Head of Department - Helmholtz-Centre for Environmental Research

Head of Department and
Group Leader Photobiotechnolgy

Prof. Dr. rer. nat. Andreas Schmid

Tel.: +49 341-6025 1246

Mail: Andreas Schmid

Research Interests

Photobiotechnology, single cell analysis, nano- and microreactors, catalytic biofilms, (continuous) redox biocatalysis using whole cells and isolated enzymes, highly productive and specific oxyfunctionalizations of hydrocarbons by means of microbial cells, systems biotechnology in Escherichia coli, Pseudomonas putida and yeast, sustainability.

Scientific Career

Education

2004		Habilitation, Swiss Federal Institute of Technology (ETHZ); venia legendi for microbiology and biotechnology
1996 - 1998		Postdoctoral Research Associate, Institute of Biotechnology, Swiss Federal Institute of Technology (ETHZ), Zurich, Switzerland; Area: Biocatalysis, project acquisitions from chemical & pharmaceutical companies, Evaluation 'Spinoff Company' in the field of biocatalysis / chemical process development.
1997		Dr. rer. nat., University of Stuttgart, Stuttgart, Germany (Prof. Dr. K.-H. Engesser and Prof. Dr. H. J. Knackmuss)
1992		Dipl.-Biol. (microbiology, biochemistry) University of Stuttgart, Stuttgart, Germany

Positions

since 2014		Head of Department Solar Materials at the Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
2004 - 2014		Professor, Chair, Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, Dortmund, Germany
2005 - 2011		Head Single Cell Laboratory at Leibniz Institut für Analytische Wissenschaften ISAS-e.V., Dortmund, Germany
2011		Visiting professor, AIBN, University of Queensland (Brisbane, Australia)
since 2006		Coordinator of 'ChemBioTec' (www.chembiotec.de), research network of Deutsche Bundesstiftung Umwelt
2006		Visiting professor (JSPS, Japan)
1999 - 2004		Group Leader, Head of ‘Technical Enzymology' Laboratory, Institute of Biotechnology (IBT, Prof. Dr. Bernard Witholt), Swiss Federal Institute of Technology, ETH Hoenggerberg, Zurich, Switzerland
1996 – 1998		Post Doctoral Fellow Institute of Biotechnology, ETH Hönggerberg, Zürich, Switzerland. with Prof. Dr. B. Witholt
1995 – 1996		Visiting Scientist Department of Microbiology, Swiss Federal Institute for Environmental Science and Technology, EAWAG, Dübendorf, Switzerland. with Dr. J. R. v.d. Meer, Prof. A. Zehnder
1994 - 1995		Staff scientist, Department of Biological Waste Air Treatment, Institute for Sanitary Engineering, Water Quality and Solid Waste Management (ISWA), University of Stuttgart, Stuttgart, Germany
1992 - 1994		PhD Student, Institute of Microbiology, University of Stuttgart, Germany
1988 - 1992		Research Assistent (HiWi), Institute of Microbiology, University of Stuttgart, Fraunhofer Society IGB, Stuttgart, Germany

Publications

You could use our publication index for further requests.

2021 (9)

Brandenburg, F., Theodosiou, E., Bertelmann, C., Grund, M., Klähn, S., Schmid, A., Krömer, J.O. (2021):
Trans-4-hydroxy-L-proline production by the cyanobacterium Synechocystis sp. PCC 6803
Metab. Eng. Commun. 12 , e00155 10.1016/j.mec.2020.e00155
Bühler, K., Bühler, B., Klähn, S., Krömer, J.O., Dusny, C., Schmid, A. (2021):
Biocatalytic production of white hydrogen from water using cyanobacteria
In: Rögner, M. (ed.)
Photosynthesis: Biotechnological applications with microalgae
De Gruyter, Berlin ; Boston, p. 279 - 306 10.1515/9783110716979-011
Bühler, K., Krömer, J.O., Klähn, S., Bühler, B., Dusny, C., Schmid, A. (2021):
Weißer Wasserstoff made in Leipzig. White hydrogen made in Leipzig
Biospektrum 27 (3), 335 10.1007/s12268-021-1572-x
Dusny, C., Schmid, A. (2021):
The metabolic flux probe (MFP)—Secreted protein as a non-disruptive information carrier for ¹³C-based metabolic flux analysis
Int. J. Mol. Sci. 22 (17), art. 9438 10.3390/ijms22179438
Lai, B., Schneider, H., Tschörtner, J., Schmid, A., Krömer, J.O. (2021):
Technical-scale biophotovoltaics for long-term photo-current generation from Synechocystis sp. PCC6803
Biotechnol. Bioeng. 118 (7), 2637 - 2648 10.1002/bit.27784
Lai, B., Schneider, H., Tschörtner, J., Schmid, A., Krömer, J.O. (2021):
Inside front cover image, Volume 118, Number 7, July 2021
Biotechnol. Bioeng. 118 (7), ii - ii 10.1002/bit.27411
Lupacchini, S., Appel, J., Stauder, R., Bolay, P., Klähn, S., Lettau, E., Adrian, L., Lauterbach, L., Bühler, B., Schmid, A., Toepel, J. (2021):
Rewiring cyanobacterial photosynthesis by the implementation of an oxygen-tolerant hydrogenase
Metab. Eng. 68 , 199 - 209 10.1016/j.ymben.2021.10.006
Salamanca, D., Bühler, K., Engesser, K.-H., Schmid, A., Karande, R. (2021):
Whole-cell biocatalysis using the Acidovorax sp. CHX100 Δ6HX for the production of ω-hydroxycarboxylic acids from cycloalkanes
New Biotech. 60 , 200 - 206 10.1016/j.nbt.2020.10.009
Welter, E.S., Kött, S., Brandenburg, F., Krömer, J.O., Goepel, M., Schmid, A., Gläser, R. (2021):
Figures of merit for photocatalysis: Comparison of NiO/La-NaTaO₃ and Synechocystis sp. PCC 6803 as a semiconductor and a bio-photocatalyst for water splitting
Catalysts 11 (11), art. 1415 10.3390/catal11111415

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2020 (3)

Mock, M., Schmid, A., Bühler, K. (2020):
Directed reaction engineering boosts succinate formation of Synechocystis sp. PCC 6803_Δsll1625
Biotechnol. J. 15 (11), art. 2000127 10.1002/biot.202000127
Schirmer, M., Wink, K., Ohla, S., Belder, D., Schmid, A., Dusny, C. (2020):
Conversion efficiencies of a few living microbial cells detected at a high throughput by droplet-based ESI-MS
Anal. Chem. 92 (15), 10700 - 10708 10.1021/acs.analchem.0c01839
Willrodt, C., Gröning, J.A.D., Nerke, P., Koch, R., Scholtissek, A., Heine, T., Schmid, A., Bühler, B., Tischler, D. (2020):
Highly efficient access to (S)-sulfoxides utilizing a promiscuous flavoprotein monooxygenase in a whole-cell biocatalyst format
ChemCatChem 12 (17), 4664 - 4671 10.1002/cctc.201901894

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2019 (12)

