Publication Details

Reference Category Journals
DOI / URL link
Title (Primary) Reductive debromination by sponge-associated anaerobic bacteria coupled to carbon isotope fractionation
Author Horna-Gray, I.; Lopez, N.A.; Nijenhuis, I.; Ahn, Y.; Richnow, H.H.; Häggblom, M.M.
Journal International Biodeterioration & Biodegradation
Year 2020
Department ISOBIO
Volume 155
Page From art. 105093
Language englisch
Keywords Anaerobic; Bromophenol; Microbial reductive dehalogenation; Carbon isotope fractionation
Abstract Marine sponges contain diverse brominated compounds as secondary metabolites and the sponge habitat appears to enrich for a population of anaerobic dehalogenating bacteria. Hence, there is interest in understanding how these natural and anthropogenic compounds are degraded in the marine environment. Compound specific isotope analysis (CSIA) is a useful tool to monitor and to quantify the degradation and fate of aquatic pollutants. The objective of this study was to evaluate whether reductive dehalogenation of brominated phenols by sponge-associated bacteria, including Desulfoluna spongiiphila, can be monitored by CSIA. Debromination of 2,6-dibromophenol to phenol by sponge-associated cultures resulted in measurable stable carbon isotope fractionation. All sponge-associated cultures showed similar isotopic enrichment factors (ε). The ε values for two independent sponge-derived dehalogenating cultures were −3.1 ± 1.5‰, and −3.0 ± 0.3‰, and that of sponge associated sediment cultures −2.0 ± 0.3‰. Thus, we demonstrate that reductive debromination of 2,6-dibromophenol resulted in measurable carbon isotope fractionation and that CSIA can be used to assess reductive debromination and to monitor and estimate in vivo dehalogenation in a sponge animal.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23781
Horna-Gray, I., Lopez, N.A., Nijenhuis, I., Ahn, Y., Richnow, H.H., Häggblom, M.M. (2020):
Reductive debromination by sponge-associated anaerobic bacteria coupled to carbon isotope fractionation
Int. Biodeterior. Biodegrad. 155 , art. 105093