Rohit Budhraja
Phone Office +49 341 235-1412
Phone Lab +49 341 235-1549
Curriculum Vitae
| 2016-2020 | PhD student UFZ |
| 2015 - 2016 | Research Associate, Lambda Therapeutics Research Ltd., Ahmedabad, India |
| 2012 - 2015 | Research Fellow, Centre for Cellular and Molecular Biology, Hyderabad, India |
| 2010 - 2012 | Master of Science in Biotechnology, University of Rajasthan, Jaipur, India |
| 2007 - 2010 | Bachelor of Science in Biotechnology, University of Rajasthan, Jaipur, India |
Research
My research focusses on comparative metalloproteomics study of different anaerobically respiring microorganisms using ICP-MS and LC-MS. Metal ions are abundant in microbial proteins and have a structural, catalytic or electron transferring role. Approx one third of total proteins are metalloproteins that contain at least one metal ion cofactor.
We aim to investigate metalloproteins in respiring anaerobes on three levels: at the level of whole cell extracts, at the level of separated native complexes and at the level of metal cofactors with a focus on Fe-, Co-, Ni- and Mo-containing respiration complexes.
Publications
2021 (4)
- Budhraja, R., Karande, S., Ding, C., Ullrich, M.K., Wagner, S., Reemtsma, T., Adrian, L. (2021):
Characterization of membrane-bound metalloproteins in the anaerobic ammonium-oxidizing bacterium “Candidatus Kuenenia stuttgartiensis” strain CSTR1
Talanta 223 (Part 2), art. 121711 10.1016/j.talanta.2020.121711 - Chen, S.-C., Budhraja, R., Adrian, L., Calabrese, F., Stryhanyuk, H., Musat, N., Richnow, H.-H., Duan, G.-L., Zhu, Y.-G., Musat, F. (2021):
Novel clades of soil biphenyl degraders revealed by integrating isotope probing, multi-omics, and single-cell analyses
ISME J. 15 (12), 3508 - 3521 10.1038/s41396-021-01022-9 - Sharma, M., Singh, D.N., Budhraja, R., Sood, P., Rawat, C.D., Adrian, L., Richnow, H.-H., Singh, Y., Negi, R.K., Lal, R. (2021):
Comparative proteomics unravelled the hexachlorocyclohexane (HCH) isomers specific responses in an archetypical HCH degrading bacterium Sphingobium indicum B90A
Environ. Sci. Pollut. Res. 28 (30), 41380 - 41395 10.1007/s11356-021-13073-8 - Wegner, C.-E., Westermann, M., Steiniger, F., Gorniak, L., Budhraja, R., Adrian, L., Küsel, K. (2021):
Extracellular and intracellular lanthanide accumulation in the methylotrophic Beijerinckiaceae bacterium RH AL1
Appl. Environ. Microb. 87 (13), e03144-20 10.1128/AEM.03144-20
2019 (1)
- Budhraja, R., Ding, C., Walter, P., Wagner, S., Reemtsma, T., Sawers, G., Adrian, L. (2019):
The impact of species, respiration type, growth phase and genetic inventory on absolute metal content of intact bacterial cells
Metallomics 11 (5), 925 - 935 10.1039/C9MT00009G
- Budhraja, R.H., Shah, M.A., Suthar, M., Yadav, A., Shah, S.P., Kale, P., Asvadi, P., Valan Arasu, M., Al-Dhabi, N.A., Park, C.G., Kim, Y.O., Kim, H.J., Agrawal, Y.K., Krovidi, R.K., (2016) : "LC-MS/MS Validation Analysis of Trastuzumab Using dSIL Approach for Evaluating Pharmacokinetics" Molecules. 21 (11), 1464