David, C., Schmid, A., Bühler, K. (2019):
Cellular physiology controls photoautotrophic production of 1,2‐propanediol from pools of CO₂ and glycogen
Biotechnol. Bioeng. 116 (4), 882 - 892 10.1002/bit.26883
Dusny, C., Lohse, M., Reemtsma, T., Schmid, A., Lechtenfeld, O.J. (2019):
Quantifying a biocatalytic product from a few living microbial cells using microfluidic cultivation coupled to FT-ICR-MS
Anal. Chem. 91 (11), 7012 - 7018 10.1021/acs.analchem.9b00978
Grund, M., Jakob, T., Wilhelm, C., Bühler, B., Schmid, A. (2019):
Electron balancing under different sink conditions reveals positive effects on photon efficiency and metabolic activity of Synechocystis sp. PCC 6803
Biotechnol. Biofuels 12 , art. 43 10.1186/s13068-019-1378-y
Halan, B., Tschörtner, J., Schmid, A. (2019):
Generating electric current by bioartificial photosynthesis
In: Harnisch, F., Holtmann, D. (eds.)
Bioelectrosynthesis
Adv. Biochem. Eng. Biotechnol. 167
Springer Nature, p. 361 - 393 10.1007/10_2017_44
Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Data on mixed trophies biofilm for continuous cyclohexane oxidation to cyclohexanol using Synechocystis sp. PCC 6803
Data Brief 25 , art. 104059 10.1016/j.dib.2019.104059
Heuschkel, I., Hoschek, A., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-trophies biofilm cultivation in capillary reactors
MethodsX 6 , 1822 - 1831 10.1016/j.mex.2019.07.021
Hoschek, A., Bühler, B., Schmid, A. (2019):
Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
Biotechnol. Bioeng. 116 (8), 1887 - 1900 10.1002/bit.27006
Hoschek, A., Heuschkel, I., Schmid, A., Bühler, B., Karande, R., Bühler, K. (2019):
Mixed-species biofilms for high-cell-density application of Synechocystis sp. PCC 6803 in capillary reactors for continuous cyclohexane oxidation to cyclohexanol
Bioresour. Technol. 282 , 171 - 178 10.1016/j.biortech.2019.02.093
Hoschek, A., Toepel, J., Hochkeppel, A., Karande, R., Bühler, B., Schmid, A. (2019):
Light‐dependent and aeration‐independent gram‐scale hydroxylation of cyclohexane to cyclohexanol by CYP450 harboring Synechocystis sp. PCC 6803
Biotechnol. J. 14 (8), art. 1800724 10.1002/biot.201800724
Mock, M., Schmid, A., Bühler, K. (2019):
Photoautotrophic production of succinate via the oxidative branch of the tricarboxylic acid cycle influences glycogen accumulation in Synechocystis sp. PCC 6803
Algal Res. 43 , art. 101645 10.1016/j.algal.2019.101645
Ütkür, F.Ö., Schmid, A., Bühler, B. (2019):
Anaerobic C-H oxyfunctionalization: Coupling of nitrate reduction and quinoline hydroxylation in recombinant Pseudomonas putida
Biotechnol. J. 14 (8), art. 1800615 10.1002/biot.201800615
Volmer, J., Lindmeyer, M., Seipp, J., Schmid, A., Bühler, B. (2019):
Constitutively solvent-tolerant Pseudomonas taiwanensis VLB120ΔCΔttgV supports particularly high styrene epoxidation activities when grown under glucose excess conditions
Biotechnol. Bioeng. 116 (5), 1089 - 1101 10.1002/bit.26924

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2018 (5)

David, C., Schmid, A., Adrian, L., Wilde, A., Bühler, K. (2018):
Production of 1,2-propanediol in photoautotrophic Synechocystis is linked to glycogen turn-over
Biotechnol. Bioeng. 115 (2), 300 - 311 10.1002/bit.26468
Hoschek, A., Schmid, A., Bühler, B. (2018):
In situ O₂ generation for biocatalytic oxyfunctionalization reactions
ChemCatChem 10 (23), 5366 - 5371 10.1002/cctc.201801262
Karande, R., Salamanca, D., Schmid, A., Buehler, K. (2018):
Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120
Biotechnol. Bioeng. 115 (2), 312 - 320 10.1002/bit.26469
Oehling, V., Klaassen, P., Frick, O., Dusny, C., Schmid, A. (2018):
L-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain
Biotechnol. Biofuels 11 , art. 231 10.1186/s13068-018-1231-8
Schaffer, S., Schmid, A. (2018):
Aminolauric acid: From design of a multistep synthesis route to large‐scale process development
Chem. Ing. Tech. 90 (9), 1334 - 1334 10.1002/cite.201855436

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2017 (11)

Fritzsch, F.S.O., Blank, L.M., Dusny, C., Schmid, A. (2017):
Miniaturized octupole cytometry for cell type independent trapping and analysis
Microfluid. Nanofluid. 21 (8), art. 130 10.1007/s10404-017-1969-5
Halan, B., Vassilev, I., Lang, K., Schmid, A., Buehler, K. (2017):
Growth of Pseudomonas taiwanensis VLB120ΔC biofilms in the presence of n-butanol
Microb. Biotechnol. 10 (4), 745 - 755 10.1111/1751-7915.12413
Hoschek, A., Bühler, B., Schmid, A. (2017):
Overcoming the gas-liquid mass transfer of oxygen by coupling photosynthetic water oxidation with biocatalytic oxyfunctionalization
Angew. Chem.-Int. Edit. 56 (47), 15146 - 15149 10.1002/anie.201706886
Kadisch, M., Julsing, M.K., Schrewe, M., Jehmlich, N., Scheer, B., von Bergen, M., Schmid, A., Bühler, B. (2017):
Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance
Biotechnol. Bioeng. 114 (4), 874 - 884 10.1002/bit.26213
Kadisch, M., Schmid, A., Bühler, B. (2017):
Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing
J. Ind. Microbiol. Biotechnol. 44 (3), 339 - 351 10.1007/s10295-016-1890-z
Kadisch, M., Willrodt, C., Hillen, M., Bühler, B., Schmid, A. (2017):
Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts
Biotechnol. J. 12 (8), art. 1600170 10.1002/biot.201600170
Rosenthal, K., Oehling, V., Dusny, C., Schmid, A. (2017):
Beyond the bulk: disclosing the life of single microbial cells
FEMS Microbiol. Rev. 41 (6), 751 - 780 10.1093/femsre/fux044
Schmutzler, K., Kupitz, K., Schmid, A., Buehler, K. (2017):
Hyperadherence of Pseudomonas taiwanensis VLB120ΔC increases productivity of (S)-styrene oxide formation
Microb. Biotechnol. 10 (4), 735 - 744 10.1111/1751-7915.12378
Theodosiou, E., Breisch, M., Julsing, M.K., Falcioni, F., Bühler, B., Schmid, A. (2017):
An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli
Biotechnol. Bioeng. 114 (7), 1511 - 1520 10.1002/bit.26281
Volmer, J., Schmid, A., Bühler, B. (2017):
The application of constitutively solvent-tolerant P. taiwanensis VLB120ΔCΔttgV for stereospecific epoxidation of toxic styrene alleviates carrier solvent use
Biotechnol. J. 12 (7), art. 1600558 10.1002/biot.201600558
Willrodt, C., Halan, B., Karthaus, L., Rehdorf, J., Julsing, M.K., Buehler, K., Schmid, A. (2017):
Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow
Biotechnol. Bioeng. 114 (2), 281 - 290 10.1002/bit.26071

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2016 (10)

Dusny, C., Schmid, A. (2016):
Neue Einblicke in die Welt isolierter Mikroben
Biospektrum 22 (7), 708 - 711 10.1007/s12268-016-0748-2
Dusny, C., Schmid, A. (2016):
The MOX promoter in Hansenula polymorpha is ultrasensitive to glucose-mediated carbon catabolite repression
FEMS Yeast Res. 16 (6), fow067 10.1093/femsyr/fow067
Halan, B., Karande, R., Buehler, K., Schmid, A. (2016):
Catalytic Pseudomonas taiwanensis VLB120ΔC biofilms thrive in a continuous pure styrene generated by multiphasic segmented flow in a capillary microreactor
J. Flow Chem. 6 (1), 39 - 42 10.1556/1846.2015.00037
Karande, R., Debor, L., Salamanca, D., Bogdahn, F., Engesser, K.-H., Buehler, K., Schmid, A. (2016):
Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms
Biotechnol. Bioeng. 113 (1), 52 - 61 10.1002/bit.25696
Karande, R., Schmid, A., Buehler, K. (2016):
Applications of multiphasic microreactors for biocatalytic reactions
Org. Process Res. Dev. 20 (2), 361 - 370 10.1021/acs.oprd.5b00352
Ladkau, N., Assmann, M., Schrewe, M., Julsing, M.K., Schmid, A., Bühler, B. (2016):
Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli
Metab. Eng. 36 , 1 - 9 10.1016/j.ymben.2016.02.011
Lange, K., Schmid, A., Julsing, M.K. (2016):
Δ9-Tetrahydrocannabinolic acid synthase: The application of a plant secondary metabolite enzyme in biocatalytic chemical synthesis
J. Biotechnol. 233 , 42 - 48 10.1016/j.jbiotec.2016.06.022
Schmutzler, K., Kracht, O.N., Schmid, A., Buehler, K. (2016):
Trophic regulation of autoaggregation in Pseudomonas taiwanensis VLB120
Appl. Microbiol. Biotechnol. 100 (1), 347 - 360 10.1007/s00253-015-7006-2
Sudarsan, S., Blank, L.M., Dietrich, A., Vielhauer, O., Takors, R., Schmid, A., Reuss, M. (2016):
Dynamics of benzoate metabolism in Pseudomonas putida KT2440
Metab. Eng. Commun. 3 , 97 - 110 10.1016/j.meteno.2016.03.005
Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K. (2016):
Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production
Biotechnol. Bioeng. 113 (6), 1305 - 1314 10.1002/bit.25883

