Publication Details

Category Text Publication
Reference Category Book chapters
DOI 10.1007/978-1-0716-0215-7_30
Title (Primary) Cultivation of productive biofilms in flow reactors and their characterization by CLSM
Title (Secondary) Immobilization of Enzymes and Cells
Author David, C.; Heuschkel, I.; Bühler, K.; Karande, R.
Publisher Guisan, J.M.; Bolivar, J.M.; López-Gallego, F.; Rocha-Martín, J.
Source Titel Methods in Molecular Biology
Year 2020
Department SOMA
Volume 2100
Page From 437
Page To 452
Language englisch
Keywords Biofilm; CLSM; Biocatalysis; Segmented flow; Pseudomonas
Abstract Biofilms, a natural form of immobilized whole cells, are currently being investigated as a robust biocatalyst for the production of chemicals. Fluidic conditions and reactor geometry severely influence biofilm growth, development, and reaction performance. However, there is a missing link between the in situ characterization of biofilms on microscale setups and macroscale reactors because of the difference in reactor geometry and fluidic conditions. In this protocol, we describe the assembly and operation of flow cell and flow reactor setups with identical system geometry and segmented flow conditions to link biofilm characterization to reactor performance. The flow cell setup enables the in situ characterization of biofilm growth, structural development, and cell viability by utilizing confocal laser scanning microscopy (CLSM). Whereas, the laboratory scale flow reactor allows the determination of overall biofilm dry mass, catalytic activity, and final product titer during biocatalysis. Finally, CLSM image acquisition and the following data analysis are briefly described.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22866
David, C., Heuschkel, I., Bühler, K., Karande, R. (2020):
Cultivation of productive biofilms in flow reactors and their characterization by CLSM
In: Guisan, J.M., Bolivar, J.M., López-Gallego, F., Rocha-Martín, J. (eds.)
Immobilization of Enzymes and Cells
Methods in Molecular Biology 2100
Springer Nature, p. 437 - 452