Dr. Nils Klüver
Helmholtz Zentrum für
Umweltforschung - UFZ
Department Bioanalytische Ökotoxikologie
Permoserstr. 15
04318 Leipzig
Building: 6.0
Room: 520
Phone: +49 341 6025 1558
Forschungsinteressen
- Mechanistische Toxikologie
- Molekular- und Entwicklungsbiologie
- Entwicklungsneurotoxizität von Chemikalien unter der Berücksichtigung subletalen Endpunkten, z. B. Bewegungsmustern, Veränderung der Morphologie und der Genexpression
- toxikokinetische und toxikodynamische Prozesse, um die beobachteten Wirkungen mit den internen Konzentrationen im Zebrabärblingsembryo in Zusammenhang zu bringen.
- Verbesserungen und Erhöhung der Akzeptanz des Zebrabärblingsembryo-Modells als Alternative zu Tierversuchen in in-vitro Teststartgien für die Risiko- und Gefahrenbewertung.
Lebenslauf
Seit 01/2019
Wissenschaftler, Department Bioanalytische Ökotoxikologie, Helmholtz-Zentrum für Umweltforschung – UFZ, Leipzig
01/2015 - 12/2018
PostDoc, Department Zelltoxikologie, Helmholtz-Zentrum für Umweltforschung – UFZ, Leipzig
07/2008 - 12/2014
PostDoc, Department Bioanalytische Ökotoxikologie, Helmholtz-Zentrum für Umweltforschung – UFZ, Leipzig
04/2008 - 07/2008
PostDoc, Physiologischen Chemie I, Julius-Maximilians-Universität Würzburg
09/2007 - 03/2008
PostDoc, Fachbereich Biologische Wissenschaften (Abteilung Stammzellbiologie), Nationale Universität Singapur, Singapur
04/2003 - 08/2007
Anfertigung der Dissertation am Institut der Physiologischen Chemie I, Julius-Maximilians-Universität Würzburg
Abschlussarbeit: “Molecular mechanisms of gonad development in Medaka and Oryzias celebensis”
Abschluss: Dr. rer. nat.
10/1999 - 01/2033
Biologie Studium mit Abschluss Diplom, Julius-Maximilians-Universität Würzburg
Abschlussarbeit: "Functional analysis of lamin-mutants in vivo"
10/1997 - 12/1999
Grundstudium der Biologie , Philipps-Universität Marburg
Auszeichnungen
2012
EPAA 3Rs Science Award
2012
CrackIT Award
04/2004 - 07/2007
Doktorandenstipendium der DFG Graduierten Schule 1048
Graduiertenschule für Biowissenschaften, Julius-Maximilians-Universität Würzburg
Publikationen
Inhalt:
Weiterführende Recherchen können Sie in unserem Publikationsverzeichnis durchführen.