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2015 (17)

Collins, J., Grund, M., Brandenbusch, C., Sadowski, G., Schmid, A., Bühler, B. (2015):
The dynamic influence of cells on the formation of stable emulsions in organic–aqueous biotransformations
J. Ind. Microbiol. Biotechnol. 42 (7), 1011 - 1026 10.1007/s10295-015-1621-x
David, C., Bühler, K., Schmid, A. (2015):
Stabilization of single species Synechocystis biofilms by cultivation under segmented flow
J. Ind. Microbiol. Biotechnol. 42 (7), 1083 - 1089 10.1007/s10295-015-1626-5
Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., Schmid, A. (2015):
Technical bias of microcultivation environments on single-cell physiology
Lab Chip 15 (8), 1822 - 1834 10.1039/c4lc01270d
Dusny, C., Schmid, A. (2015):
Challenging biological limits with microfluidic single cell analysis
Microb. Biotechnol. 8 (1), 23 - 25 10.1111/1751-7915.12252
Dusny, C., Schmid, A. (2015):
Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes
Environ. Microbiol. 17 (6), 1839 - 1856 10.1111/1462-2920.12667
Köhler, K.A.K., Rühl, J., Blank, L.M., Schmid, A. (2015):
Integration of biocatalyst and process engineering for sustainable and efficient n-butanol production
Eng. Life Sci. 15 (1), 4 - 19 10.1002/elsc.201400041
Lang, K., Buehler, K., Schmid, A. (2015):
Multistep synthesis of (S)-3-hydroxyisobutyric acid from glucose using Pseudomonas taiwanensis VLB120 B83 T7 catalytic biofilms
Adv. Synth. Catal. 357 (8), 1919 - 1927 10.1002/adsc.201500205
Lange, K., Schmid, A., Julsing, M.K. (2015):
Δ⁹-Tetrahydrocannabinolic acid synthase production in Pichia pastoris enables chemical synthesis of cannabinoids
J. Biotechnol. 211 , 68 - 76 10.1016/j.jbiotec.2015.06.425
Lindmeyer, M., Jahn, M., Vorpahl, C., Müller, S., Schmid, A., Bühler, B. (2015):
Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains
Front. Microbiol. 6 , art. 1042 10.3389/fmicb.2015.01042
Lindmeyer, M., Meyer, D., Kuhn, D., Bühler, B., Schmid, A. (2015):
Making variability less variable: matching expression system and host for oxygenase-based biotransformations
J. Ind. Microbiol. Biotechnol. 42 (6), 851 - 866 10.1007/s10295-015-1615-8
Rosenthal, K., Falke, F., Frick, O., Dusny, C., Schmid, A. (2015):
An inert continuous microreactor for the isolation and analysis of a single microbial cell
Micromachines 6 (12), 1836 - 1855 10.3390/mi6121459
Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015):
Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897 10.1007/s00253-015-6599-9
Schmutzler, K., Schmid, A., Buehler, K. (2015):
A three-step method for analysing bacterial biofilm formation under continuous medium flow
Appl. Microbiol. Biotechnol. 99 (14), 6035 - 6047 10.1007/s00253-015-6628-8
Theodosiou, E., Frick, O., Bühler, B., Schmid, A. (2015):
Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation
Microb. Cell. Fact. 14 , art. 108 10.1186/s12934-015-0298-1
Volmer, J., Schmid, A., Bühler, B. (2015):
Guiding bioprocess design by microbial ecology
Curr. Opin. Microbiol. 25 , 25 - 32 10.1016/j.mib.2015.02.002
Willrodt, C., Hoschek, A., Bühler, B., Schmid, A., Julsing, M.K. (2015):
Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation
Biotechnol. Bioeng. 112 (9), 1738 - 1750 10.1002/bit.25592
Willrodt, C., Karande, R., Schmid, A., Julsing, M.K. (2015):
Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants
Curr. Opin. Biotechnol. 35 , 52 - 62 10.1016/j.copbio.2015.03.010

to index

Publications without UFZ-affiliation

2015

Köhler K. A. K., Blank L. M., Frick O. and Schmid A. (2015)
D-Xylose assimilation via the Weimberg pathway by solvent tolerant Pseudomonas taiwanensis VLB120.
Environmental Microbiology, 17(1):156-170

Looße C., Galozzi S., Debor L., Julsing M. K., Bühler B., Schmid A., Barkovits K., Müller T. and Marcus K. (2015)
Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples.
EuPA Open Proteomics, 7:20-26

Falcioni F., Bühler B. and Schmid A. (2015)
Efficient hydroxyproline production from glucose in minimal media by Corynebacterium glutamicum.
Biotechnology and Bioengineering, 112(2):322-330

Brandenbusch C., Collins J., Glonke S., Hoffrogge R., Grunwald K., Bühler B., Schmid A. and Sadowski G. (2015)
Process boundaries of irreversible scCO2-assisted phase separation in biphasic whole-cell biocatalysis.
Biotechnology and Bioengineering, 112(11):2316-2323

2014

Tomaszewski B., Schmid A. and Bühler K. (2014)
Biocatalytic production of catechols using a high pressure tube-in-tube segmented flow microreactor.
Organic Process Research & Development, 18(11):1516–1526
Ruinatscha R., Bühler K. and Schmid A. (2014)
Development of a high performance electrochemical cofactor regeneration modul and its application for the continuos reduction of FAD.
Journal of Molecular Catalysis B, 103:100–105

Volmer J., Neumann C., Bühler B. and Schmid A. (2014)
Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity.
Applied and Environmental Microbiology, 80(20):6539-6548

Willrodt C., David C., Cornelissen S., Bühler B., Julsing M. K. and Schmid A. (2014)
Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.
Biotechnology Journal, 9(8):1000–1012

Churakova E.*, Tomaszewski B.*, Bühler K., Schmid A., Arends I. W. C. E and Hollmann F. (2014)
Hydrophobic formic acid esters for cofactor regeneration in aqueous / organic two-liquid phase systems.
Topics in Catalysis, 57(5):385-391
* These authors contributed equally to this work.

Lang K., Zierow J., Bühler K. and Schmid A. (2014)
Metabolic engineering of Pseudomonas sp. VLB120 towards a platform biocatalyst for the production of isobutyric acid and other secondary metabolites.
Microbial Cell Factories, 13:2

Schrewe M., Julsing M. K., Lange K., Czarnotta E, Schmid A. and Bühler B. (2014)
Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.
Biotechnology and Bioengineering, 111(9):1820-1830

Tomaszewski B., Lloyd R., Warr A., Bühler K. and Schmid A. (2014)
Regioselective biocatalytic aromatic hydroxylation in a gas-liquid multiphase tube-in-tube reactor.
ChemCatChem, 6(9):2567–2576

Halan B., Letzel T., Schmid A. and Bühler K. (2014)
Scalability of a solid support membrane aerated biofilm reactor for the continuous synthesis of (S)-styrene oxide.
Biotechnology Journal, 9(10):1339-1349

Karande R.*, Halan B.*, Schmid A. and Bühler K. (2014)
Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.
Biotechnology and Bioengineering, 111(9):1831-1840
* These authors contributed equally to this work.

