The cytometric fingerprint is converted to an image in which the subcommunities are represented by black blots (optional grey level blots) on a white background. The dissimilarity of the cytometric fingerprints is calculated based on image comparison.
An instruction manual for performing the Dalmatian plot procedure including ready-to-use macros for ImageJ and R can be downloaded
Bombach P, Hübschmann T, Fetzer I, Kleinsteuber S, Geyer R, Harms H, Müller S. Resolution of natural microbial community dynamics by community fingerprinting, flow cytometry and trend interpretation analysis. In: Advances in Biochemical Engineering and Biotechnology: ‘High Resolution Microbial Single Cell Analytics’ Guest Ed: Susann Müller, Thomas Bley; Springer Berlin (2011) 124; 151-181.