2025 (2)
- Bertram, M.G., Ågerstrand, M., Thoré, E.S.J., Allen, J., Balshine, S., Brand, J.A., Brooks, B.W., Dang, Z., Duquesne, S., Ford, A.T., Hoffmann, F., Hollert, H., Jacob, S., Kloas, W., Klüver, N., Lazorchak, J., Ledesma, M., Maack, G., Macartney, E.L., Martin, J.M., Melvin, S.D., Michelangeli, M., Mohr, S., Padilla, S., Pyle, G., Saaristo, M., Sahm, R., Smit, E., Steevens, J.A., van den Berg, S., Vossen, L.E., Wlodkowic, D., Wong, B.B.M., Ziegler, M., Brodin, T. (2025):
EthoCRED: a framework to guide reporting and evaluation of the relevance and reliability of behavioural ecotoxicity studies
Biol. Rev. 100 (2), 556 - 585 10.1111/brv.13154 - Michaelis, P., Klüver, N., Aulhorn, S., Bohring, H., Bumberger, J., Haase, K., Kuhnert, T., Küster, E., Krüger, J., Luckenbach, T., Massei, R., Nerlich, L., Petruschke, S., Schnicke, T., Schnurpel, A., Scholz, S., Schweiger, N., Sielaff, D., Busch, W. (2025):
Leveraging zebrafish embryo phenotypic observations to advance data-driven analyses in toxicology
Environ. Sci. Technol. 59 (9), 4304 - 4317 10.1021/acs.est.4c11757
2024 (5)
- Escher, B.I., Ahlheim, J., Böhme, A., Borchardt, D., Brack, W., Braun, G., Colbourne, J.K., Dann, J.P., Gessner, J., Jahnke, A., König, M., Klüver, N., Krauss, M., Lee, J., Li, X., Lips, S., Orsini, L., Rinke, K., Schmitt-Jansen, M., Scholz, S., Schulze, T., Spahr, S., Ulrich, N., Weitere, M., Varga, E. (2024):
Mixtures of organic micropollutants exacerbated in vitro neurotoxicity of prymnesins and contributed to aquatic toxicity during a toxic algal bloom
Nat. Water 2 (9), 889 - 898 10.1038/s44221-024-00297-4 - Gutsfeld, S., Wehmas, L., Omoyeni, I.E., Schweiger, N., Leuthold, D., Michaelis, P., Howey, X.M., Gaballah, S., Herold, N., Vogs, C., Wood, C., Bertotto, L., Wu, G.-M., Klüver, N., Busch, W., Scholz, S., Schor, J., Tal, T. (2024):
Investigation of peroxisome proliferator-activated receptor genes as requirements for visual startle response hyperactivity in larval zebrafish exposed to structurally similar per- and polyfluoroalkyl substances (PFAS)
Environ. Health Perspect. 132 (7), art. 077007 10.1289/EHP13667 - Kramer, L., Schulze, T., Klüver, N., Altenburger, R., Hackermüller, J., Krauss, M., Busch, W. (2024):
Curated mode-of-action data and effect concentrations for chemicals relevant for the aquatic environment
Sci. Data 11 , art. 60 10.1038/s41597-023-02904-7 - Michaelis, P., Klüver, N., Busch, W. (2024):
Integrated Effect Database for Toxicological Observations (INTOB) [Data set]
Zenodo 10.5281/zenodo.11030299 - Tal, T., Myhre, O., Fritsche, E., Rüegg, J., Craenen, K., Aiello-Holden, K., Agrillo, C., Babin, P.J., Escher, B.I., Dirven, H., Hellsten, K., Dolva, K., Hessel, E., Heusinkveld, H.J., Hadzhiev, Y., Hurem, S., Jagiello, K., Judzinska, B., Klüver, N., Knoll-Gellida, A., Kühne, B.A., Leist, M., Lislien, M., Lyche, J.L., Müller, F., Colbourne, J.K., Neuhaus, W., Pallocca, G., Seeger, B., Scharkin, I., Scholz, S., Spjuth, O., Torres-Ruiz, M., Bartmann, K. (2024):
New approach methods to assess developmental and adult neurotoxicity for regulatory use: a PARC work package 5 project
Front. Toxicol. 6 , art. 1359507 10.3389/ftox.2024.1359507
2023 (3)
- Bashirova, N., Poppitz, D., Klüver, N., Scholz, S., Matysik, J., Alia, A. (2023):
A mechanistic understanding of the effects of polyethylene terephthalate nanoplastics in the zebrafish (Danio rerio) embryo
Sci. Rep. 13 , art. 1891 10.1038/s41598-023-28712-y - Kramer, L., Schulze, T., Klüver, N., Altenburger, R., Hackermüller, J., Krauss, M., Busch, W. (2023):