Sudarsan S., Dethlefsen S., Blank L. M., Siemann-Herzberg M. and Schmid A. (2014)
The functional structure of central carbon metabolism.
Applied and Environmental Microbiology, 80(17):5292-5303

Ladkau N., Schmid A. and Bühler B. (2014)
The microbial cell - functional unit for energy-dependent multistep biocatalysis.
Current Opinion in Biotechnology, 30:178-189

2013

Köhler K. A. K., Rückert C., Schatschneider S., Vorhölter F., Sczepanowski R., Blank L. M., Niehaus K., Goesmann A., Pühler A., Kalinowski J. and Schmid A. (2013)
Complete genome sequence of Pseudomonas sp. strain VLB120 a solvent tolerant, styrene degrading bacterium, isolated from forest soil.
Journal of Biotechnology, 168(4):729-730

Schrewe M., Ladkau N., Bühler B. and Schmid A. (2013)
Direct terminal alkylamino-functionalization via multistep biocatalysis in one recombinant whole-cell catalyst.
Advanced Synthesis and Catalysis, 355(9):1693-1697

Gross R., Bühler K. and Schmid A. (2013)
Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide.
Biotechnology and Bioengineering, 110(2):424–436

Fritzsch F. S. O., Rosenthal K., Kampert A., Howitz S., Dusny C., Blank L. M. and Schmid A. (2013)
Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments.Lab on a Chip, 13(3):397-408

Falcioni F., Blank L. M., Frick O., Karau A., Bühler B. and Schmid A. (2013)
Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.
Applied and Environmental Microbiology, 79(9):3091-3100

Jahn M., Seifert J., von Bergen M., Schmid A., Bühler B. and Müller S. (2013)
Subpopulation-proteomics in prokaryotic populations.
Current Opinion in Biotechnology, 24(1):79-87

Kuhn D., Fritzsch F. S. O., Zhang X., Wendisch V. F., Blank L. M., Bühler B. and Schmid A. (2013)
Subtoxic product levels limit the epoxidation capacity of recombinant E. coli by increasing microbial energy demands.
Journal of Biotechnology, 163(2):194-203

Schrewe M.*, Julsing M. K.*, Bühler B. and Schmid A. (2013)
Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.
Chemical Society Reviews, 42(15):6346 - 6377
* These authors contributed equally to this work.

Cornelissen S., Julsing M. K., Volmer J., Riechert O., Schmid A. and Bühler B. (2013)
Whole-cell-based CYP153A6-catalyzed (S)-limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL.
Biotechnology and Bioengineering, 110(5):1282-1292

2012

Halan B., Bühler K. and Schmid A. (2012)
Biofilms as living catalysts in chemical synthesis.
Trends in Biotechnology, 30(9):453-465

Cornelissen S., Julsing M. K., Schmid A. and Bühler B. (2012)
Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis.
Journal of Industrial Microbiology and Biotechnology, 39(2):275-287

Ütkür F.Ö., Tran T.-T., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
Integrating organic-aqueous biocatalysis and product recovery using quinaldine 4-oxidase in living P. putida KT2440 (pKP1).
Journal of Industrial Microbiology & Biotechnology, 39(7):1049-1059

Dusny C., Fritzsch F. S. O., Frick O. and Schmid A. (2012)
Isolated Microbial Single Cells and Resulting Micropopulations Grow Faster in Controlled Environments.
Applied and Environmental Microbiology, 78(19):7132-7136

Gernaey K. V., Baganz F., Franco-Lara E., Kensy F., Krühne U., Luebberstedt M., Marx U., Palmqvist E., Schmid A., Schubert F. and Mandenius C.-F. (2012)
Monitoring and control of microbioreactors for applications in biotechnology: A review.
Biotechnology Journal, 7:1308-1314, DOI-Link

Kuhn D., Bühler B. and Schmid A. (2012)
Production host selection for asymmetric styrene epoxidation: Escherichia coli vs. solvent tolerant Pseudomonas.
Journal of Industrial Microbiology and Biotechnology, 39(8):1125-1133

Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
Biotechnology and Bioengineering, 109(5):1109-1119

Blank L. M., Deshpande R., Schmid A. and Hayen H. (2012)
Simultaneous analysis of carbon and nitrogen metabolism by ultra-high-resolution mass spectrometry applying 13C- and 15N-labeled substrates simultaneously.
Analytical and Bioanalytical Chemistry, 403(8):2291-2305

Fritzsch F. S. O.*, Dusny C.*, Frick O. and Schmid A. (2012)
Single Cell Analysis in Biotechnology, Systems Biology, and Biocatalysis.
Annual Review of Chemical and Biomolecular Engineering, 3:129-155
* These authors contributed equally to this work.

Braun A., Geier M., Bühler B., Schmid A., Mauersberger S. and Glieder A. (2012)
Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.
Microbial Cell Factories, 11:106

Rühl J., Hein E. M., Hayen H., Schmid A. and Blank L. M. (2012)
The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition related alterations.
Microbial Biotechnology, 5(1):45-58

Julsing M. K.*, Schrewe M.*, Cornelissen S., Hermann I., Schmid A. and Bühler B. (2012)
The outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in E. coli.
Applied and Environmental Microbiology, 78(16):5724-5733
* These authors contributed equally to this work.

2011

Heyland J., Fu J., Blank L. M. and Schmid A. (2011)
Carbon metabolism limits recombinant protein production in Pichia pastoris.
Biotechnology and Bioengineering, 108(8):1942-1953

Cornelissen S., Liu S, Deshmukh A.T., Schmid A. and Bühler B. (2011)
Cell physiology can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.
Journal of Industrial Microbiology and Biotechnology, 38:1359-1370

Ladkau N., Hermann I., Bühler B. and Schmid A. (2011)
Enzyme catalyzed laurolactam synthesis via intramolecular amide bond formation in aqueous solution.
Advanced Synthesis and Catalysis, 353(13):2501-2510

Ruinatscha R., Karande R., Bühler K. and Schmid A. (2011)
Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using Sepabead EC-EA and EC-Q1A anion-exchange carriers.
Molecules, 16(7):5975-5988

Schrewe M., Magnusson A., Willrodt C., Bühler B. and Schmid A. (2011)
Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis.
Advanced Synthesis and Catalysis, 353:3485 – 3495

Karande R., Schmid A. and Bühler K. (2011)
Miniaturizing Biocatalysis: Enzyme catalyzed reactions in an aqueous/organic segmented flow capillary microreactor.
Advanced Synthesis and Catalysis, 353(13):2511-2521

Fritzsch F. S. O., Kortmann H., Lonzynski J., Blank L. M. and Schmid A. (2011)
Pressure resistant and reversible on-tube-sealing for microfluidics.
Microfluidics and Nanofluidics, 10(3):679-684

Heyland J., Blank L. M. and Schmid A. (2011)
Quantification of metabolic limitations during recombinant protein production in Escherichia coli.
Journal of Biotechnology, 155(2):178-184

Halan B., Schmid A. and Bühler K. (2011)
Real time solvent tolerance analysis of Pseudomonas sp. strain VLB120ΔC catalytic biofilms.
Applied and Enviromental Microbiology, 77(5):1563-1571

Ütkür F.Ö., Gaykawad S., Bühler B. and Schmid A. (2011)
Regioselective aromatic hydroxylation of quinaldine with water using quinaldine 4-oxidase in recombinant Pseudomonas putida.
Journal of Industrial Microbiology and Biotechnology, 38(8):1067-1077

Ebert B. E., Kurth F., Grund M., Blank L. M. and Schmid A. (2011)
Response of P. putida KT2440 to increased NADH and ATP demand.
Applied and Environmental Microbiology, 77(18):6597–6605

