Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/elsc.202000072 |
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| Title (Primary) | Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
| Author | Heuschkel, I.; Hanisch, S.; Volke, D.C.; Löfgren, E.; Hoschek, A.; Nikel, P.I.; Karande, R.; Bühler, K. |
| Source Titel | Engineering in Life Sciences |
| Year | 2021 |
| Department | SOMA |
| Volume | 21 |
| Issue | 3-4 |
| Page From | 258 |
| Page To | 269 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Felsc.202000072&file=elsc1367-sup-0001-SuppMat.pdf |
| Keywords | Baeyer‐Villiger oxidation; biofilm reactors; biotransformation; continuous bioprocess; cyclohexanone monooxygenase |
| Abstract | In this study, the biocatalytic performance of a Baeyer‐Villiger monooxygenase (BVMO) catalyzing the reaction of cyclohexanone to ε‐caprolactone was investigated in Pseudomonas biofilms. Biofilm growth and development of two Pseudomonas taiwanensis VLB120 variants, Ps_BVMO and Ps_BVMO_DGC, were evaluated in drip flow reactors (DFRs) and rotating bed reactors (RBRs). Engineering a hyperactive diguanylate cyclase (DGC) from Caulobacter crescentus into Ps_BVMO resulted in faster biofilm growth compared to the control Ps_BVMO strain in the DFRs. The maximum product formation rates of 92 and 87 g m–2 d–1 were observed for mature Ps_BVMO and Ps_ BVMO_DGC biofilms, respectively. The application of the engineered variants in the RBR was challenged by low biofilm surface coverage (50–60%) of rotating bed cassettes, side‐products formation, oxygen limitation, and a severe drop in production rates with time. By implementing an active oxygen supply mode and a twin capillary spray feed, the biofilm surface coverage was maximized to 70–80%. BVMO activity was severely inhibited by cyclohexanol formation, resulting in a decrease in product formation rates. By controlling the cyclohexanone feed concentration at 4 mM, a stable product formation rate of 14 g m–2 d–1 and a substrate conversion of 60% was achieved in the RBR. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24180 |
| Heuschkel, I., Hanisch, S., Volke, D.C., Löfgren, E., Hoschek, A., Nikel, P.I., Karande, R., Bühler, K. (2021): Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors Eng. Life Sci. 21 (3-4), 258 - 269 10.1002/elsc.202000072 |
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