Dr. Guillaume Lentendu
Contact / Address
Dr. Guillaume Lentendu
PostDoc
Department of Soil Ecology
Helmholtz Centre for Environmental Research - UFZ
Theodor-Lieser-Str. 4 | 06120 Halle/Saale
Germany
Tel.:
Fax:
Email:
Web:
+49 345 558 5207
+49 345 558 5449
guillaume.lentendu@ufz.de
ResearchGate
Research interest
- Microbial cross-kingdom interactions in the rhizosphere
- Bioinformatic development of amplicon pipeline for microbial diversity meta-barcoding
- Fertilisation as driver of soil microbial diversity assemblages
- Intra-specific plant genome variations effects on soil microbial communities
- Method development for flow cytometry cell sorting of soil uncultured eukaryotic microbes
Funding
German Federation for Biological Data
(
GFBio
)
PhD thesis
My thesis intended to develop methods to facilitate the study of soil eukaryotic microbes as well as to enhance the understanding of their ecological role in the soil food web and nutrient cycles of agroecosystems. A cell extraction method was first developed and evaluated for its quantitative and qualitative ability to recover eukaryotic microbial cells in a particle free suspension. Cells were sorted based on their natural fluorescence by mean of flow cytometry, thus allowing the separation of photo-autotrophic and heterotrophic organisms. Deep molecular characterisation based on a multiple barcoding approach was also applied to assess the long term effect of crop fertilisation on the diversity and community composition of different groups of soil eukaryotic microbes. The comparison between group specific barcodes and a universal eukaryotic barcode further aimed to challenge the accuracy of the latter approach to measure soil microbial eukaryotic diversity. At a final stage, the diversity and community composition of micro-algae and fungi in the sorted cell fractions were compared to their originated soil. Micro-algal and fungal biomarkers associated with the different organic and mineral long term fertilisation treatments were uncovered and their co-occurrence patterns in relation to fertilisation were assessed.
Publications
2020 (1)
- Theodorou, P., Radzevičiūtė, R., Lentendu, G., Kahnt, B., Husemann, M., Bleidorn, C., Settele, J., Schweiger, O., Grosse, I., Wubet, T., Murray, T.E., Paxton, R.J. (2020):
Urban areas as hotspots for bees and pollination but not a panacea for all insects
Nat. Commun. 11 , art. 576 10.1038/s41467-020-14496-6
2019 (1)
- Banos, S., Lentendu, G., Kopf, A., Wubet, T., Glöckner, F.O., Reich, M. (2019):
Correction to: A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms (vol 18, 190, 2018)
BMC Microbiol. 19 , art. 249 10.1186/s12866-019-1628-y
2018 (5)
- Banos, S., Lentendu, G., Kopf, A., Wubet, T., Glöckner, F.O., Reich, M. (2018):
A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms
BMC Microbiol. 18 , art. 190 10.1186/s12866-018-1331-4 - Purahong, W., Wubet, T., Kahl, T., Arnstadt, T., Hoppe, B., Lentendu, G., Baber, K., Rose, T., Kellner, H., Hofrichter, M., Bauhus, J., Krüger, D., Buscot, F. (2018):
Increasing N deposition impacts neither diversity nor functions of deadwood‐inhabiting fungal communities, but adaptation and functional redundancy ensure ecosystem function
Environ. Microbiol. 20 (5), 1693 - 1710 10.1111/1462-2920.14081 - Purahong, W., Wubet, T., Lentendu, G., Hoppe, B., Jariyavidyanont, K., Arnstadt, T., Baber, K., Otto, P., Kellner, H., Hofrichter, M., Bauhus, J., Weisser, W.W., Krüger, D., Schulze, E.-D., Kahl, T., Buscot, F. (2018):
Determinants of deadwood-inhabiting fungal communities in temperate forests: Molecular evidence from a large scale deadwood decomposition experiment
Front. Microbiol. 9 , art. 2120 10.3389/fmicb.2018.02120 - Schöps, R., Goldmann, K., Herz, K., Lentendu, G., Schöning, I., Bruelheide, H., Wubet, T., Buscot, F. (2018):