Kortmann H., Blank L. M. and Schmid A. (2011)
Single cell analytics: an overview.
Advances in Biochemical Engineering and Biotechnology, 124:99-122

2010

Gross R., Lang K., Bühler K. and Schmid A. (2010)
Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis.
Biotechnology and Bioengineering, 105(4):705-717

Schmid A., Kortmann H., Dittrich P. and Blank L. M. (2010)
Chemical and biological single cell analysis.
Current Opinion in Biotechnology, 21(1):12-20

Brandenbusch C., Bühler B., Hoffmann P., Sadowski G. and Schmid A. (2010)
Efficient phase separation and product recovery in organic-aqueous bioprocessing using supercritical carbon dioxide.
Biotechnology and Bioengineering, 107(4):642-651

Karande R., Schmid A. and Bühler K. (2010)
Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity.
Langmuir, 26(11):9152-9159

Gardossi L, Poulsen P. B., Ballesteros A., Hult K., Švedas V. K., Vasić-Rački Ð., Carrea G., Magnusson A., Schmid A., Wohlgemuth R. and Halling P. J. (2010)
Guidelines for reporting of biocatalytic reactions.
Trends in Biotechnology, 28(4):171-180

Kuhn D., Kholiq A., Heinzle E., Bühler B. and Schmid A. (2010)
Intensification and economic and ecological assessment of a biocatalytic oxyfunctionalization process.
Green Chemistry, 12(5):815-827

Heck T., Makam S., Lutz J., Blank L. M., Schmid A., Seebach Dieter, Kohler H.-P. E. and Geueke B. (2010)
Kinetic analysis of L-carnosine formation by beta-aminopeptidases.
Advanced Synthesis and Catalysis, 352(2-3):407-415

Halan B., Schmid A. and Bühler K. (2010)
Maximizing the productivity of catalytic biofilms on solid supports in membrane-aerated reactors.
Biotechnology and Bioengineering, 106(4):516-527

Holm A. K., Blank L. M., Oldiges M., Schmid A., Solem C., Jensen P. R. and Vemuri G. N. (2010)
Metabolic and transcriptional response to cofactor perturbations in Escherichia coli.
The Journal of Biological Chemistry, 285(23):17498-17506

Heyland J., Fu J., Blank L. M. and Schmid A. (2010)
Quantitative physiology of Pichia pastoris during glucose-limited high cell-density fed-batch cultivation for recombinant protein production.
Biotechnology and Bioengineering, 107(2):357-368

Heyland J., Antweiler N., Lutz J., Heck T., Geueke B., Kohler H.-P. E., Blank L. M. and Schmid A. (2010)
Simple enzymatic procedure for carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recycling.
Microbial Biotechnology, 3(1):74-83

Schmid A. and Blank L. M. (2010)
Systems biology: Hypothesis-driven omics integration.
Nature Chemical Biology (News & Views), 6:485-487

Kuhn D., Blank L. M., Schmid A. and Bühler B. (2010)
Systems biotechnology - rational biocatalyst and bioprocess design.
Engineering in Life Sciences, 10(5):384–397

2009

Kortmann H., Blank L. M. and Schmid A. (2009)
A rapid, reliable, and automatable interface for microfluidic devices.
Lab-on-a-Chip, 9:1455 - 1460

Maddula Sasidhar, Blank L. M., Schmid A. and Baumbach J. I. (2009)
Detection of volatile metabolites of Escherichia coli by multi capillary column coupled ion mobility spectrometry.
Analytical and Bioanalytical Chemistry, 394(3):791-800

Hein E. M., Blank L. M., Heyland J., Baumbach J. I., Schmid A. and Hayen H. (2009)
Glycerophospholipid profiling by HPLC/MS using exact mass measurements and MSn fragmentation experiments in parallel.
Rapid Communications in Mass Spectrometry, 23(11):1636-1646

Wierckx N. J. P., Ruijssenaars H. J., de Winde J. H., Schmid A. and Blank L. M. (2009)
Metabolic flux analysis of a phenol producing mutant of Pseudomonas putida S12: verification and complementation of hypotheses derived from transcriptomics.
Journal of Biotechnology, 143(2):124–129

Rosche B., Li X. Z., Schmid A. and Bühler K. (2009)
Microbial biofilms: A concept for industrial catalysis?
TRENDS in Biotechnology, 27(11):636-643

Ruinatscha R., Dusny C., Bühler K. and Schmid A. (2009)
Productive asymmetric styrene epoxidation based on a next generation electroenzymatic methodology.
Advanced Synthesis and Catalysis, 351:2505-2515

Rühl J., Schmid A. and Blank L. M. (2009)
Selected Pseudomonas putida strains able to grow in high concentrations of butanol.
Applied and Environmental Microbiology, 75(13):4653-4656

Kortmann H., Blank L. M. and Schmid A. (2009)
Single cell analysis reveals unexpected growth phenotype of S. cerevisiae.
Cytometry: Part A, 75A(2):130-139

Kortmann H., Chasanis P., Blank L. M., Franzke J., Kenig E. Y. and Schmid A. (2009)
The Envirostat - A new bioreactor concept.
Lab-on-a-Chip, 9:576–585

Kortmann H., Kurth F., Blank L. M., Dittrich P. and Schmid A. (2009)
Towards real time analysis of protein secretion from single cells.
Lab-on-a-Chip, 9:3047-3049

2008

Tee K. L., Dmytrenko O., Otto K., Schmid A. and Schwaneberg U. (2008)
A p-nitrothiophenolate screening system for the directed evolution of a two-component epoxygenase (StyAB).
Journal of Molecular Catalysis B: Enzymatic, 50(2-4):121-127

Kurth F., Schumann C. A., Blank L. M., Schmid A., Manz A. and Dittrich P. (2008)
A bilayer microfluidic chip for diffusion-controlled activation of yeast species.
Journal of Chromatography A, 1206(1):77-82

Julsing M. K., Cornelissen S., Bühler B. and Schmid A. (2008)
Heme-iron oxygenases: powerful industrial biocatalysts?
Current Opinion in Chemical Biology, 12(2):177-186

Blank L. M., Ebert B. E., Bühler B. and Schmid A. (2008)
Metabolic capacity estimation of Escherichia coli as platform for redox biocatalysis: Constraint based modeling and experimental verification.
Biotechnology and Bioengineering, 100(6):1050-1065

Blank L. M., Ionidis G., Ebert B. E., Bühler B. and Schmid A. (2008)
Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.
FEBS Journal, 275(20):5173-5190

Bühler B., Park J. B., Blank L. M. and Schmid A. (2008)
NADH availability limits asymmetric biocatalytic epoxidation in growing recombinant Escherichia coli.
Applied and Environmental Microbiology, 74(5):1436-1446

Höllrigl V., Hollmann F., Kleeb A., Bühler K. and Schmid A. (2008)
TADH, the thermostable alcohol dehydrogenase from Thermus sp. ATN1: A versatile new biocatalyst for organic synthesis.
Applied Microbiology and Biotechnology, 81(2):263-273

Hollmann F. and Schmid A. (2008)
Towards [Cp*Rh(bpy)(H2O)2+-promoted P450 catalysis: Direct regeneration of CytC.
Journal of Inorganic Biochemistry, Epub

2007

Park J. B., Bühler B., Panke S., Witholt B. and Schmid A. (2007)
Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent tolerant Pseudomonas sp. strain VLB120ΔC.
Biotechnology and Bioengineering, 98(6):1219-1229

Kortmann H., Blank L. M. and Schmid A. (2007)
Cultivation of dielectrophoretically trapped single cells under optimal environmental conditions.
Conference paper µTAS 2007, Conference Proceedings, (07.10.-11.10.2007):715-717

Höllrigl V., Otto K. and Schmid A. (2007)
Electroenzymatic asymmetric reduction of rac-3-methylcyclohexanone to (1S,3S)-3-methylcyclohexanol in organic/aqueous media catalyzed by a thermophilic alcohol dehydrogenase.
Advanced Synthesis & Catalysis, 349(8-9):1337-1340

