Research Interests
I am fascinated by Nature`s designs as I believe similar principles can be used to solve numerous engineering issues. Nature`s solutions strongly motivate my research work for the application of miniaturization concepts for bioreactor development and their scale-up. I am currently working on developing a process idea based on a multistep reaction sequence using oxygenases to convert volatile, hydrophobic and toxic compounds. Additionally, I am part of a project developing efficient photobioreactors for the utilization of solar radiation and carbon dioxide for biomass generation and consequently biomaterial production.
since 2014
Scientist at the Department of Solar Materials (SoMa),
Helmholtz Centre for Environmental Research, Leipzig, Germany
2012 - 2014
Post-doctoral Researcher at the Laboratory of Chemical Biotechnology,
TU Dortmund University, Dortmund, Germany
2007 - 2012
Dissertation at the Laboratory of Chemical Biotechnology,
TU Dortmund University, Dortmund, Germany
2004 - 2007
Master of Science in Chemical Engineering (M. Sc.) with specialization in Biochemical Engineering
TU Dortmund University, Dortmund, Germany
1999 - 2003
Bachelor in Chemical Engineering (B.E)
Shivaji University (P.V.P Institute of Technology), Kolhapur, India
Index:
2019 (3)2018 (1)
2016 (2)
2015 (3)
You could use our publication index for further requests.
2019 (3)
- Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K., (2019):
Data on mixed trophies biofilm for continuous cyclohexane oxidation to cyclohexanol using Synechocystis sp. PCC 6803
Data in Brief 25 , art. 104059
full text (url) - Hoschek, A., Heuschkel, I., Schmid, A., Bühler, B., Karande, R., Bühler, K., (2019):
Mixed-species biofilms for high-cell-density application of Synechocystis sp. PCC 6803 in capillary reactors for continuous cyclohexane oxidation to cyclohexanol
Bioresour. Technol. 282 , 171 - 178
full text (url) - Hoschek, A., Toepel, J., Hochkeppel, A., Karande, R., Bühler, B., Schmid, A., (2019):
Light‐dependent and aeration‐independent gram‐scale hydroxylation of cyclohexane to cyclohexanol by CYP450 harboring Synechocystis sp. PCC 6803
J. Biotechnol.
full text (url)
2018 (1)
- Karande, R., Salamanca, D., Schmid, A., Buehler, K., (2018):
Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120
Biotechnol. Bioeng. 115 (2), 312 - 320
final draft (pdf)
2016 (2)
- Halan, B., Karande, R., Buehler, K., Schmid, A., (2016):
Catalytic Pseudomonas taiwanensis VLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor
J. Flow Chem. 6 (1), 39 - 42
full text (url) - Karande, R., Schmid, A., Buehler, K., (2016):
Applications of multiphasic microreactors for biocatalytic reactions
Org. Process Res. Dev. 20 (2), 361 - 370
full text (url)
2015 (3)
- Karande, R., Debor, L., Salamanca, D., Bogdahn, F., Engesser, K.-H., Buehler, K., Schmid, A., (2015):
Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms
Biotechnol. Bioeng. 113 (1), 52 - 61
full text (url) - Salamanca, D., Karande, R., Schmid, A., Dobslaw, D., (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897
full text (url) - Willrodt, C., Karande, R., Schmid, A., Julsing, M.K., (2015):
Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants
Curr. Opin. Biotechnol. 35 , 52 - 62
full text (url)
2014
- Karande R.*, Halan B.*, Schmid A. and Bühler K. (2014)
Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.
Biotechnology and Bioengineering, 111(9):1831-1840, DOI-Link
* These authors contributed equally to this work.
2011
- Ruinatscha R., Karande R., Bühler K. and Schmid A. (2011)
Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using Sepabead EC-EA and EC-Q1A anion-exchange carriers.
