Dr. Regina Stoltenburg 

Contact / Address


Dr. Regina Stoltenburg
Scientist

Department of Soil Ecology
Helmholtz Centre for Environmental Research - UFZ
Theodor-Lieser-Str. 4 | D-06120 Halle/Saale
Germany

Tel.:
Email:
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Regina Stoltenburg

Current Research Interests

Microbial diversity – the invisible variability
  • microbial communities in the environment and in biotechnological processes
  • dynamics and interactions of microbial communities
  • use of molecular methods, metagenomics
Microbial Diversity, Grafik: R. Stoltenburg/UFZ
Genomic G-quadruplexes (G4)
  • special structures of G-rich DNA or RNA
  • high structural diversity (topologies)
  • potential role in gene regulation and genome stability
G-Quadruplex, Grafik: R. Stoltenburg/UFZ

Further Research Interests


Aptamers – artificial, highly affine nucleic acid molecules

The functionality of aptamers is similar to that of antibodies. Aptamer are selected for specific target molecules by an in vitro selection and amplification method called SELEX. They can recognise and bind their targets with high affinity and specificity. As single-stranded oligonucleotides, aptamers are able to fold into complex and stable three-dimensional structures, which allow them to specifically interact with their targets. Aptamers are receiving increasing attention as alternative affinity reagents and represent essential tools in both basic and applied research. Aptamers can be used to detect and characterise their targets but also to modify the activity of their targets. Therefore, they provide a broad range of applications, e.g., affinity enrichment, analytics, medical diagnostics, or therapy.

  • new sequencing technologies (NGS) and bioinformatic analysis as part of the optimisation of the aptamer selection process
  • selection of new DNA aptamers for various targets such as peptides/proteins, small organic molecules or complex structures/target mixtures

    (streptavidin, Tau peptide, toxins, pharmaceuticals, bacterial LPS, and bacterial Protein A:  original aptamer selection / expanding the aptamer portfolio )

  • characterisation and post-SELEX modification/optimisation of selected aptamers

Yeast Genetics
  • Arxula adeninivorans (new Blastobotrys adeninivorans), Candida utilis as non-conventional yeasts: genetic diversity of C. utilis, screening/cloning of specific genes of A. adeninivorans, heterologous gene expression
  • S. cerevisiae: protein secretion/transport using secretory mutants

Research Projects

  • International Water Research Alliance Saxony (IWAS I, II), subproject: Development of a multi-sensor system for the detection of pathogenic microorganisms
    BMBF joint research project (02WM1027, 02WM1165), project term: 2008 – 2012
    Cooperation: TU Dresden / Fraunhofer IWS Dresden
  • Portable measurement system for the detection of pharmaceutical residues in waters, subproject: Aptamers for pharmaceutical residues
    AiF PRO INNO II (KF0011004DA6), project term: 2006 – 2008
    Cooperation: AMTEC Analysenmesstechnik GmbH
  • New approaches for diagnosis of sporadic Alzheimer's disease and new variant Creutzfeldt-Jakob disease, subproject: Development of aptamers
    SMWK / EFRE (4-0123.55-20-0361-04/8, project no.: 4212/04-14), project term: 2004 – 2006
    Cooperation: Leipzig University (Coordination), Labor Diagnostik GmbH
  • Development of aptamers for biosensors
    SMUL (13-8811.61/93), project term: 2001 – 2003
    Cooperation: Ingenieurbüro für Verpackung (IBV), Dresden
  • New biosensors for the determination of halogenated organics
    GIF (I 0442-168.03./95), project term: 1999 – 2000
    Cooperation: Hebrew University in Jerusalem, University of Stuttgart

Curriculum Vitae

since 2014

Scientist at the Helmholtz Centre for Environmental Research – UFZ in Halle (Dept. Soil Ecology)

2006 - 2014

Scientist at the Helmholtz Centre for Environmental Research – UFZ in Leipzig (Dept. UBZ, Research Group: Aptamers and Applications)

2004 - 2006

Scientist at the Leipzig University (Faculty of Chemistry and Mineralogy, Bioanalysis, BBZ)

1999 - 2004

Scientist at the Helmholtz Centre for Environmental Research – UFZ in Leipzig (Dept. UBZ, Research Group: Aptamers and Applications)

1996 - 1999

Scientist at IPK Gatersleben (Research Group: Yeast Genetics)

1992 - 1996

PhD student at The Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben (Research Group: Yeast Genetics)
PhD in Biochemistry at the EMA University of Greifswald.
Thesis: „Untersuchungen zu einem Sec1p- und Sec7p-unabhängigen Proteintransportweg bei Saccharomyces cerevisiae am Beispiel des Glykoproteins gp115”

