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DOI 10.1002/pmic.201700082
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Title (Primary) Sulfated hyaluronan attenuates inflammatory signaling pathways in macrophages involving induction of antioxidants
Author Jouy, F.; Lohmann, N.; Wandel, E.; Ruiz-Gómez, G.; Pisabarro, M.T.; Beck-Sickinger, A.G.; Schnabelrauch, M.; Möller, S.; Simon, J.C.; Kalkhof, S.; von Bergen, M.; Franz, S.
Source Titel Proteomics
Year 2017
Department MOLSYB
Volume 17
Issue 10
Page From art. 1700082
Language englisch
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Keywords Anti-inflammation; Antioxidants; Glycosaminoglycans; Macrophages; Sulfated hyaluronan
UFZ wide themes RU3;
Abstract It is well recognized that high molecular weight hyaluronan (H-HA) exerts potent anti-inflammatory effects while its fragmentation into low molecular weight HA (L-HA) is discussed to promote inflammation. Chemical modification of HA with sulfate groups has been shown to foster its anti-inflammatory activity which seems to be maintained in sulfated low molecular weight HA derivatives (sL-HA). However, the molecular mechanisms by which sL-HA produces its anti-inflammatory activity are not understood. In this study, we used global quantitative proteomics combined with targeted analysis of key proteins to characterize the effect of sL-HA on fully differentiated human inflammatory macrophages (iMФ). Culture of iMФ with sL-HA did not affect cell viability but resulted in a reduced pro-inflammatory cytokine response of iMФ after activation indicating a profound counter-regulation of their initial inflammatory phenotype. Rapid internalization of sL-HA involving CD44 and scavenger receptors was observed. Furthermore, an upregulation of the antioxidants SOD2 and SOD3 was found while no oxidative stress was induced. Consequently, activity of transcription factors for inflammatory gene expression was downregulated in iMФ with sL-HA after activation whereas anti-inflammatory proteins were induced.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19015
Jouy, F., Lohmann, N., Wandel, E., Ruiz-Gómez, G., Pisabarro, M.T., Beck-Sickinger, A.G., Schnabelrauch, M., Möller, S., Simon, J.C., Kalkhof, S., von Bergen, M., Franz, S. (2017):
Sulfated hyaluronan attenuates inflammatory signaling pathways in macrophages involving induction of antioxidants
Proteomics 17 (10), art. 1700082