs predominantly via ring monooxygenation in the PFRs.
ID 16344
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16344
Martínez-Lavanchy, P.M., Chen, Z., Lünsmann, V., Marin-Cevada, V., Vilchez-Vargas, R., Pieper, D.H., Reiche, N., Kappelmeyer, U., Imparato, V., Junca, H., Nijenhuis, I., Müller, J.A., Kuschk, P., Heipieper, H.J. (2015):
Microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway
Appl. Environ. Microb. 81 (18), 6241 - 6252