Gross R., Hauer B., Otto K. and Schmid A. (2007)
Microbial biofilms: New catalysts for maximising productivty of long-term biotransformations.
Biotechnology and Bioengineering, 98(6):1123-1134

2006

Bühler B., Straathof A. J. J., Witholt B. and Schmid A. (2006)
Analysis of two-liquid-phase multistep biooxidation based on a process model: Indications for biological energy shortage.
Organic Process Research and Development, 10(3):628-643

Feenstra K. A., Hofstetter K., Bosch R., Schmid A., Commandeur J. M. N. and Vermeulen N. P. E. (2006)
Enantioselective substrate binding in a monooxygenase protein model by molecular dynamics and docking.
Biophysical Journal, 91(9):3026-3216

Hollmann F., Hofstetter K. and Schmid A. (2006)
Non-enzymatic regeneration of nicotinamide and flavin cofactors for oxygenase catalysis.
Trends in Biotechnology, 24(4):163-171

Meyer D., Bühler B. and Schmid A. (2006)
Process and catalyst design objectives for specific redox biocatalysis.
Advances in Applied Microbiology, 59:53-91

Ruinatscha R.*, Höllrigl V.*, Otto K. and Schmid A. (2006)
Productivity of selective electroenzymatic reduction and oxidation reactions: Theoretical and practical considerations.
Advanced Synthesis & Catalysis, 348(15):2015-2026
* These authors contributed equally to this work.

Yildrim S., Ruinatscha R., Gross R., Wohlgemuth R., Kohler H.-P. E., Witholt B. and Schmid A. (2006)
Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles.
Journal of Molecular Catalysis B-Enzymatic, 38(2):76-83

Park J. B.*, Bühler B.*, Habicher T., Hauer B., Panke S., Witholt B. and Schmid A. (2006)
The efficiency of recombinant Escherichia coli as biocatalyst for stereospecific epoxidation.
Biotechnology and Bioengineering, 95(3):501-512
* These authors contributed equally to this work.

2005

Meyer D., Witholt B. and Schmid A. (2005)
Suitability of recombinant Escherichia coli and Pseudomonas putida for selective biotransformation of m-nitrotoluene by xylene monooxygenase.
Applied and Environmental Microbiology, 71(11):6624-6632

Hollmann F., Kleeb A., Otto K. and Schmid A. (2005)
Coupled chemoenzymatic transfer hydrogenation catalysis for enantioselective reduction and oxidation reactions
(Corrigendum in Tetrahedron-Asymmetry (2006), 17:867-868).
Tetrahedron-Asymmetry, 16(21):3512-3519

Hollmann F., Hofstetter K., Habicher T., Hauer B. and Schmid A. (2005)
Direct electrochemical regeneration of monooxygenase subunits for biocatalytic asymmetric epoxidation.
Journal of the American Chemical Society, 127(18):6540-6541

Unversucht S., Hollmann F., Schmid A. and van Pée K.-H. (2005)
FADH2-dependence of tryptophan 7-halogenase.
Advanced Synthesis & Catalysis, 347(7-8):1163-1167

Neumann G., Kabelitz N., Zehnsdorf A., Miltner A., Lippold H., Meyer D., Schmid A. and Heipieper H. J. (2005)
Prediction of the adaptability of Pseudomonas putida DOT-T1E to a second phase of a solvent for economically sound two-phase biotransformations.
Applied and Environmental Microbiology, 71(11):6606-6612

Yildrim S., Franko T. T., Wohlgemuth R., Kohler H.-P. E., Witholt B. and Schmid A. (2005)
Recombinant chlorobenzene dioxygenase from Pseudomonas sp. P51: A biocatalyst for specific oxidation of aromatic nitriles.
Advanced Synthesis & Catalysis, 347(7-8):1060-1072

2004

Yildrim S., Zezula J., Hudlicky T., Witholt B. and Schmid A. (2004)
Asymmetric dihydroxylation of cinnamonitrile to trans-3-[(5S,6R)-5,6-dihydroxy- cyclohexa-1,3-dienyl]-acrylonitrile using chlorobenzene dioxygenase in Escherichia coli (pTEZ30).
Advanced Synthesis & Catalysis, 346(8):933-942

Otto K., Hofstetter K., Röthlisberger M., Witholt B. and Schmid A. (2004)
Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component non-heme flavin-diffusible styrene monooxigenase.
Journal of Bacteriology, 186(16):5292-5302

Lutz J., Hollmann F., Ho T.-V., Schnyder A., Fish R. H. and Schmid A. (2004)
Bioorganometallic chemistry: Biocatalytic oxidation reactions with biomimetic NAD+/NADH co-factors and [Cp*Rh(bpy)H]+ for selective organic synthesis.
Journal of Organometallic Chemistry, 689(25):4783-4790

Rojas A., Duque E., Schmid A., Hurtado A., Ramos J. L. and Segura A. (2004)
Biotransformation in double-phase systems: Physiological responses of Pseudomonas putida DOT-T1E to a double phase made of aliphatic alcohols and biosynthesis of substituted catechols.
Applied and Environmental Microbiology, 70(6):3637-3643

Hofstetter K., Lutz J., Lang I., Witholt B. and Schmid A. (2004)
Coupling biocatalytic asymmetric epoxidation and NADH regeneration in organic-aqueous emulsions.
Angewandte Chemie International Edition, 43(16):2163-2166

Hollmann F. and Schmid A. (2004)
Electrochemical regeneration of oxidoreductases for cell-free biocatalytic redox reactions.
Biocatalysis and Biotransformation, 22(2):63-88

Bühler B. and Schmid A. (2004)
Process implementation aspects for biocatalytic hydrocarbon oxyfunctionalisation.
Journal of Biotechnology, 113(1-3):183-210

2003

Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
Chemical biotechnology for the specific oxyfunctionalization of hydrocarbons an a technical scale.
Biotechnology and Bioengineering, 82(7):833-842

Meyer A., Tanner D., Schmid A., Sargent D. F., Kohler H.-P. E. and Witholt B. (2003)
Crystallization and preliminary X-ray analysis of native selenomethionine 2-hydroxybiphenyl 3-monooxygenase.
Acta Crystallographica Section D - Biological Crystallography, 59(4):741-743

van Beilen J. B., Duetz W. A., Schmid A. and Witholt B. (2003)
Issues relevant for the practical application of oxygenases.
Trends in Biotechnology, 21(4):170-177

Hollmann F., Lin P.-C., Witholt B. and Schmid A. (2003)
Stereospecific biocatalytic epoxidation: The first example of direct regeneration of a FAD-dependent monooxygenase for catalysis.
Journal of the American Chemical Society, 125(27):8209-8217

Meyer A., Held M., Schmid A., Kohler H.-P. E. and Witholt B. (2003)
Synthesis of 3-tert-butylcatechol by an engineered monooxygenase.
Biotechnology and Bioengineering, 81(5):518-524

Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
Use of the two-liquid phase concept to exploit kinetically controlled multistep biocatalysis.
Biotechnology and Bioengineering, 81(6):683-694

2002

Parales R. E., Bruce N. C., Schmid A. and Wackett L. P. (2002)
Biodegradation, biotransformation and biocatalysis (b3).
Applied and Environmental Microbiology, 68(10):4699-4709

Meyer A., Schmid A., Held M., Westphal A. H., Röthlisberger M., Kohler H.-P. E., van Berkel W. J. H. and Witholt B. (2002)
Changing the substrate reactivity of 2-hydroxybiphenyl 3-monooxygenase from Pseudomonas azelaica HBP1 by directed evolution.
Journal of Biological Chemistry, 277(7):5575-5582

Bühler B., Witholt B., Hauer B. and Schmid A. (2002)
Characterization and application of xylene monooxygenase for multistep biocatalysis.
Applied and Environmental Microbiology, 68(2):560-568

