1 High-throughput cell-based bioassays

The CITEPro module “High-throughput cell-based bioassays” is used for the testing of single chemicals, mixtures and environmental samples with a battery of reporter gene assays and high-content microscopy using mammalian and fish cells. Diverse pipetting robots and dispensers (1-3) are used to prepare the experiments and dose the samples. Stringent quality control is assured by routinely monitoring cell confluency by an Incucyte S3 microscope (5). Detection of reporter proteins is performed with a plate reader (6) automated imaging with advanced image analysis, this method allows for the simultaneous measurement of multiple cellular parameters (morphology, viability) and functional imaging (mitochondrial activity and genotoxicity) as well as cell painting (7).

Application: Assessment of cytotoxicity and specific modes of action in vitro

Selected literature references
 

Lee, J., et al. (2024). "Water Quality Monitoring with the Multiplexed Assay MitoOxTox for Mitochondrial Toxicity, Oxidative Stress Response, and Cytotoxicity in AREc32 Cells." Environmental Science & Technology 58(13): 5716-5726.

Neale, P. A.; Braun, G.; Brack, W.; Carmona, E.; Gunold, R.; Konig, M.; Krauss, M.; Liebmann, L.; Liess, M.; Link, M.; Schafer, R. B.; Schlichting, R.; Schreiner, V. C.; Schulze, T.; Vormeier, P.; Weisner, O.; Escher, B. I., "Assessing the Mixture Effects in In Vitro Bioassays of Chemicals Occurring in Small Agricultural Streams during Rain Events". Environ. Sci. Technol. 2020, 54, 8280-8290; DOI 10.1021/acs.est.0c02235.

Braun, G., Herberth, G., Krauss, M., König, M., Wojtysiak, N., Zenclussen, A. C. & Escher, B. I. "Neurotoxic Mixture Effects of Chemicals Extracted from Blood of Pregnant Women." Science 2024, 386, 301-309 (). DOI 10.1126/science.adq0336