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Title (Primary) Extracellular and intracellular lanthanide accumulation in the methylotrophic Beijerinckiaceae bacterium RH AL1
Author Wegner, C.-E.; Westermann, M.; Steiniger, F.; Gorniak, L.; Budhraja, R.; Adrian, L.; Küsel, K.;
Journal Applied and Environmental Microbiology
Year 2021
Department ISOBIO; UBT;
Volume 87
Issue 13
Language englisch;
Supplements https://journals.asm.org/doi/suppl/10.1128/AEM.03144-20/suppl_file/aem.03144-20-s0001.pdf
https://journals.asm.org/doi/suppl/10.1128/AEM.03144-20/suppl_file/aem.03144-20-s0002.xlsx
https://journals.asm.org/doi/suppl/10.1128/AEM.03144-20/suppl_file/aem.03144-20-s0003.mp4
https://journals.asm.org/doi/suppl/10.1128/AEM.03144-20/suppl_file/aem.03144-20-s0004.mp4
https://journals.asm.org/doi/suppl/10.1128/AEM.03144-20/suppl_file/aem.03144-20-s0005.mp4
Abstract Recent work with Merhylorubrum extorquens AM1 identified intracellular, cytoplasmic lanthanide storage in an organism that harnesses these metals for its metabolism. Here, we describe the extracellular and intracellular accumulation of lanthanides in the Beijerinckiaceae bacterium RH AL1, a newly isolated and recently characterized methylotroph. Using ultrathin-section transmission electron microscopy (TEM), freeze fracture TEM (FFTEM), and energy-dispersive X-ray spectroscopy, we demonstrated that strain RH AL1 accumulates lanthanides extracellularly at outer membrane vesicles (OMVs) and stores them in the periplasm. High-resolution elemental analyses of biomass samples revealed that strain RH AU can accumulate ions of different lanthanide species, with a preference for heavier lanthanides. Its methanol oxidation machinery is supposedly adapted to light lanthanides, and their selective uptake is mediated by dedicated uptake mechanisms. Based on transcriptome sequencing (RNA-seq) analysis, these presumably include the previously characterized TonB-ABC transport system encoded by the lut cluster but potentially also a type VI secretion system. A high level of constitutive expression of genes coding for lanthanide-dependent enzymes suggested that strain RH AL1 maintains a stable transcript pool to flexibly respond to changing lanthanide availability. Genes coding for lanthanide-dependent enzymes are broadly distributed taxonomically. Our results support the hypothesis that central aspects of lanthanide-dependent metabolism partially differ between the various taxa.
ID 25143
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25143
Wegner, C.-E., Westermann, M., Steiniger, F., Gorniak, L., Budhraja, R., Adrian, L., Küsel, K. (2021):
Extracellular and intracellular lanthanide accumulation in the methylotrophic Beijerinckiaceae bacterium RH AL1
Appl. Environ. Microb. 87 (13), e03144-20