Dr.-Ing. Rohan Karande
Research Interests
Redox and multistep biocatalysis using microbial cells, catalytic biofilms, reactor engineering, continuous bioprocess, microreactors, photobiotechnology
since 2021
Junior Research Group Leader
“Bioactive systems for controlled evolutionary materials”
The interfaculty Centre for Bioactive Matter (bACTmatter)
University of Leipzig, Germany
since 2014
Scientist at the Department of Solar Materials (SoMa),
Helmholtz Centre for Environmental Research, Leipzig, Germany
2012 - 2014
Post-doctoral Researcher at the Laboratory of Chemical Biotechnology,
TU Dortmund University, Dortmund, Germany
2007 - 2012
Dissertation at the Laboratory of Chemical Biotechnology,
TU Dortmund University, Dortmund, Germany
2004 - 2007
Master of Science in Chemical Engineering (M. Sc.) with specialization in Biochemical Engineering
TU Dortmund University, Dortmund, Germany
1999 - 2003
Bachelor in Chemical Engineering (B.E)
Shivaji University (P.V.P Institute of Technology), Kolhapur, India
Index:
You could use our publication index for further requests.
2022 (2)
- Bretschneider, L., Heuschkel, I., Bühler, K., Karande, R., Bühler, B. (2022):
Rational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB120
Metab. Eng. 70 , 206 - 217 10.1016/j.ymben.2022.01.014 - Siebert, N.A., Franz, A., Karande, R. (2022):
Phototrophe Biofilme für die kontinuierliche Produktion von Chemikalien [Phototrophic biofilms for the continuous production of chemicals]
Biospektrum 28 (2), 212 - 214 10.1007/s12268-022-1723-8
2021 (6)
- Bretschneider, L., Heuschkel, I., Ahmed, A., Bühler, K., Karande, R., Bühler, B. (2021):
Characterization of different biocatalyst formats for BVMO‐catalyzed cyclohexanone oxidation
Biotechnol. Bioeng. 118 (7), 2719 - 2733 10.1002/bit.27791 - Bretschneider, L., Heuschkel, I., Wegner, M., Lindmeyer, M., Bühler, K., Karande, R., Bühler, B. (2021):
Conversion of cyclohexane to 6-hydroxyhexanoic acid using recombinant Pseudomonas taiwanensis in a stirred-tank bioreactor
Front. Catal. 1 , art. 683248 10.3389/fctls.2021.683248 - Bretschneider, L., Wegner, M., Bühler, K., Bühler, B., Karande, R. (2021):
One-pot synthesis of 6-aminohexanoic acid from cyclohexane using mixed-species cultures
Microb. Biotechnol. 14 (3), 1011 - 1025 10.1111/1751-7915.13744 - David, C., Karande, R., Bühler, K. (2021):
Cyanobacterial biofilms in natural and synthetic environments
In: Nielsen, J., Lee, S., Stephanopoulos, G., Hudson, P. (eds.)
Cyanobacteria biotechnology
Wiley, Weinheim, p. 477 - 503 10.1002/9783527824908.ch15 - Heuschkel, I., Hanisch, S., Volke, D.C., Löfgren, E., Hoschek, A., Nikel, P.I., Karande, R., Bühler, K. (2021):
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors
Eng. Life Sci. 21 (3-4), 258 - 269 10.1002/elsc.202000072 - Salamanca, D., Bühler, K., Engesser, K.-H., Schmid, A., Karande, R. (2021):
Whole-cell biocatalysis using the Acidovorax sp. CHX100 Δ6HX for the production of ω-hydroxycarboxylic acids from cycloalkanes
New Biotech. 60 , 200 - 206 10.1016/j.nbt.2020.10.009
2020 (4)
- David, C., Heuschkel, I., Bühler, K., Karande, R. (2020):
Cultivation of productive biofilms in flow reactors and their characterization by CLSM
In: Guisan, J.M., Bolivar, J.M., López-Gallego, F., Rocha-Martín, J. (eds.)
