Dr. Darja Deobald
|2020-||PostDoc, Helmholtz Centre for Environmental Research (UFZ), Department of Environmental Biotechnology, Leipzig|
|2019-2020||Research assistant, University of Applied Sciences, Merseburg|
|2014-2020||PhD in Biochemistry, University of Leipzig and University of Freiburg|
|2011-2013||Master in Biochemistry, University of Leipzig|
|2008-2011||Bachelor in Biochemistry, University of Leipzig|
My research focuses on the isolation and characterization of the membrane-bound organohalide respiration complex (OHR) from Dehalococcoides mccartyi strain CBDB1 that is well known for its ability to reductively dehalogenate many chlorinated and brominated organohalides. We try to get deeper insights into the organization of the OHR complex by applying different biochemical methods like solubilization of the OHR complex with detergents, enrichment with size-exclusion chromatography or blue native-PAGE, heterologous production coupled to mass spectrometry.
Additionaly, we are interested in metabolic processes that are strongly conserved in microorganisms with ancient traits like Dehalococcoidia. By identifying, purifying and characterizing proteins involved in these processes, we can shed light in early evolution of proteins and life.
 Deobald, D.; Adrian, L.; Schöne, C.; Rother, M. and Layer, G. (2018) Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase, Sci. Rep., 8, 7404