We are also deeply interested in studying metabolic processes that are highly conserved in microorganisms with ancient traits, such as Dehalococcoidia. By identifying, purifying, and characterizing the proteins involved in these "primordial" metabolic pathways, we aim to illuminate the early evolution of proteins and life itself. This research allows us to better understand how anoxic processes, along with specific proteins, contributed to the origin and evolution of life and how they continue to influence natural and artificial matter cycles today.
Ultimately, our group seeks to develop foundational concepts across various scientific fields based on insights gained from our fundamental research. We strive to translate these concepts into practical applications, bridging the gap between basic science and real-world systems.

The proposed B₁₂ methylation system offers a sustainable approach to biological methylation processes by utilizing simple C1 compounds, such as methanol or acetate, as methyl donors for methyltransferase I. These C1 compounds can be produced on an industrial scale via electrochemical CO₂ reduction, effectively functioning as a CO₂ sink. Methyltransferase I facilitates the methylation of the B₁2 shuttle protein, which in turn drives subsequent methylation reactions.
Additionally, this system reduces dependency on fossil raw materials by incorporating substrates derived from microbial processes, such as medium- and long-chain fatty acids and alcohols, as well as renewable resources and waste streams. These substrates can serve as methyl acceptors for methyltransferase II, which is responsible for transferring methyl groups to a variety of acceptor molecules, further broadening the system’s applicability and environmental benefits.

Our laboratory is equipped with a high-resolution mass spectrometer (nLC-MS/MS, Thermo Orbitrap Fusion) for the identification of proteins and metabolites, along with advanced data processing pipelines. We are pleased to offer measurement time for collaborations on environmental topics, whether through scientific partnerships or on a commercial basis.