Dr. Regina Stoltenburg 

Contact / Address


Dr. Regina Stoltenburg
Scientist

Department of Soil Ecology
Helmholtz Centre for Environmental Research - UFZ
Theodor-Lieser-Str. 4 | D-06120 Halle/Saale
Germany

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Regina Stoltenburg

Current Research Interests

Microbial diversity – the invisible variability
  • structural and functional diversity of microbial soil organisms, their dynamics and interactions
  • effects of different land use concepts and environmental changes on the microbial community in soil
  • use of cultivation-dependent techniques and molecular methods (genomics, transcriptomics)
  • G-quadruplexes as regulatory elements
Microbial Diversity, Grafik: R. Stoltenburg/UFZ

Further Research Interests

Aptamers – artificial, highly affine nucleic acid molecules

The functionality of aptamers is similar to that of antibodies. Aptamer are selected for specific target molecules by an in vitro selection and amplification method called SELEX. They can recognise and bind their targets with high affinity and specificity. As single-stranded oligonucleotides, aptamers are able to fold into complex and stable three-dimensional structures, which allow them to specifically interact with their targets. Aptamers are receiving increasing attention as alternative affinity reagents and represent essential tools in both basic and applied research. Aptamers can be used to detect and characterise their targets but also to modify the activity of their targets. Therefore, they provide a broad range of applications, e.g., affinity enrichment, analytics, medical diagnostics, or therapy.

  • new sequencing technologies (NGS) and bioinformatic analysis as part of the optimisation of the aptamer selection process
  • selection of new DNA aptamers for various targets such as peptides/proteins, small organic molecules or complex structures/target mixtures

    (streptavidin, Tau peptide, toxins, pharmaceuticals, bacterial LPS, bacterial Protein A )

  • characterisation and post-SELEX modification/optimisation of selected aptamers
  • development and application of aptamer-based assays using different detection principles (fluorescence-based assays, ELONA, Biacore, IAsys, MST), applying own and well-known aptamers such as aptamers for thrombin or cocaine