Molecules, 16(7):5975-5988, DOI-Link
- Karande R., Schmid A. and Bühler K. (2011)
Miniaturizing Biocatalysis: Enzyme catalyzed reactions in an aqueous/organic segmented flow capillary microreactor.
Advanced Synthesis and Catalysis, 353(13):2511-2521, DOI-Link
2010
- Karande R., Schmid A. and Bühler K. (2010)
Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity.
Langmuir, 26(11):9152-9159, DOI-Link
Books
2017
- Karande R., Schmid A. and Bühler K. (2017)
Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
Springer, Berlin, Germany
2014
- Karande R. (2014)
Development and Application of Microreactors for Biocatalytic ReactionsShaker Verlag GmbH, Herzogenrath, Germany, ISBN: 978-3-8440-2833-1
Patent Applications
2018
- Karande R., Hoschek A., Heuschkel I., Bühler K., Schmid A.
"A composition of photoautotrophic microorganisms and chemoheterotrophic microorganisms in a biofilm"
PCT/EP2018/074746, filed
- Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A.
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
WO/2018/046104
2016
- Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A. (2016)
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
WO/2018/046104
2011
- Schmid A., Karande R. and Bühler K. (2011)
Segmented Flow Biofilm Reactor.
WO/2012/152337
Oral Presentations
2015
- Karande R., Schmid A. and Buehler K. (2015)
Application of multiphasic microreactors for bioprocess intensification
IMTB, 10-13 May 2015, Opatja, Croatia
2013
- Karande R., Schmid A. and Buehler K. (2013)
Segmented flow biofilm membrane microreactor-a new reactor concept
IMTB, 05-08 May 2013, Cavtat, Croatia
2012
- Karande R., Bühler K. and Schmid A. (2012)
Segmented flow microreactors.
ESBES, 23. - 26. September 2012, Istanbul, Turkey
2011
- Karande R., Bühler K. and Schmid A. (2011)
Segmented flow capillary microreactors: a tool for bio-process intensification.
DECHEMA - Bioverfahrenstechnik an Grenzflächen, 30. - 1.June 2011, Potsdam, Germany
2010
- Karande R., Bühler K. and Schmid A. (2010)
Segment flow capillary microreactors as alternatives to two phase enzymatic batch reactors.
ESBES, 08. - 10. September 2010, Bologna, Italy
2009
- Karande R., Bühler K. and Schmid A. (2009)
Application of segmented flow microsystems to enzymatic catalysis.
Kolloquium der Fakultät BCI, 11.Nov. 2009, Dortmund, Germany
Poster
2015
- Halan B., Karande R., Schmid A. and Bühler K. (2015)
Scale-up and scale-down of truly continous biofilm based bioprocesses.
DECHEMA Himmelfahrtstagung, 11. - 13. May 2015, Hamburg-Bergedorf, Germany - Halan B., Karande R., Schmid A. and Bühler K. (2015)
Industrial biotechnology for sustainable and energy efficient bioprocesses: miniaturized biofilm based
continuous fine chemical synthesis.
UFZ Energy Days, 24. - 25. February 2015, Leipzig, Germany
2011
- Karande R., Bühler K. and Schmid A. (2011)
Applications of segmented flow microreactors for biocatalysis.
Biotrends, 8. - 9. December 2011, Dortmund, Germany
2010
- Karande R., Bühler K. and Schmid A. (2010)
Asymmetric enzymatic hydrogenation in an aqueous/organic flow capillary microreaktor.
Bioprozessorientiertes Anlagendesign, 10. - 12. Mai 2010, Nürnberg, Germany
2009
- Karande R., Bühler K. and Schmid A. (2009)
Multiphase slug flow microreactor for enzyme based redox biocatalysis.
Dechema, Jahrestagung der Biotechnologen, 8. - 10. September 2009, Mannheim, Germany
2008
- Karande R., Bühler K. and Schmid A. (2008)
Asymmetric Enzymatic Hydrogenation in Capillary Microreactors.
MSB, 9. - 13. March 2008, Berlin, Germany