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Title (Primary) Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors
Author Heuschkel, I.; Hanisch, S.; Volke, D.C.; Löfgren, E.; Hoschek, A.; Nikel, P.I.; Karande, R.; Bühler, K.
Journal Engineering in Life Sciences
Year 2021
Department SOMA
Volume 21
Issue 3-4
Page From 258
Page To 269
Language englisch
Topic T7 Bioeconomy
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Felsc.202000072&file=elsc1367-sup-0001-SuppMat.pdf
Keywords Baeyer‐Villiger oxidation; biofilm reactors; biotransformation; continuous bioprocess; cyclohexanone monooxygenase
Abstract In this study, the biocatalytic performance of a Baeyer‐Villiger monooxygenase (BVMO) catalyzing the reaction of cyclohexanone to ε‐caprolactone was investigated in Pseudomonas biofilms. Biofilm growth and development of two Pseudomonas taiwanensis VLB120 variants, Ps_BVMO and Ps_BVMO_DGC, were evaluated in drip flow reactors (DFRs) and rotating bed reactors (RBRs). Engineering a hyperactive diguanylate cyclase (DGC) from Caulobacter crescentus into Ps_BVMO resulted in faster biofilm growth compared to the control Ps_BVMO strain in the DFRs. The maximum product formation rates of 92 and 87 g m–2 d–1 were observed for mature Ps_BVMO and Ps_ BVMO_DGC biofilms, respectively. The application of the engineered variants in the RBR was challenged by low biofilm surface coverage (50–60%) of rotating bed cassettes, side‐products formation, oxygen limitation, and a severe drop in production rates with time. By implementing an active oxygen supply mode and a twin capillary spray feed, the biofilm surface coverage was maximized to 70–80%. BVMO activity was severely inhibited by cyclohexanol formation, resulting in a decrease in product formation rates. By controlling the cyclohexanone feed concentration at 4 mM, a stable product formation rate of 14 g m–2 d–1 and a substrate conversion of 60% was achieved in the RBR.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24180
Heuschkel, I., Hanisch, S., Volke, D.C., Löfgren, E., Hoschek, A., Nikel, P.I., Karande, R., Bühler, K. (2021):
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors
Eng. Life Sci. 21 (3-4), 258 - 269