Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/S0021-9673(01)00687-2 |
| Title (Primary) | Liquid chromatography—mass spectrometry and liquid chromatography—tandem mass spectrometry determination of N-methylpyrrolidinone in riverine biofilms |
| Author | Headley, J.V.; Peru, K.M.; Friesen, D.A.; Neu, T.R. |
| Journal | Journal of Chromatography A |
| Year | 2001 |
| Department | FLOEK; GM |
| Volume | 917 |
| Issue | 1-2 |
| Page From | 159 |
| Page To | 165 |
| Language | englisch |
| Keywords | Riverine biofilms |
| Abstract | A rugged procedure utilizing reversed-phase liquid chromatography with positive-ion electrospray ionization mass spectrometry (LC–MS) along with tandem MS is described for the quantification and confirmation of N-methylpyrrolidinone (NMP) in methanolic extracts of riverine biofilm. The LC–MS method provided a 100-fold improvement in detection limits (2 ng g−1 with a repeatability of 80–95% based on triplicate analyses) compared to a conventional LC–UV detection procedure and was applicable to quantitative analysis of biofilm samples with little or no clean up. Under low-energy collision induced dissociation (CID) conditions (17 V, laboratory frame of reference, with argon as the collision gas), two product-ions of the [M+H]+ ion were formed at m/z 69 [MH-CH3NH2]+ and m/z 58 [MH-CH3NCH]+ with relative abundances of 30% and 5%, respectively. These CID transitions were used to demonstrate that biofilm uptake of a photocatalytically-generated mixture of NMP was rapid once acclimation was achieved. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6396 |
| Headley, J.V., Peru, K.M., Friesen, D.A., Neu, T.R. (2001): Liquid chromatography—mass spectrometry and liquid chromatography—tandem mass spectrometry determination of N-methylpyrrolidinone in riverine biofilms J. Chromatogr. A 917 (1-2), 159 - 165 |
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