1992

Diploma in Biology (focus: Microbiology) at the Ernst Moritz Arndt University (EMAU) of Greifswald including a partial study at the RWTH Aachen University, Institute of Biology IV.
Thesis: „Molekularbiologische Untersuchungen zur Diversität der Hefe Candida utilis


Selected Publications

Stoltenburg, R., Strehlitz, B. (2018): Refining the Results of a Classical SELEX Experiment by Expanding the Sequence Data Set of an Aptamer Pool Selected for Protein A. Int. J. Mol. Sci. 2018, 19, 642. DOI: 10.3390/ijms19020642  Journal

Reich, P., Stoltenburg, R., Strehlitz, B., Frense, D., Beckmann, D. (2017): Development of An Impedimetric Aptasensor for the Detection of Staphylococcus aureus. Int. J. Mol. Sci. 18: 2484. DOI: 10.3390/ijms18112484  Journal

Stoltenburg, R.
, Krafčiková, P., Víglaský, V., Strehlitz, B. (2016): G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports 6: 33812. DOI: 10.1038/srep33812  Journal

Stoltenburg, R., Schubert, T., Strehlitz, B. (2015): In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein A. PLoS ONE 10 (7): e0134403. DOI: 10.1371/journal.pone.0134403  Journal

Heilkenbrinker, A., Reinemann, C., Stoltenburg, R., Walter, J.-G., Jochums, A., Stahl, F., Zimmermann, S., Strehlitz, B., Scheper, T. (2015): Identification of the Target Binding Site of Ethanolamine-Binding Aptamers and Its Exploitation for Ethanolamine Detection. Anal. Chem. 87: 677-685. DOI: 10.1021/ac5034819  Journal

Stoltenburg, R., Nikolaus, N., Strehlitz, B. (2012): Capture-SELEX: selection of DNA aptamers for aminoglycoside antibiotics. J. Anal. Methods Chem. 2012: 415697. DOI:10.1155/2012/415697  Journal

*Reinemann, C., *Stoltenburg, R., Strehlitz, B. (2009): Investigations on the Specificity of DNA Aptamers Binding to Ethanolamine. Anal. Chem. 81: 3973-3978. DOI: 10.1021/ac900305y  Journal
(*gleichberechtigte Autorenschaft)

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2007): SELEX - a (r)evolutionary method to generate high affinity nucleic acid ligands. Biomol. Eng. 24: 381-403. DOI: 10.1016/j.bioeng.2007.06.001  Journal

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2005): FluMag-SELEX as an advantageous method for DNA aptamer selection. Anal. Bioanal. Chem. 383: 83-91. DOI: 10.1007/s00216-005-3388-9  Journal

Complete List of Publications and Patents

Stoltenburg, R., Strehlitz, B. (2018): Refining the Results of a Classical SELEX Experiment by Expanding the Sequence Data Set of an Aptamer Pool Selected for Protein A. Int. J. Mol. Sci. 2018, 19, 642. DOI: 10.3390/ijms19020642
Journal

Reich, P., Stoltenburg, R., Strehlitz, B., Frense, D., Beckmann, D. (2017): Development of An Impedimetric Aptasensor for the Detection of Staphylococcus aureus. Int. J. Mol. Sci. 18: 2484. DOI: 10.3390/ijms18112484
Journal

Stoltenburg, R., Krafčiková, P., Víglaský, V., Strehlitz, B. (2016): G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports 6: 33812. DOI: 10.1038/srep33812 
Journal

Stoltenburg, R., Schubert, T., Strehlitz, B. (2015): In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein A. PLoS ONE 10 (7): e0134403. DOI: 10.1371/journal.pone.0134403
Journal

Online-Presentation of the article "In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein A" (PLoS ONE 2015, 10(7):e0134403) by Global Medical Discovery, 02-Nov-2015.
Website

Heilkenbrinker, A., Reinemann, C., Stoltenburg, R., Walter, J.-G., Jochums, A., Stahl, F., Zimmermann, S., Strehlitz, B., Scheper, T. (2015): Identification of the Target Binding Site of Ethanolamine-Binding Aptamers and Its Exploitation for Ethanolamine Detection. Anal. Chem. 87: 677-685. DOI: 10.1021/ac5034819
Journal