Immobilization of Enzymes and Cells
Methods in Molecular Biology 2100
Springer Nature, p. 437 - 452 10.1007/978-1-0716-0215-7_30 - Heuschkel, I., Dagini, R., Karande, R., Bühler, K. (2020):
The impact of glass material on growth and biocatalytic performance of mixed-species biofilms in capillary reactors for continuous cyclohexanol production
Front. Bioeng. Biotechnol. 8 , art. 588729 10.3389/fbioe.2020.588729 - Schäfer, L., Bühler, K., Karande, R., Bühler, B. (2020):
Rational engineering of a multi-step biocatalytic cascade for the conversion of cyclohexane to polycaprolactone monomers in Pseudomonas taiwanensis
Biotechnol. J. 15 (11), art. 2000091 10.1002/biot.202000091 - Schäfer, L., Karande, R., Bühler, B. (2020):
Maximizing biocatalytic cyclohexane hydroxylation by modulating cytochrome P450 monooxygenase expression in P. taiwanensis VLB120
Front. Bioeng. Biotechnol. 8 , art. 140 10.3389/fbioe.2020.00140
2019 (4)
- Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Data on mixed trophies biofilm for continuous cyclohexane oxidation to cyclohexanol using Synechocystis sp. PCC 6803
Data Brief 25 , art. 104059 10.1016/j.dib.2019.104059 - Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-trophies biofilm cultivation in capillary reactors
MethodsX 6 , 1822 - 1831 10.1016/j.mex.2019.07.021 - Hoschek, A., Heuschkel, I., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-species biofilms for high-cell-density application of Synechocystis sp. PCC 6803 in capillary reactors for continuous cyclohexane oxidation to cyclohexanol
Bioresour. Technol. 282 , 171 - 178 10.1016/j.biortech.2019.02.093 - Hoschek, A., Toepel, J., Hochkeppel, A., Karande, R., Bühler, B., Schmid, A. (2019):
Light‐dependent and aeration‐independent gram‐scale hydroxylation of cyclohexane to cyclohexanol by CYP450 harboring Synechocystis sp. PCC 6803
Biotechnol. J. 14 (8), art. 1800724 10.1002/biot.201800724
2018 (1)
- Karande, R., Salamanca, D., Schmid, A., Buehler, K. (2018):
Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120
Biotechnol. Bioeng. 115 (2), 312 - 320 10.1002/bit.26469
2016 (3)
- Halan, B., Karande, R., Buehler, K., Schmid, A. (2016):
Catalytic Pseudomonas taiwanensis VLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor
J. Flow Chem. 6 (1), 39 - 42 10.1556/1846.2015.00037 - Karande, R., Debor, L., Salamanca, D., Bogdahn, F., Engesser, K.-H., Buehler, K., Schmid, A. (2016):
Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms
Biotechnol. Bioeng. 113 (1), 52 - 61 10.1002/bit.25696 - Karande, R., Schmid, A., Buehler, K. (2016):
Applications of multiphasic microreactors for biocatalytic reactions
Org. Process Res. Dev. 20 (2), 361 - 370 10.1021/acs.oprd.5b00352
2015 (2)
- Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897 10.1007/s00253-015-6599-9 - Willrodt, C., Karande, R., Schmid, A., Julsing, M.K. (2015):
Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants
Curr. Opin. Biotechnol. 35 , 52 - 62 10.1016/j.copbio.2015.03.010
2014
- Karande R.*, Halan B.*, Schmid A. and Bühler K. (2014)
Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.
Biotechnology and Bioengineering, 111(9):1831-1840, DOI-Link
* These authors contributed equally to this work.
2011
- Ruinatscha R., Karande R., Bühler K. and Schmid A. (2011)
Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using Sepabead EC-EA and EC-Q1A anion-exchange carriers.
