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Title (Primary) Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195
Author Nijenhuis, I.; Zinder, S.H.;
Journal Applied and Environmental Microbiology
Year 2005
Department ISOBIO;
Volume 71
Issue 3
Language englisch;
Abstract Dehalococcoides ethenogenes strain 195 reductively dechlorinates tetrachloroethene (PCE) and trichloroethene (TCE) to vinyl chloride and ethene using H2 as an electron donor. PCE- and TCE-reductive dehalogenase (RD) activities were mainly membrane associated, whereas only about 20% of the hydrogenase activity was membrane associated. Experiments with methyl viologen (MV) were consistent with a periplasmic location for the RDs or a component feeding electrons to them. The protonophore uncoupler tetrachlorosalicylanilide did not inhibit reductive dechlorination in cells incubated with H2 and PCE and partially restored activity in cells incubated with the ATPase inhibitor N,N′-dicyclohexylcarbodiimide. Benzyl viologen or diquat (Eo′ ≈ −360 mV) supported reductive dechlorination of PCE or TCE at rates comparable to MV (−450 mV) in cell extracts.
ID 3652
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3652
Nijenhuis, I., Zinder, S.H. (2005):
Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195
Appl. Environ. Microb. 71 (3), 1664 - 1667