Curated mode-of-action data and effect concentrations for chemicals relevant for the aquatic environment [Data set] v2
Zenodo 10.5281/zenodo.10071824 - Sanz, C.M., Schiavone, V., Ibarra, J., Di Donato, V., Truong, L., Tanguay, R., Hsieh, J.-H., Ryan, K., Ellis, L., Molina Martinez, B., Del Pozo, A., Alzualde, A., Muriana, A., Klüver, N., Behl, M., Woodland, C., Sachana, M., Hessel, E. (2023):
Towards developing a harmonized experimental protocol of embryonic zebrafish light/dark transition behavior test for Organisation for Economic Co-operation and Development (OECD) Developmental Neurotoxicity (DNT) In-Vitro Testing Battery
Toxicol. Lett. 384 (Suppl. 1), S249 - S249 10.1016/S0378-4274(23)00847-0
2022 (6)
- Alzualde, A., Hsieh, J.-H., Ellis, L., Schiavone, V., Truong, L., Legradi, J., Rubbini, D., Woodland, C., Klüver, N., Ryan, K., Behl, M., Terriente, J., Muriana, A., Tanguay, R., Sachana, M., Hill, B., Padilla, S., Shafer, T., Hessel, E. (2022):
An inter-laboratory case study to harmonize zebrafish light-dark transition test to predict developmental neurotoxicity for the OECD guidance document
Int. J. Toxicol. 41 (1), 55 - 56 10.1177/10915818211061894 - Henneberger, L., Mühlenbrink, M., Klüver, N., Escher, B. (2022):
Trout and human plasma protein binding of selected pharmaceuticals informs the fish plasma model
Environ. Toxicol. Chem. 41 (3), 559 - 568 10.1002/etc.4934 - Padilla, S., Hill, B.N., Legradi, J., Klüver, N. (2022):
Using zebrafish to assess developmental neurotoxicity
In: Gupta, R.C. (ed.)
Reproductive and developmental toxicology. Third edition
Academic Press / Elsevier, London, p. 239 - 251 10.1016/B978-0-323-89773-0.00013-8 - Sanz, C.M., Ibarra, J., Rubbini, D., Hsieh, J.-H., Ellis, L., Alzualde, A., Truong, L., Klüver, N., Muriana, A., Ryan, K., Behl, M., Molina, B., Legradi, J., Padilla, S., Woodland, C., Tanguay, R., Hill, B., Shafer, T., Sachana, M., Terriente, J., Di Donato, V., Schiavone, V., Hessel, E. (2022):
Zebrafish, a novel key player for human risk assessment: latest advances on developmental neurotoxicity from an international consortium
Toxicol. Lett. 368 (Suppl.), S235 - S236 10.1016/j.toxlet.2022.07.632 - Teixidó, E., Kieβling, T.R., Klüver, N., Scholz, S. (2022):
Grouping of chemicals into mode of action classes by automated effect pattern analysis using the zebrafish embryo toxicity test
Arch. Toxicol. 96 (5), 1353 - 1369 10.1007/s00204-022-03253-x - Thessen, A.E., Marvel, S., Achenbach, J.C., Fischer, S., Haendel, M.A., Hayward, K., Klüver, N., Könemann, S., Legradi, J., Lein, P., Leong, C., Mylroie, J.E., Padilla, S., Perone, D., Planchart, A., Miñana Prieto, R., Muriana, A., Quevedo, C., Reif, D., Ryan, K., Stinckens, E., Truong, L., Vergauwen, L., Vom Berg, C., Wilbanks, M., Yaghoobi, B., Hamm, J. (2022):
Implementation of zebrafish ontologies for toxicology screening
Front. Toxicol. 4 , art. 817999 10.3389/ftox.2022.817999
2021 (2)
- Ford, A.T., Ågerstrand, M., Brooks, B.W., Allen, J., Bertram, M.G., Brodin, T., Dang, Z., Duquesne, S., Sahm, R., Hoffmann, F., Hollert, H., Jacob, S., Klüver, N., Lazorchak, J.M., Ledesma, M., Melvin, S.D., Mohr, S., Padilla, S., Pyle, G.G., Scholz, S., Saaristo, M., Smit, E., Steevens, J.A., van den Berg, S., Kloas, W., Wong, B.B.M., Ziegler, M., Maack, G. (2021):
The role of behavioral ecotoxicology in environmental protection
Environ. Sci. Technol. 55 (9), 5620 - 5628 10.1021/acs.est.0c06493 - Teixidó, E., Klüver, N., Ogungbemi, A.O., Küster, E., Scholz, S. (2021):
Evaluation of neurotoxic effects in zebrafish embryos by automatic measurement of early motor behaviors
In: Llorens, J., Barenys, M. (eds.)