Tröger, V., Strehlitz, B., Stoltenburg, R., Schmieder, S., Sonntag, F. (2013): Charakterisierung von Protein A-spezifischen DNA-Aptameren mittels SPR. 11. Dresdner Sensor-Symposium, December 2013, Dresden, Germany. Conference paper, Chapter: 1. Biosensorik und Point of Care: 178-182. DOI: 10.5162/ 11dss2013/A12
Conference Website

Strehlitz, B., Reinemann, C., Linkorn, S., Stoltenburg, R. (2012): Aptamers for pharmaceuticals and their application in environmental analytics. Bioanal. Rev. 4: 1-30. DOI: 10.1007/s12566-011-0026-1
Journal

Stoltenburg, R., Nikolaus, N., Strehlitz, B. (2012): Capture-SELEX: selection of DNA aptamers for aminoglycoside antibiotics. J. Anal. Methods Chem. 2012: 415697. DOI:10.1155/2012/415697
Journal

Ruigrok, V., van Duijn, E., Barendregt, A., Dyer, K., Tainer, J., Stoltenburg, R., Strehlitz, B., Levisson, M., Smidt, H., van der Oost, J. (2012): Selection of streptavidin binding aptamers and their kinetic and stoichiometric characterization. ChemBioChem. 13 (6): 829-836. DOI: 10.1002/cbic.201100774
Journal

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2010): Erkennung von Umweltschadstoffen durch DNA-Aptamere. Laborwelt. 11. Jahrgang, Nr. 2 / 2010: 6-9.
Journal

*Reinemann, C., *Stoltenburg, R., Strehlitz, B. (2009): Investigations on the Specificity of DNA Aptamers Binding to Ethanolamine. Anal. Chem. 81: 3973-3978. DOI: 10.1021/ac900305y
(*gleichberechtigte Autorenschaft)
Journal
 
Strehlitz, B., Nikolaus, N., Stoltenburg, R. (2008): Protein Detection with Aptamer Biosensors. Sensors 8: 4296-4307. DOI: 10.3390/s8074296
Journal

Strehlitz, B., Stoltenburg, R. (2008): Neue Methoden zur Schadstoff-Analytik. Ingenieur-Nachrichten. Ausgabe 02/2008: 5.

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2007): SELEX - a (r)evolutionary method to generate high affinity nucleic acid ligands. Biomol. Eng. 24: 381-403. DOI: 10.1016/j.bioeng.2007.06.001
Journal

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2006): DNA Aptamer Selection Using a Ligand Evolution Process. American Biotechnology Laboratory. January, Vol. 24, No. 1: 18-20.

Mann, D., Reinemann, C., Stoltenburg, R., Strehlitz, B. (2005): In vitro selection of DNA aptamers binding ethanolamine. Biochem. Biophys. Res. Commun. 338: 1928-1934. DOI: 10.1016/j.bbrc2005.10.172
Journal

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2005): FluMag-SELEX as an advantageous method for DNA aptamer selection. Anal. Bioanal. Chem. 383: 83-91. DOI: 10.1007/s00216-005-3388-9
Journal

Stoltenburg, R., Strehlitz, B. (2003): Entwicklung von Aptameren für Biosensoren. UFZ-Bericht Nr. 13/2003, ISSN 0948-9452.

Wartmann, T., Stoltenburg, R., Böer, E., Sieber, H., Bartelsen, O., Gellissen, G. and Kunze, G. (2003): The ALEU2 gene – a new component for an Arxula adeninivorans based expression platform. FEMS Yeast Research 3: 223-232. DOI: 10.1016/S1567-1356(02)00190-3
Journal

Wartmann, T., Stephan, U.W., Bube, I., Böer, E., Melzer, M., Manteuffel, R., Stoltenburg, R., Guengerich, L., Gellissen, G. and Kunze, G. (2002): Post-translational modifications of the AFET3 gene product – a component of the iron transport system in budding cells and mycelia of the yeast Arxula adeninivorans. Yeast 19: 849-862. DOI: 10.1002/yea.880
Journal

Yang, X.-X., Wartmann, T., Stoltenburg, R., Kunze, G. (2000): Halotolerance of the yeast Arxula adeninivorans LS3. Antonie van Leeuwenhoek 77: 303-311. DOI: 10.1023/A:1002636606282
Journal

Kunze, I., Hensel, G., Adler, K., Bernard, J., Neubohn, B., Nilsson, C., Stoltenburg, R., Kohlwein, S.D., Kunze, G. (1999): The green fluorescent protein targets secretory proteins to the yeast vacuole. Biochim. Biophys. Acta 1410: 287-298. DOI: 10.1016/S0005-2728(99)00006-7
Journal