Land-use intensity rather than plant functional identity shapes bacterial and fungal rhizosphere communities
Front. Microbiol. 9 , art. 2711 10.3389/fmicb.2018.02711 - Weißbecker, C., Wubet, T., Lentendu, G., Kühn, P., Scholten, T., Bruelheide, H., Buscot, F. (2018):
Experimental evidence of functional group-dependent effects of tree diversity on soil fungi in subtropical forests
Front. Microbiol. 9 , art. 2312 10.3389/fmicb.2018.02312
2017 (2)
- Morawe, M., Hoeke, H., Wissenbach, D.K., Lentendu, G., Wubet, T., Kröber, E., Kolb, S. (2017):
Acidotolerant Bacteria and fungi as a sink of methanol-derived carbon in a deciduous forest soil
Front. Microbiol. 8 , 1361 10.3389/fmicb.2017.01361 - Purahong, W., Pietsch, K.A., Lentendu, G., Schöps, R., Bruelheide, H., Wirth, C., Buscot, F., Wubet, T. (2017):
Characterization of unexplored deadwood mycobiome in highly diverse subtropical forests using culture-independent molecular technique
Front. Microbiol. 8 , art. 574 10.3389/fmicb.2017.00574
2016 (2)
- Francioli, D., Schulz, E., Lentendu, G., Wubet, T., Buscot, F., Reitz, T. (2016):
Mineral vs. organic amendments: microbial community structure, activity and abundance of agriculturally relevant microbes are driven by long-term fertilization strategies
Front. Microbiol. 7 , art. 1446 10.3389/fmicb.2016.01446 - Purahong, W., Wubet, T., Lentendu, G., Schloter, G., Pecyna, M.J., Kapturska, D., Hofrichter, M., Krüger, D., Buscot, F. (2016):
Life in leaf litter: novel insights into community dynamics of bacteria and fungi during litter decomposition
Mol. Ecol. 25 (16), 4059 - 4074 10.1111/mec.13739
2015 (2)
- Purahong, W., Stempfhuber, B., Lentendu, G., Francioli, D., Reitz, T., Buscot, F., Schloter, M., Krüger, D. (2015):
Influence of commonly used primer systems on automated ribosomal intergenic spacer analysis of bacterial communities in environmental samples
PLOS One 10 (3), e0118967 10.1371/journal.pone.0118967 - Ramirez, K.S., Döring, M., Eisenhauer, N., Gardi, C., Ladau, J., Leff, J.W., Lentendu, G., Lindo, Z., Rillig, M.C., Russell, D., Scheu, S., St. John, M.G., de Vries, F., Wubet, T., van der Putten, W.H., Wall, D.H. (2015):
Toward a global platform for linking soil biodiversity data
Front. Ecol. Evol. 3 , art. 91 10.3389/fevo.2015.00091
2014 (1)
- Lentendu, G., Wubet, T., Chatzinotas, A., Wilhelm, C., Buscot, F., Schlegel, M. (2014):
Effects of long term differential fertilization on eukaryotic microbial communities in an arable soil: a multiple barcoding approach
Mol. Ecol. 23 (13), 3341 - 3355 10.1111/mec.12819
2013 (1)
- Lentendu, G., Hübschmann, T., Müller, S., Dunker, S., Buscot, F., Wilhelm, C. (2013):
Recovery of soil unicellular eukaryotes: an efficiency and activity analysis on the single cell level
J. Microbiol. Methods 95 (3), 463 - 469 10.1016/j.mimet.2013.05.006
2011 (1)
- Lentendu, G., Zinger, L., Manel, S., Coissac, E., Choler, P., Geremia, R.A., Melodelima, C. (2011):
Assessment of soil fungal diversity in different alpine tundra habitats by means of pyrosequencing
Fungal Divers. 49 (1), 113 - 123 10.1007/s13225-011-0101-5
Review activities
Journals: Systematics and Biodiversity, Cytometry Part A, Molecular Ecology, Molecular Ecology Ressources, Scientific Reports
Expertises: microbial diversity, NGS, meta-barcoding, soil biodiversity
Invited talks
Lentendu G. (2015) High-throughput single cell and molecular methods to analyse soil eukaryotic microbes and their long term changes in response to soil fertilisation, Ecological Symposium, University of Köln, Feb. 14
Lentendu G. (2014) Metagenomics and amplicon sequencing for species and phylogenetic diversity assessment of complex communities, iSEQ workshop, iDiv Leipzig, Nov. 13-14
Lentendu G. (2013) Effect of long term differential fertilization on the soil microbial eukaryotic community, Laboratoire Ampère, Ecole Centrale de Lyon, Oct. 14