Experimental neurotoxicology methods
Neuromethods 172
Springer Nature, p. 381 - 397 10.1007/978-1-0716-1637-6_17
2020 (2)
- Escher, B.I., Abagyan, R., Embry, M., Klüver, N., Redman, A.D., Zarfl, C., Parkerton, T.F. (2020):
Recommendations for improving methods and models for aquatic hazard assessment of ionizable organic chemicals
Environ. Toxicol. Chem. 39 (2), 269 - 286 10.1002/etc.4602 - Fischer, F.C., Abele, C., Henneberger, L., Klüver, N., König, M., Mühlenbrink, M., Schlichting, R., Escher, B.I. (2020):
Cellular metabolism in high-throughput in vitro reporter gene assays and implications for the quantitative in vitro-in vivo extrapolation
Chem. Res. Toxicol. 33 (7), 1770 - 1779 10.1021/acs.chemrestox.0c00037
2019 (4)
- Bittner, L., Klüver, N., Henneberger, L., Mühlenbrink, M., Zarfl, C., Escher, B.I. (2019):
Combined ion-trapping and mass balance models to describe the pH-dependent uptake and toxicity of acidic and basic pharmaceuticals in zebrafish embryos (Danio rerio)
Environ. Sci. Technol. 53 (13), 7877 - 7886 10.1021/acs.est.9b02563 - Bittner, L., Teixidó, E., Keddi, I., Escher, B.I., Klüver, N. (2019):
pH‐Dependent uptake and sublethal effects of antihistamines in zebrafish (Danio rerio) embryos
Environ. Toxicol. Chem. 38 (5), 1012 - 1022 10.1002/etc.4395 - Klüver, N., Bittermann, K., Escher, B.I. (2019):
QSAR for baseline toxicity and classification of specific modes of action of ionizable organic chemicals in the zebrafish embryo toxicity test
Aquat. Toxicol. 207 , 110 - 119 10.1016/j.aquatox.2018.12.003 - Leuthold, D., Klüver, N., Altenburger, R., Busch, W. (2019):
Can environmentally relevant neuroactive chemicals specifically be detected with the locomotor response test in zebrafish embryos?
Environ. Sci. Technol. 53 (1), 482 - 493 10.1021/acs.est.8b04327
2018 (2)
- Bittner, L., Teixido, E., Seiwert, B., Escher, B.I., Klüver, N. (2018):
Influence of pH on the uptake and toxicity of β-blockers in embryos of zebrafish, Danio rerio
Aquat. Toxicol. 201 , 129 - 137 10.1016/j.aquatox.2018.05.020 - Sobanska, M., Scholz, S., Nyman, A.-M., Cesnaitis, R., Gutierrez Alonso, S., Klüver, N., Kühne, R., Tyle, H., de Knecht, J., Dang, Z., Lundbergh, I., Carlon, C., De Coen, W. (2018):
Applicability of the fish embryo acute toxicity (FET) test (OECD 236) in the regulatory context of Registration, Evaluation, Authorisation, and Restriction of Chemicals (REACH)
Environ. Toxicol. Chem. 37 (3), 657 - 670 10.1002/etc.4055
2017 (3)
- Baumer, A., Bittermann, K., Klüver, N., Escher, B.I. (2017):
Baseline toxicity and ion-trapping models to describe the pH-dependence of bacterial toxicity of pharmaceuticals
Environ. Sci.-Process Impacts 19 (7), 901 - 916 10.1039/c7em00099e - Escher, B.I., Baumer, A., Bittermann, K., Henneberger, L., König, M., Kühnert, C., Klüver, N. (2017):
General baseline toxicity QSAR for nonpolar, polar and ionisable chemicals and their mixtures in the bioluminescence inhibition assay with Aliivibrio fischeri
