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Title (Primary) Sensitive detection of anaerobic monochlorobenzene degradation using stable isotope tracers
Author Nijenhuis, I.; Stelzer, N.; Kästner, M.; Richnow, H.-H.;
Journal Environmental Science & Technology
Year 2007
Department ISOBIO; UBT;
Volume 41
Issue 11
Language englisch;
Abstract Microbial degradation of monochlorobenzene (MCB) under anaerobic conditions was investigated using a stable isotope tracer under in and ex situ conditions. In situ microcosms were incubated directly in an anoxic aquifer and carbon derived from [13C6]-MCB was found to be incorporated into the microbial biomass. In laboratory microcosms, amended with [13C6]-MCB, anaerobic mineralization of MCB was indicated by the production of 13CO2. Further, recovery of the 13C-label in the fatty acids confirmed the assimilation of MCB-derived carbon into microbial biomass. The described approach may be applied to various other organic groundwater contaminants of concern using carbon (13C) as well as other stable isotope tracers, such as nitrogen (15N), allowing direct and sensitive detection of biodegradation.
ID 2119
Persistent UFZ Identifier http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2119
Nijenhuis, I., Stelzer, N., Kästner, M., Richnow, H.-H. (2007):
Sensitive detection of anaerobic monochlorobenzene degradation using stable isotope tracers
Environ. Sci. Technol. 41 (11), 3836 - 3842