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Title (Primary) Genome sequences of two dehalogenation specialists - Dehalococcoides mccartyi strains BTF08 and DCMB5 enriched from the highly polluted Bitterfeld region
Author Pöritz, M.; Goris, T.; Wubet, T.; Tarkka, M.T.; Buscot, F.; Nijenhuis, I.; Lechner, U.; Adrian, L.;
Journal FEMS Microbiology Letters
Year 2013
Department ISOBIO; BOOEK;
Volume 343
Issue 2
Language englisch;
POF III (all) T11;
Keywords organohalide respiration; bioremediation; reductive dehalogenase; arsenic resistance; tetrachloroethene; dioxin
UFZ wide themes RU1
Abstract The genomes of two novel Dehalococcoides mccartyi strains, DCMB5 and BTF08, enriched from the heavily organohalide-contaminated megasite around Bitterfeld (Germany), were fully sequenced and annotated. Although overall similar, the genome sequences of the two strains reveal remarkable differences in their genetic content, reflecting a specific adaptation to the contaminants at the field sites from which they were enriched. The genome of strain BTF08 encodes for 20 reductive dehalogenases, including all three of which are necessary to couple the reductive dechlorination of PCE to ethene to growth. The genes encoding trichloroethene and vinyl chloride reductive dehalogenases, tceA and vcrA, are located within mobile genetic elements, suggesting their recent horizontal acquisition. The genome of strain DCMB5 contains 23 reductive dehalogenase genes, including cbrA, which encodes a chlorobenzene reductive dehalogenase, and a gene cluster encoding arsenic resistance proteins, both corresponding to typical pollutants at its isolation site.
ID 13650
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=13650
Pöritz, M., Goris, T., Wubet, T., Tarkka, M.T., Buscot, F., Nijenhuis, I., Lechner, U., Adrian, L. (2013):
Genome sequences of two dehalogenation specialists - Dehalococcoides mccartyi strains BTF08 and DCMB5 enriched from the highly polluted Bitterfeld region
FEMS Microbiol. Lett. 343 (2), 101 - 104