Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) Carbon stable isotope fractionation of sulfamethoxazole during biodegradation by Microbacterium sp. strain BR1 and upon direct photolysis
Author Birkigt, J.; Gilevska, T.; Ricken, B.; Richnow, H.-H.; Vione, D.; Corvini, P.F.-X.; Nijenhuis, I.; Cichocka, D.;
Journal Environmental Science & Technology
Year 2015
Department ISOBIO;
Volume 49
Issue 10
Language englisch;
POF III (all) T41;
Supplements https://pubs.acs.org/doi/suppl/10.1021/acs.est.5b00367/suppl_file/es5b00367_si_001.pdf
UFZ wide themes RU3;
Abstract Carbon isotope fractionation of sulfamethoxazole (SMX) during biodegradation by Microbacterium sp. strain BR1 (ipso-hydroxylation) and upon direct photolysis was investigated. Carbon isotope signatures (δ13C) of SMX were measured by LC-IRMS (liquid chromatography coupled to isotope ratio mass spectrometry). A new LC-IRMS method for the SMX metabolite, 3-amino-5-methylisoxazole (3A5MI), was established. Carbon isotope enrichment factors for SMX (εC) were −0.6 ± 0.1‰ for biodegradation and −2.0 ± 0.1‰ and −3.0 ± 0.2‰ for direct photolysis, at pH 7.4 and pH 5, respectively. The corresponding apparent kinetic isotope effects (AKIE) for ipso-hydroxylation were 1.006 ± 0.001; these fall in the same range as AKIE in previously studied hydroxylation reactions. The differences in SMX and 3A5MI fractionation upon biotic and abiotic degradation suggest that compound specific stable isotope analysis (CSIA) is a suitable method to distinguish SMX reaction pathways. In addition, the study revealed that the extent of isotope fractionation during SMX photolytic cleavage is pH-dependent.
ID 16158
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16158
Birkigt, J., Gilevska, T., Ricken, B., Richnow, H.-H., Vione, D., Corvini, P.F.-X., Nijenhuis, I., Cichocka, D. (2015):
Carbon stable isotope fractionation of sulfamethoxazole during biodegradation by Microbacterium sp. strain BR1 and upon direct photolysis
Environ. Sci. Technol. 49 (10), 6029 - 6036