Lentendu G. (2013) Effect of long term differential fertilization on the soil microbial eukaryotic community, Molecular Systematics Group, University of Geneva, Oct 15
Congress contributions
Oral communications
Effects of long term differential fertilization on microbial eukaryotic community of an arable soil: a multiple barcoding approach, 33rd annual meeting of the German Society for Protozoology, Essen – Germany, Feb. 2014
Soil eukaryotic unicellular microorganisms facing crop fertilization: a metagenomic approach, Annual Conference of the Association for General and Applied Microbiology (VAAM), Bremen - Germany, Mar. 2013
Soil eukaryotic unicellular microorganisms facing crop fertilization, BioSyst.EU 2013 Global systematics!, Vienna - Austria, Feb. 2013
High-throughput analysis of soil eukaryotic unicellular microorganisms: from cytometry to pyrosequencing, 5th International Conference on Analysis of Microbial Cells at the Single Cell Level, Marseille - France, Nov. 2011Posters
Lentendu G., Schmidt J., Schultz E., Buscot F., Wubet T. (2015) Alfalfa root symbionts under soil nutrient pressure: cooperation or competition?, 13th Symposium on Bacterial Genetics and Ecology, Milan, Italy, Jun. 14-18
Lentendu G., Schmidt J., Schultz E., Buscot F., Wubet T. (2014) How do crop fertilization affect the soil microbiome on a long-term basis?, The First Global Soil Biodiversity Conference, Dijon, France, Dec. 2-5
Lentendu G., Chatzinotas A., Müller S., Wubet T., Buscot F., Wilhelm C., Schlegel M., Effect of long term differential fertilization on the soil microbial eukaryotic community, 2nd Thünen Symposium on Soil Metagenomics, Braunschweig - Germany, December 2013
Lentendu G., Wubet T., Hübschmann T., Müller S., Wilhelm C., Schlegel M., Buscot F., Soil unicellular eukaryotic community analyzed at the single cell level: coupling flow cytometry and metagenomics, the 5th Congress of European Microbiologist, Leipzig - Germany, July 2013
Lentendu G., Müller S., Hübschmann T., Chatzinotas A., Wubet T., Buscot F., Schlegel M., Wilhelm C., High-throughput analysis of soil eukaryotic unicellular microorganisms: from cytometry to pyrosequencing, Workshop of the GfÖ Soil Ecology Group, Berlin - Germany, Oct. 2011
Teaching
Submission of raw amplicon sequences and their associated metadata to Short Read Archives databases, UFZ Soil Ecology bioinformatic courses, Halle/Salle, Germany, Jan. 2015
Introduction to unix bash language, OBITools and virtual In silico PCR, UFZ Soil Ecology bioninformatic courses, Halle/Salle - Germany, June 2011
Education
since 2015
PostDoc at the Department of Soil Ecology – UFZ Halle
Mar. 2010 - Dec. 2014
PhD Student at the Department of Soil Ecology – UFZ, the Plant Physiology Laboratory – University of Leipzig, and the Molecular Evolution & Animal Systematic Laboratory – University of Leipzig
Jan. 2009 - Feb. 2010
MSc. thesis and assistantship in microbial ecology: “Use of new molecular and statistical tools to improve estimation of soil microbial diversity”
June 2008
Field internship : determination of birth pick of izard (Rupicapra pyrenaica) in the RNCFS of Orlu (French Pyrenees)
Feb. 2008 - Apr. 2008
Research internship: « Inferring historical evolution of male macaque populations with SNPs », in the laboratory Evolution et Diversité Biologique (EDB) in Toulouse
Sep. 2007 - Dec. 2008
Master of science courses in ecology, evolution and biodiversity at the Toulouse 3 University, faculty of biology
Sep. 2006 - Jun. 2007
BSc. in organisms, populations and ecosystems biology at the Toulouse 3 University, faculty of biology
Sep. 2004 - Jun. 2006
2 first year of BSc. in biology sciences at the University Paul Verlaine of Metz, faculty of biology/environment