Publication Details

Reference Category Journals
DOI / URL link
Creative Commons Licence creative commons licence
Title (Primary) Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene
Author Kruse, S.; Türkowsky, D.; Birkigt, J.; Matturro, B.; Franke, S.; Jehmlich, N.; von Bergen, M.; Westermann, M.; Rossetti, S.; Nijenhuis, I.; Adrian, L.; Diekert, G.; Goris, T.
Journal ISME Journal
Year 2021
Department ISOBIO; UBT; MOLSYB
Volume 15
Issue 6
Page From 1794
Page To 1809
Language englisch
Topic T7 Bioeconomy
T9 Healthy Planet
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-020-00887-6/MediaObjects/41396_2020_887_MOESM1_ESM.docx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-020-00887-6/MediaObjects/41396_2020_887_MOESM2_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41396-020-00887-6/MediaObjects/41396_2020_887_MOESM3_ESM.xlsx
UFZ wide themes ProVIS;
Abstract Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with halocarbons. To understand molecular interactions between dehalogenating bacteria, we co-cultured Sulfurospirillum multivorans, dechlorinating tetrachloroethene (PCE) to cis−1,2-dichloroethene (cDCE), and Dehalococcoides mccartyi strains BTF08 or 195, dehalogenating PCE to ethene. The co-cultures were cultivated with lactate as electron donor. In co-cultures, the bacterial cells formed aggregates and D. mccartyi established an unusual, barrel-like morphology. An extracellular matrix surrounding bacterial cells in the aggregates enhanced cell-to-cell contact. PCE was dehalogenated to ethene at least three times faster in the co-culture. The dehalogenation was carried out via PceA of S. multivorans, and PteA (a recently described PCE dehalogenase) and VcrA of D. mccartyi BTF08, as supported by protein abundance. The co-culture was not dependent on exogenous hydrogen and acetate, suggesting a syntrophic relationship in which the obligate hydrogen consumer D. mccartyi consumes hydrogen and acetate produced by S. multivorans. The cobamide cofactor of the reductive dehalogenase—mandatory for D. mccartyi—was also produced by S. multivorans. D. mccartyi strain 195 dechlorinated cDCE in the presence of norpseudo-B12 produced by S. multivorans, but D. mccartyi strain BTF08 depended on an exogenous lower cobamide ligand. This observation is important for bioremediation, since cofactor supply in the environment might be a limiting factor for PCE dehalogenation to ethene, described for D. mccartyi exclusively. The findings from this co-culture give new insights into aggregate formation and the physiology of D. mccartyi within a bacterial community.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24194
Kruse, S., Türkowsky, D., Birkigt, J., Matturro, B., Franke, S., Jehmlich, N., von Bergen, M., Westermann, M., Rossetti, S., Nijenhuis, I., Adrian, L., Diekert, G., Goris, T. (2021):
Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene
ISME J. 15 (6), 1794 - 1809