Dr. Darja Deobald
Curriculum Vitae
| 2020- | PostDoc, Helmholtz Centre for Environmental Research (UFZ), Department of Environmental Biotechnology, Leipzig |
| 2019-2020 | Research assistant, University of Applied Sciences, Merseburg |
| 2014-2020 | PhD in Biochemistry, University of Leipzig and University of Freiburg |
| 2011-2013 | Master in Biochemistry, University of Leipzig |
| 2008-2011 | Bachelor in Biochemistry, University of Leipzig |
Research
My research focuses on the isolation and characterization of the membrane-bound organohalide respiration complex (OHR) from Dehalococcoides mccartyi strain CBDB1 that is well known for its ability to reductively dehalogenate many chlorinated and brominated organohalides. We try to get deeper insights into the organization of the OHR complex by applying different biochemical methods like solubilization of the OHR complex with detergents, enrichment with size-exclusion chromatography or blue native-PAGE, heterologous production coupled to mass spectrometry.
Additionaly, we are interested in metabolic processes that are strongly conserved in microorganisms with ancient traits like Dehalococcoidia. By identifying, purifying and characterizing proteins involved in these processes, we can shed light in early evolution of proteins and life.
Publications
2023 (3)
- Hellmold, N., Eberwein, M., Phan, M.H.T., Kümmel, S., Einsle, O., Deobald, D., Adrian, L. (2023):
Dehalococcoides mccartyi strain CBDB1 takes up protons from the cytoplasm to reductively dehalogenate organohalides indicating a new modus of proton motive force generation
Front. Microbiol. 14 , art. 1305108 10.3389/fmicb.2023.1305108 - Klaes, S., Madan, S., Deobald, D., Cooper, M., Adrian, L. (2023):
GroEL-proteotyping of bacterial communities using tandem mass spectrometry
Int. J. Mol. Sci. 24 (21), art. 15692 10.3390/ijms242115692 - Soder-Walz, J.M., Wasmund, K., Deobald, D., Vicent, T., Adrian, L., Marco-Urrea, E. (2023):
Respiratory protein interactions in Dehalobacter sp. strain 8M revealed through genomic and native proteomic analyses
Environ. Microbiol. 25 (11), 2604 - 2620 10.1111/1462-2920.16464
2022 (1)
- Deobald, D., Adrian, L. (2022):
Metallomics of the quinone-free respiration system of Dehalococcoides mccartyi indicates the presence of molybdenum, nickel, iron and cobalt in the respiratory complex
Biochim. Biophys. Acta-Bioenerg. 1863 (Suppl.), art. 148768 10.1016/j.bbabio.2022.148768
2020 (1)
- Deobald, D., Hanna, R., Shahryari, S., Layer, G., Adrian, L. (2020):
Identification and characterization of a bacterial core methionine synthase
Sci. Rep. 10 , art. 2100 10.1038/s41598-020-58873-z
2016 (1)
- Kublik, A., Deobald, D., Hartwig, S., Schiffmann, C.L., Andrades, A., von Bergen, M., Sawers, R.G., Adrian, L. (2016):
Identification of a multi-protein reductive dehalogenase complex in Dehalococcoides mccartyi strain CBDB1 suggests a protein-dependent respiratory electron transport chain obviating quinone involvement
Environ. Microbiol. 18 (9), 3044 - 3056 10.1111/1462-2920.13200
[3] Deobald, D.; Adrian, L.; Schöne, C.; Rother, M. and Layer, G. (2018) Identification of a unique Radical SAM methyltransferase required for the sp3-C-methylation of an arginine residue of methyl-coenzyme M reductase, Sci. Rep., 8, 7404