Environ. Sci.-Process Impacts 19 (3), 414 - 428 10.1039/c6em00692b - Escher, B.I., Hackermüller, J., Polte, T., Scholz, S., Aigner, A., Altenburger, R., Böhme, A., Bopp, S.K., Brack, W., Busch, W., Chadeau-Hyam, M., Covaci, A., Eisenträger, A., Galligan, J.J., Garcia-Reyero, N., Hartung, T., Hein, M., Herberth, G., Jahnke, A., Kleinjans, J., Klüver, N., Krauss, M., Lamoree, M., Lehmann, I., Luckenbach, T., Miller, G.W., Müller, A., Phillips, D.H., Reemtsma, T., Rolle-Kampczyk, U., Schüürmann, G., Schwikowski, B., Tan, Y.-M., Trump, S., Walter-Rohde, S., Wambaugh, J.F. (2017):
From the exposome to mechanistic understanding of chemical-induced adverse effects
Environ. Int. 99 , 97 - 106 10.1016/j.envint.2016.11.029
2016 (3)
- Klüver, N., Vogs, C., Altenburger, R., Escher, B.I., Scholz, S. (2016):
Development of a general baseline toxicity QSAR model for the fish embryo acute toxicity test
Chemosphere 164 , 164 - 173 10.1016/j.chemosphere.2016.08.079 - Scholz, S., Klüver, N., Kühne, R. (2016):
Analysis of the relevance and adequateness of using Fish Embryo Acute Toxicity (FET) Test Guidance (OECD 236) to fulfil the information requirements and addressing concerns under REACH. Report ECHA-UFZ contract ECHA/2014/341
Helmholtz Centre for Environmental Research - UFZ, Leipzig, 104 pp. - Shahid, M., Takamiya, M., Stegmaier, J., Middel, V., Gradl, M., Klüver, N., Mikut, R., Dickmeis, T., Scholz, S., Rastegar, S., Yang, L., Strähle, U. (2016):
Zebrafish biosensor for toxicant induced muscle hyperactivity
Sci. Rep. 6 , art. 23768 10.1038/srep23768
Inhalt:
Weiterführende Recherchen können Sie in unserem Publikationsverzeichnis durchführen.
2015 (1)
- Klüver, N., König, M., Ortmann, J., Massei, R., Paschke, A., Kühne, R., Scholz, S. (2015):
Fish embryo toxicity test: Identification of compounds with weak toxicity and analysis of behavioral effects to improve prediction of acute toxicity for neurotoxic compounds
Environ. Sci. Technol. 49 (11), 7002 - 7011 10.1021/acs.est.5b01910
2014 (2)
- Klüver, N., Ortmann, J., Paschke, H., Renner, P., Ritter, A.P., Scholz, S. (2014):
Transient overexpression of adh8a increases allyl alcohol toxicity in zebrafish embryos
PLOS One 9 (3), e90619 10.1371/journal.pone.0090619 - Scholz, S., Ortmann, J., Klüver, N., Léonard, M. (2014):
Extensive review of fish embryo acute toxicities for the prediction of GHS acute systemic toxicity categories
Regul. Toxicol. Pharmacol. 69 (3), 572 - 579 10.1016/j.yrtph.2014.06.004
2013 (2)
- Fischer, S., Klüver, N., Burkhardt-Medicke, K., Pietsch, M., Schmidt, A.-M., Wellner, P., Schirmer, K., Luckenbach, T. (2013):
Abcb4 acts as multixenobiotic transporter and active barrier against chemical uptake in zebrafish (Danio rerio) embryos