Molecules, 16(7):5975-5988, DOI-Link
- Karande R., Schmid A. and Bühler K. (2011)
Miniaturizing Biocatalysis: Enzyme catalyzed reactions in an aqueous/organic segmented flow capillary microreactor.
Advanced Synthesis and Catalysis, 353(13):2511-2521, DOI-Link
2010
- Karande R., Schmid A. and Bühler K. (2010)
Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity.
Langmuir, 26(11):9152-9159, DOI-Link
Books
2017
- Karande R., Schmid A. and Bühler K. (2017)
Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
Springer, Berlin, Germany
2014
- Karande R. (2014)
Development and Application of Microreactors for Biocatalytic ReactionsShaker Verlag GmbH, Herzogenrath, Germany, ISBN: 978-3-8440-2833-1
Patent Applications
2018
- Karande R., Hoschek A., Heuschkel I., Bühler K., Schmid A.
"A composition of photoautotrophic microorganisms and chemoheterotrophic microorganisms in a biofilm"
PCT/EP2018/074746, filed
- Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A.
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
WO/2018/046104
2016
- Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A. (2016)
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
WO/2018/046104
2011
- Schmid A., Karande R. and Bühler K. (2011)
Segmented Flow Biofilm Reactor.
WO/2012/152337
Oral Presentations
2015
- Karande R., Schmid A. and Buehler K. (2015)
Application of multiphasic microreactors for bioprocess intensification
IMTB, 10-13 May 2015, Opatja, Croatia
2013
- Karande R., Schmid A. and Buehler K. (2013)
Segmented flow biofilm membrane microreactor-a new reactor concept
IMTB, 05-08 May 2013, Cavtat, Croatia
2012
- Karande R., Bühler K. and Schmid A. (2012)
Segmented flow microreactors.
ESBES, 23. - 26. September 2012, Istanbul, Turkey
2011
- Karande R., Bühler K. and Schmid A. (2011)
Segmented flow capillary microreactors: a tool for bio-process intensification.
DECHEMA - Bioverfahrenstechnik an Grenzflächen, 30. - 1.June 2011, Potsdam, Germany
2010
- Karande R., Bühler K. and Schmid A. (2010)
Segment flow capillary microreactors as alternatives to two phase enzymatic batch reactors.
ESBES, 08. - 10. September 2010, Bologna, Italy
2009
- Karande R., Bühler K. and Schmid A. (2009)
Application of segmented flow microsystems to enzymatic catalysis.
Kolloquium der Fakultät BCI, 11.Nov. 2009, Dortmund, Germany
Poster
2015
- Halan B., Karande R., Schmid A. and Bühler K. (2015)
Scale-up and scale-down of truly continous biofilm based bioprocesses.
DECHEMA Himmelfahrtstagung, 11. - 13. May 2015, Hamburg-Bergedorf, Germany - Halan B., Karande R., Schmid A. and Bühler K. (2015)
Industrial biotechnology for sustainable and energy efficient bioprocesses: miniaturized biofilm based
continuous fine chemical synthesis.
UFZ Energy Days, 24. - 25. February 2015, Leipzig, Germany
2011
- Karande R., Bühler K. and Schmid A. (2011)
Applications of segmented flow microreactors for biocatalysis.
Biotrends, 8. - 9. December 2011, Dortmund, Germany
2010
- Karande R., Bühler K. and Schmid A. (2010)
Asymmetric enzymatic hydrogenation in an aqueous/organic flow capillary microreaktor.
Bioprozessorientiertes Anlagendesign, 10. - 12. Mai 2010, Nürnberg, Germany
2009
- Karande R., Bühler K. and Schmid A. (2009)
Multiphase slug flow microreactor for enzyme based redox biocatalysis.
Dechema, Jahrestagung der Biotechnologen, 8. - 10. September 2009, Mannheim, Germany
2008
- Karande R., Bühler K. and Schmid A. (2008)
Asymmetric Enzymatic Hydrogenation in Capillary Microreactors.
MSB, 9. - 13. March 2008, Berlin, Germany