Marie Eberwein

Curriculum Vitae

Research

My PhD project focuses on reducing greenhouse gas emissions by exploring more sustainable methods for chemical production. While chemicals are an integral part of our daily lives, their current production methods are neither environmentally friendly nor sustainable, relying on traditional linear processes. Microorganisms and biocatalysts, such as enzymes, offer a promising alternative to conventional chemical synthesis. A common challenge in these 'microbial cell factories' is the adequate supply of ATP, the energy currency of cells. To address this issue, we propose using the organohalide respiration complex of Dehalococcoides mccartyi strain CBDB1 as a universal energy module. By coupling this unique membrane-bound complex to an electrode, we aim to transfer electrons from the electrode through the protein complex, generating a proton motive force that drives ATP production within the cell.

Methods:

• Biochemical methods: enzymatic activity assays, Native PAGE, in-gel activity assays, protein purification
• Molecularbiological methods: Cloning, PCR, heterologous production
• Microbiological methods: Aerobe and anaerobe cultivation, microscopy
• Bioanalytical methods: nLC-MS/MS, HPLC, GC-MS, Ion- and Gaschromatography, FPLC
• Electrochemical methods: cyclic voltammetry, bioelectrochemical reactor
  

Grant

Publications