Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.jhazmat.2017.02.043 |
| Title (Primary) | Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains |
| Author | Mortan, S.H.; Martín-González, L.; Vicent, T.; Caminal, G.; Nijenhuis, I.; Adrian, L.
; Marco-Urrea, E. |
| Source Titel | Journal of Hazardous Materials |
| Year | 2017 |
| Department | ISOBIO |
| Volume | 331 |
| Page From | 218 |
| Page To | 225 |
| Language | englisch |
| Keywords | Organohalide-respiring bacteria; Fed-batch reactor; Dehalococcoides; Dehalogenimonas; 1,1,2-Trichloroethane |
| UFZ wide themes | RU3; ProVIS; |
| Abstract | 1,1,2-Trichloroethane (1,1,2-TCA) is a non-flammable organic solvent and common environmental contaminant in groundwater. Organohalide-respiring bacteria are key microorganisms to remediate 1,1,2-TCA because they can gain metabolic energy during its dechlorination under anaerobic conditions. However, all current isolates produce hazardous end products such as vinyl chloride, monochloroethane or 1,2-dichloroethane that accumulate in the medium. Here, we constructed a syntrophic co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains to achieve complete detoxification of 1,1,2-TCA to ethene. In this co-culture, Dehalogenimonas transformed 1,1,2-TCA via dihaloelimination to vinyl chloride, whereas Dehalococcoides reduced vinyl chloride via hydrogenolysis to ethene. Molasses, pyruvate, and lactate supported full dechlorination of 1,1,2-TCA in serum bottle co-cultures. Scale up of the cultivation to a 5-L bioreactor operating for 76 d in fed-batch mode was successful with pyruvate as substrate. This synthetic combination of bacteria with known complementary metabolic capabilities demonstrates the potential environmental relevance of microbial cooperation to detoxify 1,1,2-TCA. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18557 |
| Mortan, S.H., Martín-González, L., Vicent, T., Caminal, G., Nijenhuis, I., Adrian, L., Marco-Urrea, E. (2017): Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains J. Hazard. Mater. 331 , 218 - 225 10.1016/j.jhazmat.2017.02.043 |
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