Meyer A., Würsten M., Schmid A., Kohler H.-P. E. and Witholt B. (2002)
Hydroxylation of indole by laboratory-evolved 2-hydroxybiphenyl 3-monooxygenase.
Journal of Biological Chemistry, 277(37):34161-34167

Li Z., van Beilen J. B., Duetz W. A., Schmid A., de Raadt A., Griengl H. and Witholt B. (2002)
Oxidative biotransformations using oxygenases.
Current Opinion in Chemical Biology, 6:136-144

Panke S., Held M., Wubbolts M. G., Witholt B. and Schmid A. (2002)
Pilot-scale production of S-styrene oxide from styrene by recombinant Escherichia coli synthesizing styrene monooxygenase.
Biotechnology and Bioengineering, 80(1):33-41

Lutz J., Mozhaev V. V., Khmelnitsky Y. L., Witholt B. and Schmid A. (2002)
Preparative application of 2-hydroxybiphenyl 3-monooxygenase with enzymatic cofactor regeneration in organic-aqueous reaction media.
Journal of Molecular Catalysis B-Enzymatic, 19-20:177-187

Straathof A. J. J., Panke S. and Schmid A. (2002)
The production of fine chemicals by biotransformations.
Current Opinion in Biotechnology, 13(6):548-556

Schmid A., Hollmann F., Park J. B. and Bühler B. (2002)
The use of enzymes in the chemical industry in Europe.
Current Opinion in Biotechnology, 13(4):359-366

Hollmann F., Witholt B. and Schmid A. (2002)
[Cp*Rh(bpy)(H2O)]2+: A versatile tool for efficient and non enzymatic regeneration of nicotinamide and flavin coenzymes.
Journal of Molecular Catalysis B-Enzymatic, 19-20:167-176

2001

Schmid A., Dordick J. S., Hauer B., Kiener A., Wubbolts M. G. and Witholt B. (2001)
Industrial biocatalysis today and tomorrow.
Nature, 409(6817):258-268

Schmid A., Hofstetter K., Feiten H.-J., Hollmann F. and Witholt B. (2001)
Integrated biocatalytic synthesis on gram scale: The highly enantioselective preparation of chiral oxiranes with styrene monooxygenase.
Advanced Synthesis & Catalysis, 343(6-7):732-737

Schmid A., Vereyken I., Held M. and Witholt B. (2001)
Preparative regio- and chemoselective functionalization of hydrocarbons catalyzed by cell free preparations of 2-hydroxybiphenyl 3-monooxygenase.
Journal of Molecular Catalysis B-Enzymatic, 11(4):455-462

Hollmann F., Schmid A. and Steckhan E. (2001)
The first synthetic application of a monooxygenase employing indirect electrochemical NADH regeneration.
Angewandte Chemie - International Edition in English, 40(1):169-171

Jaspers M. C. M., Schmid A., Sturme M. J. H., Goslings D. A. M., Kohler H.-P. E. and van der Meer J. R. (2001)
Transcriptional organization and dynamic expression of the hbpCAD genes, which encode the first three enzymes for 2-hydroxybiphenyl degradation in Pseudomonas azelaica HBP1.
Journal of Bacteriology, 183(1):270-279

2000

Jaspers M. C. M., Suske W. A., Schmid A., Goslings D. A. M., Kohler H.-P. E. and van der Meer J. R. (2000)
HbpR, a new member of the XylR/DmpR subclass within the NtrC family of bacterial transcriptional activators, regulates the expression of 2-hydroxybiphenyl metabolism in Pseudomonas azelaica.
Journal of Bacteriology, 182(2):405-417

Panke S., Wubbolts M. G., Schmid A. and Witholt B. (2000)
Production of enantiopure styrene oxide by recombinant Escherichia coli synthesizing a two-component styrene monooxygenase.
Biotechnology and Bioengineering, 69(1):91-100

Bühler B., Schmid A., Hauer B. and Witholt B. (2000)
Xylene monooxygenase catalizes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101.
Journal of Biological Chemistry, 275(14):10085-100092

1999

Held M., Schmid A., Kohler H.-P. E., Suske W. A., Witholt B. and Wubbolts M. G. (1999)
An integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst.
Biotechnology and Bioengineering, 62(6):641-648

Held M., Panke S., Kohler H.-P. E., Feiten H.-J., Schmid A., Wubbolts M. G. and Witholt B. (1999)
Solid phase extraction for biocatalytic production of toxic compounds.

1998

Schmid A., Kohler H.-P. E. and Engesser K.-H. (1998)
Escherichia coli JM101 pHBP461, a recombinant biocatalyst for the regioselective monohydroxylation of ortho-substituted phenols to their corresponding 3-substituted catechols.
Journal of Molecular Catalysis B-Enzymatic, 5(1-4):311-316

Held M., Suske W. A., Schmid A., Engesser K.-H., Kohler H.-P. E., Witholt B. and Wubbolts M. G. (1998)
Preparative scale production of 3-substituted catechols using a novel monooxygenase from Pseudomonas azelaica.
Journal of Molecular Catalysis B-Enzymatic, 5(1-4):87-93

Panke S., Witholt B., Schmid A. and Wubbolts M. G. (1998)
Towards a biocatalyst for S-styrene oxide production: characterization of the styrene degradation pathway of Pseudomonas sp. VLB120.
Applied and Environmental Microbiology, 64(6):2032-2043

1997

Schmid A., Rothe B., Altenbucher J., Ludwig W. and Engesser K.-H. (1997)
Characterisation of three distinct extradiol dioxygenases involved in mineralization of dibenzofuran by Terrabacter sp. strain DPO360.
Journal of Bacteriology, 179(1):53-62

Suske W. A., Held M., Schmid A., Fleischmann T., Wubbolts M. G. and Kohler H.-P. E. (1997)
Purification and characterization of 2-hydroxybiphenyl 3-monooxygenase, a novel NADH-dependent, FAD-containing aromatic hydroxylase from Pseudomonas azelaica HBP1.
Journal of Biological Chemistry, 272(39):24257-24265

1996

Suske W. A., Kohler H.-P. E., Held M., Wubbolts M. G. and Schmid A. (1996)
2-Hydroxybiphenyl 3-monooxygenase, a novel member of the group of FAD-containing aromatic hydroxylases.
Flavins and Flavoproteins 1996, (Proceedings):383-386

1994

Engesser K.-H., Dohms C. and Schmid A. (1994)
Microbial degradation of model compounds of coal and production of metabolites with potential commercial value.
Fuel Processing Technology, 40(2-3):217-226, DOI-Link

1993

Kohler H.-P. E., Schmid A. and van der Maarel M. (1993)
Metabolism of 2,2’-dihydroxybiphenyl by Pseudomonas sp. strain HBP1: Production and consumption of 2,2’,3-trihydroxybiphenyl.
Journal of Bacteriology, 175(6):1621-1628

Patent Applications

2018

Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A.
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
WO/2018/046104

Karande R., Hoschek A., Heuschkel I., Bühler K., Schmid A.
"A composition of photoautotrophic microorganisms and chemoheterotrophic microorganisms in a biofilm"
PCT/EP2018/074746, filed

2017

Schmid A., Hoschek A., and Bühler B. (2017)
Verfahren zur bioreaktiven Extraktion erzeugten Sauerstoffs aus einem Reaktionsraum, sowie Verwendung von phototrophen Mikroorganismen bei der Gewinnung von Wasserstoff
Germany, DE 10 2017 104 648 A1, filed
International, PCT/EP2018/055449, filed

2016

Karande R., Salamanca D., Engesser K.-H., Buehler K. and Schmid A. (2016)
Microorganisms and a method for the production of lactones and their secondary products by converting cycloalkanes
WO/2018/046104

2012

Fritzsch F. S. O., Howitz S., Blank L. M. and Schmid A. (2012)
Method for the separation of polarisable bioparticles.
PCT/EP2011/057724, filed

