Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1002/anie.8633090 |
Licence  |
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| Title (Primary) | Integrated electromicrobial production of itaconic acid from CO2 at liter-scale using Komagataella phaffii |
| Author | Izadi, P.
; Severinsen Zijlstra, M.M.; Kas, A.
; Haus, P.; Enzinger, V.; Lutz, G.; Ata, Ö.; Bachleitner, S.; Mattanovich, D.; Harnisch, F.
|
| Source Titel | Angewandte Chemie-International Edition |
| Year | 2026 |
| Department | MIBITECH |
| Page From | e8633090 |
| Language | englisch |
| Topic | T7 Bioeconomy |
| Supplements | Supplement 1 |
| Keywords | biosynthesis; electrochemical CO2 reduction; engineered Komagataella phaffii; integration; itaconic acid |
| Abstract |
Coupling electrochemical CO2 reduction showing
high selectivity with microbial biosynthesis offering metabolic
versatility offers a promising strategy for converting CO2
into value-added products. We present proof-of-concept of
electromicrobially producing the platform chemical itaconic acid (IA)
from CO2 using a robust yeast chassis. The hybrid systems integrates in a single reactor electrochemical CO2 reduction with microbial synthesis using a synthetic autotrophic Komagataella phaffii strain. Thereby CO2 serves as carbon source while electrochemically generated formate from CO2 provides reducing power to sustain microbial growth and drive IA production, reaching average titres of 110.1 mg·L−1.
While demonstrating the feasibility of directly coupling
electrochemical and microbial processes within a single reactor, we
reveal key challenges associated with integration, including limited
system performance, variability in product titers, anodic oxidation of
cathodic products, and constraints in energy efficiency. Despite these
current limitations, our results establish the operational feasibility
of integrated electromicrobial CO2 conversion using industrial yeast and highlight critical parameters for process intensification. |
| Izadi, P., Severinsen Zijlstra, M.M., Kas, A., Haus, P., Enzinger, V., Lutz, G., Ata, Ö., Bachleitner, S., Mattanovich, D., Harnisch, F. (2026): Integrated electromicrobial production of itaconic acid from CO2 at liter-scale using Komagataella phaffii Angew. Chem.-Int. Edit. , e8633090 10.1002/anie.8633090 |
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