Stoltenburg, R., Lösche, O., Klappach, G. and Kunze, G. (1999): Molecular cloning and expression of the ARFC3 gene, a component of the replication factor C from the salt tolerant, dimorphic yeast Arxula adeninivorans LS3. Curr. Genet. 35: 8-13. DOI: 10.1007/s002940050426
Journal

Kühnel, C., Stoltenburg, R., Kunze, I. and Kunze, G. (1996): Long-term effects of restrictive culture conditions on Saccharomyces cerevisiae sec7 cells. Microbiol. Res. 151: 93-97. Doi: 10.1016/S0944-5013(96)80062-0
Journal

Stoltenburg, R., Wartmann, T., Kunze, I. and Kunze, G. (1995): Reliable method to prepare RNA from free and membrane-bound polysomes from different yeast species. BioTechniques 18: 564-568.
Journal

Stoltenburg, R., Klinner, U., Ritzerfeld, P., Zimmermann, M. und Emeis, C.C. (1992): Genetic diversity of the yeast Candida utilis. Curr. Genet. 22: 441-446. DOI: 10.1007/BF00326408
Journal

Strehlitz, B., Stoltenburg, R. (2009): SELEX and its Recent Optimizations. In: Mascini, M., (Ed.), Aptamers in Bioanalysis. John Wiley & Sons, Inc., pp. 31-59.
Website

Stoltenburg, R., Reinemann, C., Strehlitz, B. (2007): Development of Aptamers as New Receptors for Environmental Biosensors. In: Mishra, C.S.K. and Juwarkar, A.A., (Ed.), Environmental Biotechnology. APH Publishing Corporation, New Delhi, pp. 83-112.

Reinemann, C., Strehlitz, B., Stoltenburg, R.: Fluorchinolon-spezifische Aptamere. Deutsches Patent DE 102013 204 057.1, Anmeldetag: 08.03.2013, Deutsches Patent- und Markenamt, München.
Internationales Patent EP2774988B1, Anmeldetag: 04.03.2014, Europäisches Patentamt.

Nikolaus, N., Prange, A., Stoltenburg, R., Strehlitz, B.: Aminoglycosid spezifische Aptamere. Deutsches Patent DE 102011006612.8, Anmeldetag: 31.03.2011, Deutsches Patent- und Markenamt, München.
Internationales Patent EP2691523, Anmeldetag: 29.03.2012, Europäisches Patentamt.

Stoltenburg, R., Strehlitz, B.: Aptamere, die spezifisch sind für Immunglobulin bindende Zellwandproteine. Deutsches Patent DE 102011006610.1, Anmeldetag: 31.03.2011, Deutsches Patent- und Markenamt, München.
Internationales Patent PCT/EP2012/055655, Anmeldetag: 29.03.2012, Europäisches Patentamt.
US-Patent 9,353,421 B2, Anmeldetag: 29.03.2012, United States Patent and Trademark Office (USPTO).

Stoltenburg, R., Strehlitz, B., Reinemann, C.: Tau-Protein spezifische Aptamere, deren Verwendung sowie ein Kit umfassend solche Aptamere. Deutsches Patent DE 10 2010 038 842.4, Anmeldetag: 03.08.2010, Deutsches Patent- und Markenamt, München.

Strehlitz, B., Mann, D., Reinemann, C., Stoltenburg, R.: Ethylaminspezifische Aptamere, deren Verwendung und ein Kit umfassend diese Aptamere. Deutsches Patent DE 10 2005 052 275.0, Anmeldetag: 27.10.2005, Deutsches Patent- und Markenamt, München.

Strehlitz, B., Stoltenburg, R.Gründig, B.: Elektrochemischer Nachweis von Nukleinsäuren. Deutsches Patent DE 102 56 898.7, Anmeldetag: 29.11.2002, Deutsches Patent- und Markenamt, München.

Kunze, G., Walter, I., Rösel, H., Böer, E., Stoltenburg, R., Wartmann, T., Bui, M.D.: Method for synthesizing proteins in a yeast of the genus Arxula and suitable promoters. US Patent US 2003/0186376, Anmeldetag: 08.11.2002.

Kunze, G., Walter, I., Rösel, H., Böer, E., Stoltenburg, R., Wartmann, T., Bui, MD.: Verfahren zum Herstellen von Proteinen in einer Hefe der Gattung Arxula und dafür geeignete Promotoren. Deutsches Patent DE 501 15 564.3, Anmeldetag: 08.05.2001, Deutsches Patent- und Markenamt, München.
Internationales Patent PCT/EP2001/005225, Anmeldetag: 08.05.2001, Europäisches Patentamt.