2011

Schmid A., Karande R. and Bühler K. (2011)
Segmented Flow Biofilm Reactor.
WO/2012/152337

Schmid A., Bühler B., Ladkau N. and Hermann I. (2011)
Method for omega-aminocarboxylic acid condensation.
Europe, WO/2012/156151 / PCT/EP2012/056251 /priority: 11004029.2 16.05.2011 EP

2010

Pötter M., Schmid A., Bühler B., Hennemann H.-G., Julsing M. K., Schaffer S., Haas T., Schrewe M., Cornelissen S., Roos M. and Häger H. (2010)
Biocatalytic oxidation process with alkL-gene product.
WO 2011/131420 / PCT/EP2011/053834 /priority: 10 2010 015 807.0 20.04.2010 DE

2007

Sadowski G., Schmid A., Brandenbusch C., Bühler B. and Görnert M. (2007)
Method for processing coalescence-inhibited emulsions from whole-cell biotransformations with compressed or supercritical gases.
WO/2009/012754 / EP2170481 / DE 10 2007 034 258.8 / US 8,431,358 B2

Karau A., Sieber V., Haas T., Häger H., Grammann K., Bühler B., Blank L. M. and Schmid A. (2007)
Omega-amino carboxylic acids, omega-amino carboxylic acid esters, or recombinant cells which produce lactams thereof.
WO/2009/077461 / PCT/EP2008/067447 / priority: 102007060705.0 17.12.2007 DE

2002

Schmid A., Hollmann F. and Witholt B. (2002)
Selective functionalization of hydrocarbons with isolated oxygenases and mediator based regeneration.
WO/2004/003210

2001

Schmid A., Hauer B., Bühler B. and Witholt B. (2001)
Method for the oxidation of aromatic compunds.
WO/2002/081718 / PCT/EP2002/003803 / priority: 101 17 359.8 06.04.2001 DE

2000

Schmid A., Hollmann F. and Steckhan E. (2000)
Method comprising the indirect electrochemical regeneration of NAD(P)H.
WO 01/088172

1999

Schmid A., Bühler B., Hauer B. and Witholt B. (1999)
Microbiological method for producing aromatic aldehydes and/or carboxylic acids.
WO/2001/031047 / PCT/EP2000/010552 / priority: 199 51 768.1 27.10.1999 DE

Schmid A., Panke S., Feiten H.-J. and Witholt B. (1999)
Biocatalytic epoxidation of vinylaromatic compounds.
EP 99 203 857.0

Books and Bookchapter

2017

Karande R., Schmid A. and Bühler K. (2017)
Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
Springer, Berlin, Germany (doi:10.1007/978-3-319-31421-1_213-1)

2012

Bühler K. and Schmid A. (2012)
Catalytic biofilms: a powerful concept for future bioprocesses.
in Lear, G. and Lewis, G. Microbial biofilms: Current research and applications.
Caister Academic Press, Norfolk, UK:193 - 222, ISBN: 978-1-904455-96-7

Ütkür F.Ö., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
Regioselective Aromatic Hydroxylation of Quinaldine Using Living Pseudomonas putida Cells Containing Quinaldine 4-oxidase.
in John Whitthal and Peter Sutton Practical Methods in Biocatalysis and Biotransformations 2.
John Wiley & Sons Ltd, Chichester, UK:153-157, ISBN: 978-1-119-99139-7

2011

Brandenbusch C., Bühler B., Sadowski G. and Schmid A. (2011)
Biokatalytische Epoxidierung von Styrol zu enantiomerenreinem (S)-Styroloxid.
in Chmiel H. Bioprozesstechnik.
Spektrum Akademischer Verlag, Heidelberg, Germany:486-488, ISBN: 978-3-8274-2476-1

2009

Bühler K. and Schmid A. (2009)
Biopetrochemicals via biocatalysis by hydrocarbon microbes and their enzymes.
in Kenneth Timmis Handbook of Hydrocarbon and Lipid Microbiology.
Springer, Berlin, Germany, Vol. 1, ISBN: 3540775889

Bühler B., Blank L. M., Ebert B. E., Bühler K. and Schmid A. (2009)
Energy and cofactor issues in fermentation and oxyfunctionalisation processes.
in Smolke C. D. The metabolic pathway engineering handbook.
CRC Press, Boca Raton, Florida, USA, Vol. 2:21 1-32, ISBN: 978-1-4200-7765-0

Bühler K. and Schmid A. (2009)
Synthesis of enantiopure (S)-styrene oxide by selective oxidation of styrene by recombinant E. coli.
in John Witthall and Peter Sutton Practical Methods in Biocatalysis and Biotransformations.
John Wiley & Sons Ltd, Chichester, UK:385-390, ISBN: 978-0-470-51927-1

2008

Eissen M., Geisler G., Bühler B., Fischer C., Hungerbuehler K., Schmid A. and Carreira E. M. (2008)
Mass Balances and Life Cycle Assessment.
in Lapkin A. and Constable D. Green Chemistry Metrics: Measuring and Monitoring Sustainable Processes.
John Wiley & Sons Ltd, Chichester, West Sussex, UK:200-227

2005

Bühler B. and Schmid A. (2005)
Selective enzymatic hydroxylations.
in Dyker G. Handbook of C-H transformations.
Wiley-VCH, Weinheim, Germany, Vol. 2:516-529

2003

Lenke H. and Schmid A. (2003)
Biotransformations.
in Doelle H. Encyclopedia of life support systems.EOLSS Publishers Co. Ltd., Oxford, UK

2002

Hollmann F., Bühler B. and Schmid A. (2002)
Oxidation of alcohols, phenols, aldehydes, acids and C-N bonds.
in Drauz K. and Waldmann H. Enzyme catalysis in organic synthesis.
Wiley-VCH, Weinheim, Germany, Vol. III:1108-1202, 1245-1261,ISBN: 3-527-29949-1

1997

Schmid A. (1997)
PhD Thesis: Der Metabolismus von 2-Hydroxybiphenyl-Verbindungen in Pseudomonas azeleica HBP1: Grundlegende Untersuchungen und ihre biotechnologische Anwendung.
Universitiy of Stuttgart

Teaching

Modul: Molekulare Biotechnologie

Dozent: Prof. Andreas Schmid

WS 2019/2020 (11-BCH-0721)

Beginn: 17.10.2019

Wo: UFZ, Leipzig, Gebäude Kubus, Raum 112

Vorlesung (2 SWS):
17.10., 18.10, 24.10., 25.10., 7.11., 5.12., 12.12., 19.12., 9.1., 16.1., 23.1., 30.1., 6.2., 7.2. (Fallbeispiele von Dr. Willrodt/ BASF AG)

Seminar (1 SWS):
10.1., 17.1., 24.1., 31.1.

Übung:
1.11., 8.11., 6.12.,13.12., 20.12. (und nach Vereinbarung, wenn gewünscht zur Klausurvorbereitung)

Praktikum (5 SWS):
15. Oktober bis 8. November 2019
START: Dienstag, 15. Oktober 2019 im Seminarraum Geb 9.1 (SIAB), 1. OG am UFZ, 10:00 Uhr.
Skript wird nach Möglichkeit vorab zur Vorbereitung verteilt!

Klausur:

Mitte März 2020, nach Absprache

Vorlesungsunterlagen

Head of Department and Group Leader Photobiotechnolgy

Scientific Career

Education

Positions

Publications

Index:

2024 (2)

2023 (3)

2022 (6)

2021 (9)

2020 (3)

2019 (12)

2018 (5)

2017 (11)

2016 (10)

2015 (17)

Publications without UFZ-affiliation

2015

2014

2013

2012

2011

2010

2009

2008

2007

2006

2005

2004

2003

2002

2001

2000

1999

1998

1997

1996

1994

1993

Patent Applications

2018

2017

2016

2012

2011

2010

2007

2002

2001

2000

1999

Books and Bookchapter

2017

2012

2011

2009

2008

2005

2003

2002

1997

Teaching

Head of Department and
Group Leader Photobiotechnolgy