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Title (Primary) Comparison of rumen and manure microbiomes and implications for the inoculation of anaerobic digesters
Author Ozbayram, E.G.; Ince, O.; Ince, B.; Harms, H.; Kleinsteuber, S.;
Journal Microorganisms
Year 2018
Department UMB;
Volume 6
Issue 1
Language englisch;
POF III (all) R31; T15;
Supplements https://www.mdpi.com/2076-2607/6/1/15/s1
Keywords ruminant microbiome; plant fiber fermentation; anaerobic digestion; bioaugmentation; hydrolytic bacteria; methanogenic archaea; mcrA gene; 16S rRNA gene; 454 amplicon sequencing
UFZ wide themes RU4;
Abstract Cattle manure is frequently used as an inoculum for the start-up of agricultural biogas plants or as a co-substrate in the anaerobic digestion of lignocellulosic feedstock. Ruminal microbiota are considered to be effective plant fiber degraders, but the microbes contained in manure do not necessarily reflect the rumen microbiome. The aim of this study was to compare the microbial community composition of cow rumen and manure with respect to plant fiber-digesting microbes. Bacterial and methanogenic communities of rumen and manure samples were examined by 454 amplicon sequencing of bacterial 16S rRNA genes and mcrA genes, respectively. Rumen fluid samples were dominated by Prevotellaceae (29%), whereas Ruminococcaceae was the most abundant family in the manure samples (31%). Fibrobacteraceae (12%) and Bacteroidaceae (13%) were the second most abundant families in rumen fluid and manure, respectively. The high abundances of fiber-degrading bacteria belonging to Prevotellaceae and Fibrobacteraceae might explain the better erformance of anaerobic digesters inoculated with rumen fluid. Members of the genus Methanobrevibacter were the predominant methanogens in the rumen fluid, whereas methanogenic communities of the manure samples were dominated by the candidate genus Methanoplasma. Our results suggest that inoculation or bioaugmentation with fiber-digesting rumen microbiota can enhance the anaerobic digestion of lignocellulosic biomass.
ID 19910
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19910
Ozbayram, E.G., Ince, O., Ince, B., Harms, H., Kleinsteuber, S. (2018):
Comparison of rumen and manure microbiomes and implications for the inoculation of anaerobic digesters
Microorganisms 6 (1), art. 15