BMC Biol. 11 , art. 69 10.1186/1741-7007-11-69 - Scholz, S., Sela, E., Blaha, L., Braunbeck, T., Galay-Burgos, M., García-Franco, M., Guinea, J., Klüver, N., Schirmer, K., Tanneberger, K., Tobor-Kapłon, M., Witters, H., Belanger, S., Benfenati, E., Creton, S., Cronin, M.T.D., Eggen, R.I.L., Embry, M., Ekman, D., Gourmelon, A., Halder, M., Hardy, B., Hartung, T., Hubesch, B., Jungmann, D., Lampi, M.A., Lee, L., Léonard, M., Küster, E., Lillicrap, A., Luckenbach, T., Murk, A.J., Navas, J.M., Peijnenburg, W., Repetto, G., Salinas, E., Schüürmann, G., Spielmann, H., Tollefsen, K.E., Walter-Rohde, S., Whale, G., Wheeler, J.R., Winter, M.J. (2013):
A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment
Regul. Toxicol. Pharmacol. 67 (3), 506 - 530 10.1016/j.yrtph.2013.10.003
2012 (1)
- Büttner, A., Busch, W., Klüver, N., Giannis, A., Scholz, S. (2012):
Transcriptional responses of zebrafish embryos exposed to potential sonic hedgehog pathway interfering compounds deviate from expression profiles of cyclopamine
Reprod. Toxicol. 33 (2), 254 - 263 10.1016/j.reprotox.2011.12.010
2011 (2)
- Klüver, N., Yang, L., Busch, W., Scheffler, K., Scheffler, P., Strähle, U., Scholz, S. (2011):
Transcriptional response of zebrafish embryos exposed to neurotoxic compounds reveals a muscle activity dependent hspb11 expression
PLoS One 6 (12), e29063 10.1371/journal.pone.0029063 - Scholz, S., Büttner, A., Klüver, N., Guinea, J. (2011):
Fish embryos as alternative models for drug safety evaluation
In: Xu, J.J., Urban, L. (eds.)
Predictive toxicology in drug safety
Cambridge University Press, New York, p. 244 - 268 10.1017/CBO9780511779053.015
2009 (2)
- Klüver, N., Scheffler, K., Renner, P., Scholz, S. (2009):
Toxicogenomic response of azinphos-methyl treated zebrafish embryos and implication for the development of predictive models for chronic (fish) toxicity
Toxicol. Lett. 189 (Suppl. 1), S94 - S94 10.1016/j.toxlet.2009.06.284 - Scholz, S., Klüver, N. (2009):
Effects of endocrine disrupters on sexual, gonadal development in fish
Sex. Dev. 3 (2-3), 136 - 151 10.1159/000223078
2008 (2)
- Herpin, A., Fischer, P., Liedtke, D., Klüver, N., Neuner, C., Raz, E., Schartl, M. (2008):
Sequential SDF1a and b-induced mobility guides Medaka PGC migration
Dev. Biol. 320 (2), 319 - 327 10.1016/j.ydbio.2008.03.030 - Pala, I., Klüver, N., Thorsteinsdottir, S., Schartl, M., Coelho, M.M. (2008):
Expression pattern of anti-Müllerian hormone (amh) in the hybrid fish complex of Squalius alburnoides
Gene 410 (2), 249 - 258 10.1016/j.gene.2007.12.018
2007 (2)
- Herpin, A., Rohr, S., Riedel, D., Klüver, N., Raz, E., Schartl, M. (2007):
Specification of primordial germ cells in medaka (Oryzias latipes)
BMC Dev. Biol. 7 , art. 3 10.1186/1471-213X-7-3 - Klüver, N., Pfennig, F., Pala, I., Storch, K., Schlieder, M., Froschauer, A., Gutzeit, H.O., Schartl, M. (2007):
Differential expression of anti-Müllerian hormone (amh) and anti-Müllerian hormone receptor type II (amhrII) in the teleost medaka
Dev. Dyn. 236 (1), 271 - 281 10.1002/dvdy.20997
2005 (1)
- Klüver, N., Kondo, M., Herpin, A., Mitani, H., Schartl, M. (2005):
Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specific subfunctionalization
Dev. Genes Evol. 215 (6), 297 - 305 10.1007/s00427-005-0477-x